TCF3 / E2A (TCF3) (NM_003200) Human Tagged ORF Clone
SKU
RC215432
TCF3 (Myc-DDK-tagged)-Human transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 1
$609.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TCF3 / E2A |
| Synonyms | AGM8; bHLHb21; E2A; E47; ITF1; p75; TCF-3; VDIR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003200 |
| ORF Size | 1962 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003200.5 |
| RefSeq Size | 4396 bp |
| RefSeq ORF | 1965 bp |
| Locus ID | 6929 |
| UniProt ID | P15923 |
| Cytogenetics | 19p13.3 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors |
| MW | 67.4 kDa |
| Summary | This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. provided by RefSeq, Sep 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003200" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215432L1 | Lenti ORF clone of Human transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 1, Myc-DDK-tagged | 10 ug |
$909.00
|
|
| RC215432L2 | Lenti ORF clone of Human transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 1, mGFP tagged | 10 ug |
$909.00
|
|
| RC215432L3 | Lenti ORF clone of Human transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 1, Myc-DDK-tagged | 10 ug |
$909.00
|
|
| RC215432L4 | Lenti ORF clone of Human transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 1, mGFP tagged | 10 ug |
$909.00
|
|
| RG215432 | TCF3 (tGFP-tagged) - Human transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 1 | 10 ug |
$489.00
$809.00
|
|
| SC303283 | TCF3 (untagged)-Human transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 1 | 10 ug |
$610.00
|
|
Citations
IRE1α-XBP1 safeguards hematopoietic stem and progenitor cells by restricting pro-leukemogenic gene programs
Barton, Son, Verma et al
Nat Immunol (2025) 26 (2), 200-214 DOI: 10.1038/s41590-024-02063-w
Barton, Son, Verma et al
Nat Immunol (2025) 26 (2), 200-214 DOI: 10.1038/s41590-024-02063-w
Cited by 17
AI Summary: In this study, OriGene’s full-length human TCF3 plasmid (RC215432) was used to investigate the molecular mechanisms underlying IRE1α-XBP1 signaling in hematopoietic stem and progenitor cells.
Snippet: CF4 (Addgene, 16512);pcDNA3-S33Y β-catenin (Addgene, 19286);p178 Grg-1L/TLE1 (Addgene, 11067);and human β-catenin GFP (Addgene, 71367). Plasmids encoding full-length human TCF3 (Origene, RC215432) and LEF1 (Sino Biological, HG12090) were purchased commercially. Full-length human TCF1 and TCF3 coding sequences were PCR amplified to incorporate a Myc tag on th
T cell factor 4 is a pro-catabolic and apoptotic factor in human articular chondrocytes by potentiating nuclear factor κB signaling
Ma, Zhong, van Blitterswijk et al
J. Biol. Chem. (2013) 288 (24), 17552-8 DOI: 10.1074/jbc.M113.453985
Ma, Zhong, van Blitterswijk et al
J. Biol. Chem. (2013) 288 (24), 17552-8 DOI: 10.1074/jbc.M113.453985
AI Summary: OriGene's human TCF3 variant 1 (E12) and variant 2 (E47) ORF sequences were used to generate lentiviral constructs for TCF3 overexpression, enabling the study to assess the specific roles of TCF/LEF transcription factors in human chondrocytes.
Snippet: Protein or RNA samples were harvested 72 h after adenoviral transduction unless otherwise specified. Human TCF3 variant1 (E12) and variant 2 (E47) ORF sequences (Origene) were cloned in to the lentiviral vector pBOB (Addgene plasmid 12335) (11).
Showing 1-2 of 2 papers.
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