DIRC1 (NM_052952) Human Tagged ORF Clone

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SKU
RC215765
DIRC1 (Myc-DDK-tagged)-Human disrupted in renal carcinoma 1 (DIRC1)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DIRC1
Synonyms disrupted in renal carcinoma 1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215765 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGAGGCCCACATGCAGCCTGCAAAACTGCAAACATCACTGCCCACCACAGACCACGGAAGCAAGA
AGCCTGTTTCCTGTTACTTGCCACCACTCAGCAATGCCCACCCCATGTGTATAGAGGTCCAGAATGCTCA
AAATTGTTCTTCTGCTGCTGCAACATTGGAGCCTTCCATCATCTCAGACACATGCTTTTATAAACCAATA
ACAAAGGATCAACTTTCTTCTAGGTCTGAATTGAATACTGTGAGGCTAAAGTGTCTTAACTCATTGAGGG
GGTGGAAAATATTAAACCAACTAAGCCTTACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215765 protein sequence
Red=Cloning site Green=Tags(s)

MPEAHMQPAKLQTSLPTTDHGSKKPVSCYLPPLSNAHPMCIEVQNAQNCSSAAATLEPSIISDTCFYKPI
TKDQLSSRSELNTVRLKCLNSLRGWKILNQLSLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_052952
ORF Size 312 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_052952.3
RefSeq Size 1454 bp
RefSeq ORF 315 bp
Locus ID 116093
Cytogenetics 2q32.2
MW 11.4 kDa
Summary Disruption of DIRC1 by translocation is associated with familial clear cell renal cancer. Overexpression of DIRC1 is associated with tumor progression and poor prognosis in gastric cancer. provided by RefSeq, Mar 2019
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Documents
Resources
"NM_052952" in other vectors (4)
SKU Description Size Price
RC215765L3 Lenti ORF clone of Human disrupted in renal carcinoma 1 (DIRC1), Myc-DDK-tagged 10 ug
$450.00
RC215765L4 Lenti ORF clone of Human disrupted in renal carcinoma 1 (DIRC1), mGFP tagged 10 ug
$450.00
RG215765 DIRC1 (tGFP-tagged) - Human disrupted in renal carcinoma 1 (DIRC1) 10 ug
$489.00
SC305703 DIRC1 (untagged)-Human disrupted in renal carcinoma 1 (DIRC1) 10 ug
$165.00

Plasmid Map for RC215765

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Cloning scheme for RC215765

Circular map for RC215765

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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