LAMB4 (NM_007356) Human Tagged ORF Clone
$1,480.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | LAMB4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007356 |
| ORF Size | 5283 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007356.1, NP_031382.1 |
| RefSeq Size | 5874 bp |
| RefSeq ORF | 5286 bp |
| Locus ID | 22798 |
| UniProt ID | A4D0S4 |
| Cytogenetics | 7q31.1 |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer |
| MW | 193.4 kDa |
| Summary | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
Laminin β4 is required for the development of human peripheral sensory neurons
Saito-Diaz, Saini, Patel et al
Development (2026) 153 (16) DOI: 10.1242/dev.205371
Saito-Diaz, Saini, Patel et al
Development (2026) 153 (16) DOI: 10.1242/dev.205371
Cited by 3
AI Summary: OriGene’s tagged ORF clone for LAMB4 (RC215486) was used as the template to PCR-amplify LAMB4 for functional studies, enabling the authors to investigate its essential role in human peripheral sensory neuron development.
Snippet: ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="pmc:entrez-nucleotide" xlink:href="NM_007356">NM_007356) was PCR-amplified from a commercially obtained tagged ORF clone (OriGene, RC215486) using the following primers: forward, 5′-GGCACCAAAATCAACGGGAC-3′;reverse, 5′-TGACTCTCGAGTCAGCTATAGCACCTAGCATATTTTTTTTCTTGT-3′. The amplified product was cloned into the pEN
Showing 1-1 of 1 papers.
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