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ELMO1 (NM_014800) Human Tagged ORF Clone

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SKU
RC215541
ELMO1 (Myc-DDK-tagged)-Human engulfment and cell motility 1 (ELMO1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $665.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ELMO1
Synonyms CED-12; CED12; ELMO-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215541 representing NM_014800
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCCACCCGCGGACATCGTCAAGGTGGCCATAGAATGGCCGGGCGCCTACCCCAAACTCATGGAAA
TTGATCAGAAAAAACCACTGTCTGCAATAATAAAGGAAGTCTGTGATGGGTGGTCTCTTGCCAACCATGA
ATATTTTGCACTCCAGCATGCCGATAGTTCAAACTTCTATATCACAGAAAAGAACCGCAATGAGATAAAA
AATGGCACTATCCTTCGATTAACCACATCTCCAGCTCAGAACGCCCAGCAGCTCCATGAACGAATCCAGT
CCTCGAGTATGGATGCCAAGCTGGAAGCCCTGAAGGACTTGGCCAGCCTCTCCCGGGATGTCACGTTTGC
CCAGGAGTTTATAAACCTGGACGGTATCTCTCTCCTCACGCAGATGGTAGAGAGCGGCACTGAGCGATAC
CAGAAATTGCAGAAGATCATGAAGCCTTGCTTTGGAGACATGCTGTCCTTCACCCTGACGGCCTTCGTTG
AGCTGATGGACCATGGCATAGTGTCCTGGGATACATTTTCGGTGGCGTTCATTAAGAAGATAGCAAGTTT
TGTGAACAAGTCAGCCATAGACATCTCGATCCTGCAGCGGTCCTTGGCCATTTTGGAGTCGATGGTGCTC
AATAGCCATGACCTCTACCAGAAAGTGGCGCAGGAGATCACCATCGGCCAGCTCATTCCACACCTGCAAG
GGTCAGATCAAGAAATCCAAACCTATACTATTGCAGTGATTAATGCGCTTTTCCTGAAGGCTCCTGATGA
GAGGAGGCAGGAGATGGCGAATATTTTGGCTCAGAAGCAACTGCGTTCCATCATTTTAACACATGTCATC
CGAGCCCAGCGGGCCATCAACAATGAGATGGCGCACCAGCTGTATGTTCTACAAGTGCTCACCTTTAACC
TCCTGGAAGACAGGATGATGACCAAAATGGACCCCCAGGACCAGGCTCAGAGGGACATCATATTTGAACT
TCGAAGAATTGCTTTTGATGCTGAGTCTGAACCTAACAACAGCAGTGGCAGCATGGAGAAACGCAAGTCC
ATGTACACGCGAGATTATAAGAAGCTTGGGTTCATTAATCATGTCAACCCTGCCATGGACTTCACGCAGA
CTCCACCTGGGATGTTGGCTCTGGACAACATGCTGTACTTTGCCAAGCACCACCAAGATGCCTACATCCG
GATTGTGCTTGAGAACAGTAGTCGAGAAGACAAGCATGAATGTCCCTTTGGCCGCAGTAGTATAGAGCTG
ACCAAGATGCTATGTGAGATCTTGAAAGTGGGCGAGTTGCCTAGTGAGACCTGCAACGACTTCCACCCGA
TGTTCTTCACCCACGACAGATCCTTTGAGGAGTTTTTCTGCATCTGTATCCAGCTCCTGAACAAGACATG
GAAGGAAATGAGGGCAACTTCTGAAGACTTCAACAAGGTAATGCAGGTGGTGAAGGAGCAGGTTATGAGA
GCACTTACAACCAAGCCTAGCTCCCTGGACCAGTTCAAGAGCAAACTGCAGAACCTGAGCTACACTGAGA
TCCTGAAAATCCGCCAGTCCGAGAGGATGAACCAGGAAGATTTCCAGTCCCGCCCGATTTTGGAACTAAA
GGAGAAGATTCAGCCAGAAATCTTAGAGCTGATCAAACAGCAACGCCTGAACCGCCTTGTGGAAGGGACC
TGCTTTAGGAAACTCAATGCCCGGCGGAGGCAAGACAAGTTTTGGTATTGTCGGCTTTCGCCAAATCACA
AAGTCCTGCATTACGGAGACTTAGAAGAGAGTCCTCAGGGAGAAGTGCCCCACGATTCCTTGCAGGACAA
ACTGCCGGTGGCAGATATCAAAGCCGTGGTGACGGGAAAGGACTGCCCTCATATGAAAGAGAAAGGTGCC
CTTAAACAAAACAAGGAGGTGCTTGAACTCGCTTTCTCCATCTTGTATGACTCAAACTGCCAACTGAACT
TCATCGCTCCTGACAAGCATGAGTACTGTATCTGGACGGATGGACTGAATGCGCTACTCGGGAAGGACAT
GATGAGCGACCTGACGCGGAATGACCTGGACACCCTGCTCAGCATGGAAATCAAGCTCCGCCTCCTGGAC
CTGGAAAACATCCAGATCCCTGACGCACCTCCGCCGATTCCCAAGGAGCCCAGCAACTATGACTTCGTCT
ATGACTGTAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215541 representing NM_014800
Red=Cloning site Green=Tags(s)

MPPPADIVKVAIEWPGAYPKLMEIDQKKPLSAIIKEVCDGWSLANHEYFALQHADSSNFYITEKNRNEIK
NGTILRLTTSPAQNAQQLHERIQSSSMDAKLEALKDLASLSRDVTFAQEFINLDGISLLTQMVESGTERY
QKLQKIMKPCFGDMLSFTLTAFVELMDHGIVSWDTFSVAFIKKIASFVNKSAIDISILQRSLAILESMVL
NSHDLYQKVAQEITIGQLIPHLQGSDQEIQTYTIAVINALFLKAPDERRQEMANILAQKQLRSIILTHVI
RAQRAINNEMAHQLYVLQVLTFNLLEDRMMTKMDPQDQAQRDIIFELRRIAFDAESEPNNSSGSMEKRKS
MYTRDYKKLGFINHVNPAMDFTQTPPGMLALDNMLYFAKHHQDAYIRIVLENSSREDKHECPFGRSSIEL
TKMLCEILKVGELPSETCNDFHPMFFTHDRSFEEFFCICIQLLNKTWKEMRATSEDFNKVMQVVKEQVMR
ALTTKPSSLDQFKSKLQNLSYTEILKIRQSERMNQEDFQSRPILELKEKIQPEILELIKQQRLNRLVEGT
CFRKLNARRRQDKFWYCRLSPNHKVLHYGDLEESPQGEVPHDSLQDKLPVADIKAVVTGKDCPHMKEKGA
LKQNKEVLELAFSILYDSNCQLNFIAPDKHEYCIWTDGLNALLGKDMMSDLTRNDLDTLLSMEIKLRLLD
LENIQIPDAPPPIPKEPSNYDFVYDCN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014800
ORF Size 2181 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014800.9, NP_055615.8
RefSeq Size 3727 bp
RefSeq ORF 2184 bp
Locus ID 9844
UniProt ID Q92556, A4D1X5
Cytogenetics 7p14.2-p14.1
Domains DUF609
Protein Pathways Chemokine signaling pathway
MW 83.6 kDa
Summary This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2013
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Documents
Resources
"NM_014800" in other vectors (6)
SKU Description Size Price
RC215541L1 Lenti ORF clone of Human engulfment and cell motility 1 (ELMO1), transcript variant 1, Myc-DDK-tagged 10 ug
$966.00
RC215541L2 Lenti ORF clone of Human engulfment and cell motility 1 (ELMO1), transcript variant 1, mGFP tagged 10 ug
$966.00
RC215541L3 Lenti ORF clone of Human engulfment and cell motility 1 (ELMO1), transcript variant 1, Myc-DDK-tagged 10 ug
$966.00
RC215541L4 Lenti ORF clone of Human engulfment and cell motility 1 (ELMO1), transcript variant 1, mGFP tagged 10 ug
$966.00
RG215541 ELMO1 (tGFP-tagged) - Human engulfment and cell motility 1 (ELMO1), transcript variant 1 10 ug
$866.00
SC316256 ELMO1 (untagged)-Human engulfment and cell motility 1 (ELMO1), transcript variant 1 10 ug
$667.00

Plasmid Map for RC215541

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215541
Circular map for RC215541

file-alt Citations

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