NAT8B (NM_016347) Human Tagged ORF Clone
SKU
RC215647
NAT8B (Myc-DDK-tagged)-Human N-acetyltransferase 8B (GCN5-related, putative, gene/pseudogene) (NAT8B)
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NAT8B |
| Synonyms | CML2; Hcml2; NAT8BP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016347 |
| ORF Size | 681 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016347.1, NP_057431.1 |
| RefSeq Size | 845 bp |
| RefSeq ORF | 684 bp |
| Locus ID | 51471 |
| UniProt ID | Q9UHF3 |
| Cytogenetics | 2p13.1 |
| MW | 25.4 kDa |
| Summary | The protein encoded by this gene is highly similar to the N-acetyltransferase 8 (NAT8) gene product, which is a kidney and liver protein with homology to bacterial acetyltransferases involved in drug resistance. This gene is localized on chromosome 2 in the vicinity of the NAT8 gene and may represent a pseudogene of NAT8. This gene contains two polymorphic nonsense mutations that disrupt the active site of the protein. The full-length product of this gene contains a complete acetyltransferase domain and is identical in length to NAT8. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016347" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215647L1 | Lenti ORF clone of Human N-acetyltransferase 8B (GCN5-related, putative, gene/pseudogene) (NAT8B), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC215647L2 | Lenti ORF clone of Human N-acetyltransferase 8B (GCN5-related, putative, gene/pseudogene) (NAT8B), mGFP tagged | 10 ug |
$600.00
|
|
| RC215647L3 | Lenti ORF clone of Human N-acetyltransferase 8B (GCN5-related, putative, gene/pseudogene) (NAT8B), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC215647L4 | Lenti ORF clone of Human N-acetyltransferase 8B (GCN5-related, putative, gene/pseudogene) (NAT8B), mGFP tagged | 10 ug |
$600.00
|
|
| RG215647 | NAT8B (tGFP-tagged) - Human N-acetyltransferase 8B (GCN5-related, putative, gene/pseudogene) (NAT8B) | 10 ug |
$489.00
$500.00
|
|
| SC311351 | NAT8B (untagged)-Human N-acetyltransferase 8B (GCN5-related, putative, gene/pseudogene) (NAT8B) | 10 ug |
$300.00
|
|
Citations
Overexpression of ATase1 and ATase2 disrupts the secretome and causes a progeria phenotype
Cheng, Wu, Haque et al
Life Sci Alliance (2025) 8 (12) DOI: 10.26508/lsa.202503378
Cheng, Wu, Haque et al
Life Sci Alliance (2025) 8 (12) DOI: 10.26508/lsa.202503378
Cited by 4
AI Summary: OriGene’s ATase1/NAT8B (RC215647) and ATase2/NAT8 (RC202157) pCMV6 plasmids were used as sources of human cDNA to generate constructs for the overexpression studies in this paper.
Snippet: ated as described (Hullinger et al, 2016;Rigby et al, 2022a, 2022b). In brief, human cDNA was isolated from ATase1- and ATase2-pCMV6 plasmids (RC215647 for ATase1/NAT8B and RC202157 for ATase2/NAT8;Origene) using PCR and subsequently subcloned into pTRE-Tight plasmid (Takara Bio, Inc.). The resulting pTRE-Tight-ATase1 and pTRE-Tight-ATase2 plasmids were linearized with XhoI and then injected (3
Increased expression of ATase1/NAT8B or ATase2/NAT8 in the mouse results in an autistic-like phenotype with altered dendritic branching and spine formation
Kalimuthu, Lu, Steenhagen et al
Mol Psychiatry (2025)
Kalimuthu, Lu, Steenhagen et al
Mol Psychiatry (2025)
Cited by 1
AI Summary: OriGene products ATase1-pCMV6 (RC215647) and ATase2-pCMV6 plasmids provided the human cDNAs used to generate neuron-specific overexpression mouse models in this study.
Snippet: Camk2a-tTA;TRE-ATase1 (referred to as ATase1 nTg thereafter) and Camk2a-tTA;TRE-ATase2 (referred to as ATase2 nTg thereafter) mice were generated as previously described [12, 20, 21]. Human cDNA was isolated from ATase1- and ATase2-pCMV6 plasmids (Origene;RC215647 for
The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathway
Rigby, Ding, Farrugia et al
J. Neurochem. (2020)
Rigby, Ding, Farrugia et al
J. Neurochem. (2020)
Cited by 16
AI Summary: OriGene products—including the anti-DDK antibody (TA50011), pCMV6-entry plasmid backbone (PS100001), and expression plasmids for human NAT8B (RC215647), NAT8 (RC203157), CREB1 (RC210577), c-FOS (RC202597), c-JUN (RC209804), and p53 (RC200003)—were used for protein detection and gene overexpression experiments that enabled the study of regulatory mechanisms controlling ATase1/NAT8B and ATase2/NAT8 in the secretory pathway.
Snippet: lysine (Abcam #ab409;RRID:AB_308707), anti-DDK (Origene TA50011;RRID:AB_2622345), and anti-Myc (Santa Cruz Biotechnology sc-40;RRID:AB_627268) (pCMV6-entry backbone;OriGene #PS100001;2017) or empty plasmid (serving as a control). … pCMV6-entry empty plasmid (OriGene, #PS100001;2017);cells were harvested 48 hr after transfection. PC12 stable lines were generated by transfecting with Lipofectamine 2000 using the following plasmids: pCMV6-entry empty plasmid (described above), human NAT8B-pCMV6 (OriGene, #RC215647;2018), or human NAT8-pCMV6 (OriGene, #RC203157;2018)...DDK (Origene, TA50011;RRID:AB_2622345)...transcription factor expression plasmid (pCMV6-entry backbone;OriGene, #PS100001;2017)...human CREB1-pCMV6 (Origene, #RC210577;2017), human c-FOS-pCMV6 (Origene, #RC202597;2017), human c-JUN-pCMV6 (Origene, #RC209804;2017), p53-pCMV6 (OriGene, #RC200003;2019)
Showing 1-3 of 3 papers.
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