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RASGRF1 (NM_153815) Human Tagged ORF Clone

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SKU
RC215382
RASGRF1 (Myc-DDK-tagged)-Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RASGRF1
Synonyms CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55; PP13187; ras-GRF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215382 representing NM_153815
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACTCGGCCATGTCACCCTTCAGCAAGGCCACGCTGGACACCAGCAAGCTCTATGTGTCCAGCAGCT
TCACCAACAAGATTCCAGATGAGGGCGATACGACCCCTGAGAAGCCCGAAGACCCTTCAGCGCTCAGCAA
GCAGAGCTCAGAAGTCTCCATGAGAGAGGAGTCAGATATTGATCAAAACCAGAGTGATGATGGTGATACT
GAAACATCACCAACTAAATCTCCAACAACACCCAAATCAGTCAAAAACAAAAATTCTTCAGAGTTCCCAC
TCTTTTCCTATAACAATGGAGTCGTCATGACCTCCTGTCGTGAACTGGACAATAACCGCAGTGCCTTGTC
GGCCGCCTCTGCCTTTGCCATAGCAACCGCCGGGGCCAACGAGGGCACCCCAAACAAGGAGAAGTACCGG
AGGATGTCCTTAGCCAGTGCAGGGTTTCCCCCAGACCAGAGGAATGGAGACAAGGAGTTTGTGATCCGCA
GAGCAGCCACCAATCGTGTCTTGAACGTGCTCCGCCACTGGGTGTCCAAGCACTCTCAGGACTTTGAGAC
CAACGATGAGCTCAAATGCAAGGTGATCGGCTTCCTGGAAGAAGTCATGCACGACCCGGAGCTCCTGACC
CAGGAGCGGAAGGCTGCAGCCAACATCATCAGGACTCTGACCCAGGAGGACCCAGGTGACAACCAGATCA
CGCTGGAGGAGATCACGCAGATGGCTGAAGGCGTGAAGGCTGAGCCCTTTGAAAACCACTCAGCCCTGGA
GATCGCGGAGCAGCTGACCCTGCTAGATCACCTCGTCTTCAAGAAGATTCCTTATGAGGAGTTCTTCGGA
CAAGGATGGATGAAACTGGAAAAGAATGAAAGGACCCCTTATATCATGAAAACCACTAAGCACTTCAATG
ACATCAGTAACTTGATTGCTTCAGAAATCATCCGCAATGAGGACATCAACGCCAGGGTGAGCGCCATCGA
GAAGTGGGTGGCCGTAGCTGACATATGCCGCTGCCTCCACAACTACAATGCCGTACTGGAGATCACCTCG
TCCATGAACCGCAGTGCAATCTTCCGGCTCAAAAAGACGTGGCTCAAAGTCTCTAAGCAGACTAAAGCTT
TGATTGATAAGCTCCAAAAGCTTGTGTCATCTGAGGGCAGATTTAAGAATCTCAGAGAAGCTCTGAAAAA
TTGTGACCCACCCTGTGTCCCTTACCTGGGGATGTACCTCACCGACCTGGCCTTCATCGAGGAGGGGACG
CCCAATTACACGGAAGACGGCCTGGTCAACTTCTCCAAGATGAGGATGATATCCCATATTATCCGAGAGA
TTCGCCAGTTTCAACAAACTGCCTACAAAATAGAGCACCAAGCAAAGGTAACGCAATATTTACTGGACCA
ATCTTTTGTAATGGATGAAGAAAGCCTCTACGAGTCTTCTCTCCGAATAGAACCAAAACTCCCCACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215382 representing NM_153815
Red=Cloning site Green=Tags(s)

MYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDT
ETSPTKSPTTPKSVKNKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYR
RMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLT
QERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFG
QGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITS
SMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGT
PNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153815
ORF Size 1467 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153815.2, NP_722522.1
RefSeq Size 2843 bp
RefSeq ORF 1470 bp
Locus ID 5923
UniProt ID Q13972, Q13972-2
Cytogenetics 15q25.1
Domains RasGEF
Protein Families Druggable Genome
Protein Pathways Focal adhesion, MAPK signaling pathway
MW 55.3 kDa
Summary The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. provided by RefSeq, Mar 2009
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Documents
Resources
"NM_153815" in other vectors (4)
SKU Description Size Price
RC215382L3 Lenti ORF clone of Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC215382L4 Lenti ORF clone of Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 2, mGFP tagged 10 ug
$757.00
RG215382 RASGRF1 (tGFP-tagged) - Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 2 10 ug
$657.00
SC310336 RASGRF1 (untagged)-Human Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 2 10 ug
$503.00

Plasmid Map for RC215382

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215382
Circular map for RC215382

file-alt Citations

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