CENPJ (NM_018451) Human Tagged ORF Clone
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CENPJ |
| Synonyms | BM032; CENP-J; CPAP; LAP; LIP1; MCPH6; Sas-4; SASS4; SCKL4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_018451 |
| ORF Size | 4014 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_018451.5 |
| RefSeq Size | 4387 bp |
| RefSeq ORF | 4017 bp |
| Locus ID | 55835 |
| UniProt ID | Q9HC77, A8K8P1 |
| Cytogenetics | 13q12.12-q12.13 |
| MW | 152.8 kDa |
| Summary | This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and cognitive disability. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Apr 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_018451" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215682L1 | Lenti ORF clone of Human centromere protein J (CENPJ), Myc-DDK-tagged | 10 ug |
$1,444.00
|
|
| RC215682L2 | Lenti ORF clone of Human centromere protein J (CENPJ), mGFP tagged | 10 ug |
$1,444.00
|
|
| RC215682L3 | Lenti ORF clone of Human centromere protein J (CENPJ), Myc-DDK-tagged | 10 ug |
$1,444.00
|
|
| RC215682L4 | Lenti ORF clone of Human centromere protein J (CENPJ), mGFP tagged | 10 ug |
$1,444.00
|
|
| RG215682 | CENPJ (tGFP-tagged) - Human centromere protein J (CENPJ) | 10 ug |
$789.00
$1,344.00
|
|
| SC308859 | CENPJ (untagged)-Human centromere protein J (CENPJ) | 10 ug |
$1,145.00
|
|
Citations
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