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TEF1 (TEAD1) (NM_021961) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC215492L1
Lenti-ORF clone of TEAD1 (Myc-DDK-tagged)-Human TEA domain family member 1 (SV40 transcriptional enhancer factor) (TEAD1)
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $986.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TEF1
Synonyms AA; NTEF-1; REF1; TCF-13; TCF13; TEAD-1; TEF-1
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC215492).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021961
ORF Size 1278 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021961.5
RefSeq Size 9433 bp
RefSeq ORF 1281 bp
Locus ID 7003
UniProt ID P28347
Cytogenetics 11p15.3
Domains TEA
Protein Families Transcription Factors
MW 47.9 kDa
Summary This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. provided by RefSeq, May 2010
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"NM_021961" in other vectors (6)
SKU Description Size Price
RC215492 TEAD1 (Myc-DDK-tagged)-Human TEA domain family member 1 (SV40 transcriptional enhancer factor) (TEAD1) 10 ug
$289.00 $686.00
RC215492L2 Lenti-ORF clone of TEAD1 (mGFP-tagged)-Human TEA domain family member 1 (SV40 transcriptional enhancer factor) (TEAD1) 10 ug
$986.00
RC215492L3 Lenti-ORF clone of TEAD1 (Myc-DDK-tagged)-Human TEA domain family member 1 (SV40 transcriptional enhancer factor) (TEAD1) 10 ug
$986.00
RC215492L4 Lenti-ORF clone of TEAD1 (mGFP-tagged)-Human TEA domain family member 1 (SV40 transcriptional enhancer factor) (TEAD1) 10 ug
$986.00
RG215492 TEAD1 (tGFP-tagged) - Human TEA domain family member 1 (SV40 transcriptional enhancer factor) (TEAD1) 10 ug
$489.00 $886.00
SC310426 TEAD1 (untagged)-Human TEA domain family member 1 (SV40 transcriptional enhancer factor) (TEAD1) 10 ug
$732.00

Plasmid Map for RC215492L1

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Cloning scheme for RC215492L1
Circular map for RC215492L1

file-alt Citations

Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma
Tome-Garcia, Erfani, Nudelman et al
Nat Commun (2018) 9 (1), 4020 DOI: 10.1038/s41467-018-06258-2
AI Summary: OriGene’s Myc-DDK-tagged human ORF lentivirus constructs for TEAD1 (RC215492L1), AQP4 (RC204693L1), and CDH11 (RC203810L1) were used for overexpression studies to investigate the roles of these genes in glioblastoma cell migration.
Snippet: For overexpression studies, the (Myc-DDK-tagged)-TEAD1, -AQP4, and CDH11 human ORF lentivirus constructs were transfected and purified from Lenti-X 293T cells (Origene RC215492L1, RC204693L1, and RC203810L1, respectively). GBM cells were infected for 12 h and allowed to recover for at least 48 h, prior to analysis
FigureFigureFigure
Showing 1-1 of 1 papers.
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