CDCP1 (NM_178181) Human Tagged ORF Clone

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SKU
RC215553
CDCP1 (Myc-DDK-tagged)-Human CUB domain containing protein 1 (CDCP1), transcript variant 2
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDCP1
Synonyms CD318; SIMA135; TRASK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215553 representing NM_178181
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCCTGAACTGCGGGGTCTCTATCGCACTGCTAGGGGTTCTGCTGCTGGGTGCGGCGCGCCTGC
CGCGCGGGGCAGAAGCTTTTGAGATTGCTCTGCCACGAGAAAGCAACATTACAGTTCTCATAAAGCTGGG
GACCCCGACTCTGCTGGCAAAACCCTGTTACATCGTCATTTCTAAAAGACATATAACCATGTTGTCCATC
AAGTCTGGAGAAAGAATAGTCTTTACCTTTAGCTGCCAGAGTCCTGAGAATCACTTTGTCATAGAGATCC
AGAAAAATATTGACTGTATGTCAGGCCCATGTCCTTTTGGGGAGGTTCAGCTTCAGCCCTCGACATCGTT
GTTGCCTACCCTCAACAGAACTTTCATCTGGGATGTCAAAGCTCATAAGAGCATCGGTTTAGAGCTGCAG
TTTTCCATCCCTCGCCTGAGGCAGATCGGTCCGGGTGAGAGCTGCCCAGACGGAGTCACTCACTCCATCA
GCGGCCGAATCGATGCCACCGTGGTCAGGATCGGAACCTTCTGCAGCAATGGCACTGTGTCCCGGATCAA
GATGCAAGAAGGAGTGAAAATGGCCTTACACCTCCCATGGTTCCACCCCAGAAATGTCTCCGGCTTCAGC
ATTGCAAACCGCTCATCTATAAAACGTCTGTGCATCATCGAGTCTGTGTTTGAGGGTGAAGGCTCAGCAA
CCCTGATGTCTGCCAACTACCCAGAAGGCTTCCCTGAGGATGAGCTCATGACGTGGCAGTTTGTCGTTCC
TGCACACCTGCGGGCCAGCGTCTCCTTCCTCAACTTCAACCTCTCCAACTGTGAGAGGAAGGAGGAGCGG
GTTGAATACTACATCCCGGGCTCCACCACCAACCCCGAGGTGTTCAAGCTGGAGGACAAGCAGCCTGGGA
ACATGGCGGGGAACTTCAACCTCTCTCTGCAAGGCTGTGACCAAGATGCCCAAAGTCCAGGGATCCTCCG
GCTGCAGTTCCAAGTTTTGGTCCAACATCCACAAAATGAAAGCAGTGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215553 representing NM_178181
Red=Cloning site Green=Tags(s)

MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVISKRHITMLSI
KSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWDVKAHKSIGLELQ
FSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHPRNVSGFS
IANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVVPAHLRASVSFLNFNLSNCERKEER
VEYYIPGSTTNPEVFKLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESSE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178181
ORF Size 1029 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178181.2
RefSeq Size 1410 bp
RefSeq ORF 1032 bp
Locus ID 64866
UniProt ID Q9H5V8
Cytogenetics 3p21.31
Protein Families ES Cell Differentiation/IPS, Transmembrane
MW 37.6 kDa
Summary This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. provided by RefSeq, May 2013
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Documents
Resources
"NM_178181" in other vectors (4)
SKU Description Size Price
RC215553L3 Lenti ORF clone of Human CUB domain containing protein 1 (CDCP1), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC215553L4 Lenti ORF clone of Human CUB domain containing protein 1 (CDCP1), transcript variant 2, mGFP tagged 10 ug
$757.00
RG215553 CDCP1 (tGFP-tagged) - Human CUB domain containing protein 1 (CDCP1), transcript variant 2 10 ug
$489.00 $657.00
SC123355 CDCP1 (untagged)-Human CUB domain containing protein 1 (CDCP1), transcript variant 2 10 ug
$457.00

Plasmid Map for RC215553

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Cloning scheme for RC215553

Circular map for RC215553

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