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CENPJ (NM_018451) Human Untagged Clone

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SKU
SC308859
CENPJ (untagged)-Human centromere protein J (CENPJ)
  $1,145.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CENPJ
Synonyms BM032; CENP-J; CPAP; LAP; LIP1; MCPH6; Sas-4; SASS4; SCKL4
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_018451 edited
ATTGTGGCGGCCCATTTGTAAATGCTGCGGAGATTGAGGTGTCGGTTCGTGCTGCTGAGC
TGCCCAGGCTTCACGGAGCGGTGTTGGAAATCAATAGCTCTTCTAGCCTTTGCATTGTTT
AAATATAATAGTGTCATTGGACTAAGATGTTCCTGATGCCAACCTCTTCAGAGTTAAACA
GTGGGCAGAACTTCCTAACCCAGTGGGTGACCAATCCTTCTCGGGCTGGGGTCATATTAA
ATCGTGGATTTCCTATTTTGGAAGCAGACAAAGAGAAGCGAGCAGCTGTGGACATTTCTA
CCAGCTTTCCTATTAAAGGCACACATTTTTCTGATAGCTTCAGCTTTATAAATGAAGAAG
ATTCACTTCTTGAAGAACAGAAGTTGGAGTCAAACAACCCTTACAAACCACAGTCAGATA
AATCTGAAACCCATACAGCCTTTCCTTGCATTAAAAAGGGACCACAGGTAGCGGCATGTC
ACAGTGCTCCTGGACACCAGGAAGAAAACAAAAATGACTTCATCCCAGATCTTGCGAGTG
AATTCAAAGAAGGGGCTTATAAAGACCCACTTTTTAAAAAACTTGAACAGCTGAAAGAAG
TACAACAGAAGAAGCAGGAACAATTGAAGAGGCAACAGTTGGAGCAACTACAGAGACTCA
TGGAAGAACAAGAGAAGCTGCTCACCATGGTGTCTGGGCAGTGCACACTTCCAGGTTTGA
GTTTACTGCCTGATGATCAGAGCCAGAAGCACAGGTCTCCAGGAAATACCACCACTGGAG
AGAGAGCCACATGCTGCTTCCCATCATATGTCTACCCGGACCCAACCCAGGAAGAAACAT
ACCCGTCCAACATTTTATCCCATGAGCAAAGCAACTTCTGTAGAACTGCTCATGGAGATT
TTGTCTTAACTTCAAAACGTGCGTCTCCTAATTTATTTTCTGAGGCACAGTATCAAGAAG
CACCTGTGGAAAAAAATAATTTAAAAGAAGAAAACCGTAACCATCCTACAGGAGAAAGTA
TCTTATGTTGGGAGAAAGTGACAGAACAGATTCAGGAAGCAAATGATAAGAACTTACAAA
AACATGATGATTCCTCAGAAGTGGCTAATATTGAAGAAAGGCCCATTAAAGCTGCTATTG
GAGAAAGGAAACAGACCTTTGAAGATTACTTAGAAGAACAAATTCAGTTGGAAGAGCAAG
AACTGAAGCAAAAACAGCTGAAGGAAGCAGAAGGACCATTGCCAATCAAAGCAAAACCAA
AACAACCATTTTTAAAACGAGGAGAAGGTTTAGCTAGATTTACTAATGCCAAATCTAAGT
TTCAAAAAGGCAAAGAAAGTAAACTAGTGACTAACCAGAGCACTTCCGAGGACCAGCCGC
TGTTTAAAATGGATAGACAGCAACTCCAGCGGAAAACCGCTCTTAAAAATAAAGAGCTGT
GTGCAGACAACCCTATCCTTAAAAAGGACAGTAAAGCTAGAACCAAGAGTGGTTCTGTCA
CCCTCAGTCAGAAGCCGAAAATGCTGAAGTGTAGTAACAGGAAAAGTCTTTCTCCGTCAG
GATTGAAAATACAGACGGGGAAGAAATGTGATGGGCAGTTTAGAGACCAGATCAAATTTG
AAAACAAAGTCACATCTAATAATAAAGAAAATGTAACTGAGTGTCCAAAACCTTGCGATA
CTGGCTGCACAGGGTGGAATAAGACACAAGGTAAAGACAGACTTCCTCTTTCAACAGGGC
CGGCCAGCCGGCTGGCTGCTAAGAGCCCCATAAGGGAGACCATGAAAGAGTCTGAATCTT
CTCTTGACGTTTCTCTTCAGAAAAAGTTAGAGACTTGGGAACGAGAAAAGGAAAAGGAAA
ATTTGGAATTAGATGAATTTTTGTTTTTAGAACAAGCTGCTGATGAAATATCATTTTCTA
GTAATTCCTCATTTGTACTGAAAATCTTAGAAAGGGATCAACAGATCTGCAAAGGTCACC
GGATGTCTTCCACCCCTGTCAAAGCTGTGCCACAGAAGACAAATCCGGCAGATCCCATTA
GTCATTGTAACCGCAGTGAGGATTTGGACCACACTGCACGTGAGAAGGAGAGTGAGTGTG
AAGTCGCACCCAAACAACTTCATTCATTGTCCTCAGCTGATGAATTGAGGGAACAGCCTT
GTAAAATCAGGAAAGCCGTCCAAAAGAGCACTTCTGAAAATCAGACTGAATGGAATGCAC
GTGACGATGAAGGTGTTCCAAATAGTGACAGTAGCACTGACTCTGAGGAACAGCTTGATG
TTACCATAAAACCATCGACTGAGGATAGAGAGAGGGGCATCAGCAGCAGAGAGGATAGCC
CACAAGTCTGTGATGATAAGGGGCCTTTTAAGGACACCAGGACCCAAGAAGATAAAAGGA
GAGATGTTGATCTGGATTTGTCTGATAAAGATTACAGTAGCGATGAGTCTATCATGGAAA
GCATAAAACATAAAGTGTCTGAGCCCTCGAGATCCTCATCCCTAAGTCTGAGTAAAATGG
ACTTTGATGATGAAAGAACTTGGACTGACCTTGAAGAGAATTTGTGTAACCATGATGTTG
TTCTTGGGAATGAATCCACTTATGGGACGCCGCAGACATGCTACCCTAATAATGAAATAG
GTATCCTGGACAAAACAATAAAAAGGAAGATTGCACCAGTCAAGAGGGGAGAAGACTTGA
GCAAGTCCAGGAGGAGCAGAAGTCCTCCTACATCGGAGCTGATGATGAAATTCTTTCCTT
CTTTGAAACCAAAACCAAAGTCAGATTCACACTTGGGAAATGAACTCAAGTTAAACATAA
GTCAAGACCAACCACCTGGTGACAATGCTCGATCCCAGGTTTTGAGAGAGAAAATTATTG
AATTGGAAACAGAAATAGAAAAGTTTAAAGCTGAGAACGCATCTTTAGCTAAACTTCGCA
TTGAACGAGAAAGTGCCTTGGAAAAACTCAGGAAAGAAATTGCAGACTTCGAACAACAGA
AAGCAAAAGAATTAGCTCGAATAGAAGAGTTTAAAAAGGAGGAGATGAGGAAGCTACAAA
AGGAACGTAAAGTTTTTGAAAAGTATACTACAGCTGCAAGAACTTTTCCAGATAAAAAGG
AACGTGAAGAAATACAGACTTTAAAACAGCAAATAGCAGATTTACGGGAAGATTTGAAAA
GAAAGGAGACCAAATGGTCAAGTACACACAGCCGTCTCAGAAGCCAGATACAAATGTTAG
TCAGAGAGAACACAGACCTCCGGGAAGAAATAAAAGTGATGGAAAGATTCCGACTGGATG
CCTGGAAGAGAGCAGAAGCCATAGAGAGCAGCCTCGAGGTGGAGAAGAAGGACAAGCTTG
CGAACACATCTGTTCGATTTCAAAACAGTCAGATTTCTTCAGGAACCCAGGTAGAAAAAT
ACAAGAAAAATTATCTTCCAATGCAAGGCAATCCACCTCGAAGATCCAAGTCTGCACCTC
CTCGTGATTTAGGCAATTTGGATAAGGGACAAGCTGCCTCTCCCAGGGAGCCACTTGAAC
CACTGAACTTCCCAGATCCTGAATATAAAGAGGAGGAGGAAGACCAAGACATACAGGGAG
AAATCAGTCATCCTGATGGAAAGGTGGAAAAGGTTTATAAGAATGGGTGCCGTGTTATAC
TGTTTCCCAATGGAACTCGAAAGGAAGTGAGTGCAGATGGGAAGACCATCACTGTCACTT
TCTTTAATGGTGACGTGAAGCAGGTCATGCCAGACCAAAGAGTGATCTACTACTATGCAG
CTGCCCAGACCACTCACACGACATACCCGGAGGGACTGGAAGTCTTACATTTCTCAAGTG
GACAAATAGAAAAACATTACCCAGATGGAAGAAAAGAAATCACGTTTCCTGACCAGACTG
TTAAAAACTTATTTCCTGATGGACAAGAAGAAAGCATTTTCCCAGATGGTACAATTGTCA
GAGTACAACGTGATGGCAACAAACTCATAGAGTTTAATAATGGCCAAAGAGAACTACATA
CTGCCCAGTTCAAGAGACGGGAATACCCAGATGGCACTGTTAAAACCGTATATGCAAACG
GTCATCAAGAAACGAAGTACAGATCCGGTCGGATAAGAGTTAAGGACAAGGAGGGTAATG
TGCTAATGGACACGGAGCTGTGACGATCCTCATGTGATCATGAAGTAACAGTAACTGACT
TTTTATGTTAAAAAATGTACATTTACTGTGGATTCTGTTTAATTTATTGTGTATGTGTGG
GGAAAAGATTGGATTCTAAAATAAAAGTTTACCCTGTGGCAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAA
Restriction Sites NotI-NotI |
ACCN NM_018451
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018451.2, NP_060921.2
RefSeq Size 4387 bp
RefSeq ORF 4017 bp
Locus ID 55835
UniProt ID Q9HC77
Cytogenetics 13q12.12-q12.13
Summary This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and cognitive disability. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Apr 2012
Transcript Variant: This variant (1) represents the protein coding transcript.
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Documents
Resources
"NM_018451" in other vectors (6)
SKU Description Size Price
RC215682 CENPJ (Myc-DDK-tagged)-Human centromere protein J (CENPJ) 10 ug
$1,144.00
RC215682L1 Lenti ORF clone of Human centromere protein J (CENPJ), Myc-DDK-tagged 10 ug
$1,444.00
RC215682L2 Lenti ORF clone of Human centromere protein J (CENPJ), mGFP tagged 10 ug
$1,444.00
RC215682L3 Lenti ORF clone of Human centromere protein J (CENPJ), Myc-DDK-tagged 10 ug
$1,444.00
RC215682L4 Lenti ORF clone of Human centromere protein J (CENPJ), mGFP tagged 10 ug
$1,444.00
RG215682 CENPJ (tGFP-tagged) - Human centromere protein J (CENPJ) 10 ug
$789.00 $1,344.00

file-alt Citations

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