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PHACTR2 (NM_014721) Human Tagged ORF Clone

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SKU
RC215421
PHACTR2 (Myc-DDK-tagged)-Human phosphatase and actin regulator 2 (PHACTR2), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $590.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PHACTR2
Synonyms C6orf56
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215421 representing NM_014721
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACAACGCCGTTGACGGACTGGACAAAGCTTCTATAGCAAACTCAGATGGCCCCACAGCAGGTTCCC
AAACACCTCCCTTCAAAAGAAAGGGGAAACTATCCACCATTGGTAAAATCTTTAAGCCTTGGAAATGGAG
GAAAAAGAAGACCAGCGACAAATTTAGAGAAACCTCGGCAGTATTAGAAAGGAAGATATCCACACGACAA
AGTAGAGAGGAGCTGATAAGAAGGGGAGTGCTTAAGGAATTGCCTGATCAAGATGGAGATGTAACAGTTA
ACTTTGAAAATTCAAACGGGCACATGATACCCATCGGAGAGGAATCTACCCGAGAGGAAAATGTAGTAAA
GTCTGAAGAAGGTAATGGCTCTGTATCTGAAAAAACACCACCTCTGGAGGAACAGGCAGAAGATAAGAAA
GAGAACACTGAAAACCACTCTGAAACACCGGCAGCTCCTGCTCTACCTCCTTCTGCTCCTCCTAAGCCTA
GACCCAAACCTAAACCCAAAAAATCACCTGTGCCTCCGAAAGGGGCCACTGCTGGGGCCAGCCACAAAGG
TGATGAAGTGCCTCCCATTAAAAAAAATACCAAGGCTCCTGGTAAGCAGGCCCCCGTCCCTCCACCCAAG
CCAGCAAGCCGAAACACGACCCGAGAGGCTGCTGGCTCCTCTCATTCAAAAAAAACAACTGGCTCTAAAG
CATCAGCTTCGCCATCCACTTCATCCACCTCATCTCGTCCCAAAGCTTCAAAGGAGACAGTTTCTAGCAA
AGCAGGGACAGTGGGGACCACCAAGGGCAAGAGAAAAACTGACAAGCAGCCAATAACTTCTCACCTGTCC
TCAGACACAACAACTTCTGGCACATCCGACCTGAAAGGAGAGCCTGCAGAGACCAGAGTGGAGAGTTTCA
AACTCGAACAGACTGTCCCTGGAGCTGAGGAGCAGAACACAGGCAAATTCAAGTCCATGGTCCCTCCACC
CCCTGTGGCTCCAGCACCTTCTCCTCTGGCCCCCCCTCTCCCTCTTGAGGATCAGTGCATTACTGCCTCA
GACACTCCAGTTGTCCTCGTCAGCGTTGGAGCTGACCTGCCCGTCTCTGCCTTAGACCCAAGTCAGCTTC
TTTGGGCTGAAGAGCCGACGAACAGAACCACTCTCTACTCAGGCACTGGCTTAAGTGTTAACAGAGAAAA
TGCAAAATGTTTCACAACCAAAGAGGAGCTGGGGAAGACAGTGCCTCAGCTACTGACTCCTGGGCTGATG
GGCGAATCTTCAGAATCCTTTAGTGCCTCAGAAGATGAAGGCCACAGGGAATACCAAGCCAATGACTCTG
ACTCGGACGGGCCTATCTTGTACACCGATGATGAGGACGAAGACGAAGATGAGGATGGCAGTGGAGAAAG
TGCTTTGGCAAGTAAAATACGCCGGAGGGATACTCTTGCTATCAAACTTGGCAACAGACCATCTAAGAAA
GAACTAGAGGACAAAAACATCTTGCAGCGTACATCTGAAGAAGAGAGGCAGGAAATCCGACAACAAATTG
GAACCAAGTTAGTCAGGAGGCTGAGCCAGAGGCCCACAACTGAAGAATTAGAGCAAAGAAACATCCTAAA
ACAAAAGAATGAAGAAGAGGAACAAGAAGCAAAAATGGAACTTAAACGCAGACTCAGCAGAAAGCTCAGC
CTGAGACCCACAGTGGCAGAGCTACAAGCTCGAAGGATCCTGCGATTTAACGAGTATGTAGAAGTCACGG
ATTCTCCTGACTATGACCGCCGAGCAGACAAGCCCTGGGCCAGGTTAACACCTGCAGACAAGGCAGCAAT
AAGGAAAGAACTAAATGAATTTAAAAGCACAGAAATGGAAGTTCATGAAGAGAGTCGACAGTTTACAAGG
TTTCATCGTCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215421 representing NM_014721
Red=Cloning site Green=Tags(s)

MDNAVDGLDKASIANSDGPTAGSQTPPFKRKGKLSTIGKIFKPWKWRKKKTSDKFRETSAVLERKISTRQ
SREELIRRGVLKELPDQDGDVTVNFENSNGHMIPIGEESTREENVVKSEEGNGSVSEKTPPLEEQAEDKK
ENTENHSETPAAPALPPSAPPKPRPKPKPKKSPVPPKGATAGASHKGDEVPPIKKNTKAPGKQAPVPPPK
PASRNTTREAAGSSHSKKTTGSKASASPSTSSTSSRPKASKETVSSKAGTVGTTKGKRKTDKQPITSHLS
SDTTTSGTSDLKGEPAETRVESFKLEQTVPGAEEQNTGKFKSMVPPPPVAPAPSPLAPPLPLEDQCITAS
DTPVVLVSVGADLPVSALDPSQLLWAEEPTNRTTLYSGTGLSVNRENAKCFTTKEELGKTVPQLLTPGLM
GESSESFSASEDEGHREYQANDSDSDGPILYTDDEDEDEDEDGSGESALASKIRRRDTLAIKLGNRPSKK
ELEDKNILQRTSEEERQEIRQQIGTKLVRRLSQRPTTEELEQRNILKQKNEEEEQEAKMELKRRLSRKLS
LRPTVAELQARRILRFNEYVEVTDSPDYDRRADKPWARLTPADKAAIRKELNEFKSTEMEVHEESRQFTR
FHRP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014721
ORF Size 1902 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014721.3
RefSeq Size 4318 bp
RefSeq ORF 1905 bp
Locus ID 9749
UniProt ID O75167
Cytogenetics 6q24.2
Domains RPEL
MW 69.5 kDa
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Documents
Resources
"NM_014721" in other vectors (4)
SKU Description Size Price
RC215421L3 Lenti-ORF clone of PHACTR2 (Myc-DDK-tagged)-Human phosphatase and actin regulator 2 (PHACTR2), transcript variant 3 10 ug
$890.00
RC215421L4 Lenti-ORF clone of PHACTR2 (mGFP-tagged)-Human phosphatase and actin regulator 2 (PHACTR2), transcript variant 3 10 ug
$890.00
RG215421 PHACTR2 (tGFP-tagged) - Human phosphatase and actin regulator 2 (PHACTR2), transcript variant 3 10 ug
$790.00
SC314280 PHACTR2 (untagged)-Human phosphatase and actin regulator 2 (PHACTR2), transcript variant 3 10 ug
$650.00

Plasmid Map for RC215421

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215421
Circular map for RC215421

file-alt Citations

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