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CHFR (NM_018223) Human Tagged ORF Clone

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SKU
RC215377
CHFR (Myc-DDK-tagged)-Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $580.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CHFR
Synonyms RNF116; RNF196
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215377 representing NM_018223
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCCCTGGGGACGGCTCCTGCGTCTGGGCG
CGGAGGAGGGCGAGCCGCACGTCCTCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGA
CCTTTCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGTGGATGAAAAATCAGGT
CAGGTGACACTGGAAGATACCAGCACCAGTGGAACAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGA
CATGCCCTTTACAGACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAACGTGGC
ATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAGAATCCTTTGAGATGGTGCCTTGCTGT
GTTGCCCAGGCTGGTCTAAAACTCCTGGGATCAAGTGATCCTCCCACCTTGGCCTCCCAAAGTATTGTGA
TTACAGGGTCTGGGGGTGGTGGCATCTCCCCTAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTC
CAGCTTTGCCTCAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAGGATCAGGAG
GATTTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTGTTGGTCG
CACAACCGCGTAGAAATGCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGAT
GGAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGTGAGTTTGCAGCCCTGCATG
CACACGTTCTGCGCGGCTTGCTACTCGGGCTGGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTC
CCGTGGAGCGGATCTGTAAAAACCACATCCTCAACAACCTCGTGGAAGCATACCTCATCCAGCATCCAGA
CAAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGATGCCAGGAATAAAATCACTCAAGACATGCTGCAG
CCCAAAGTCAGGCGGTCTTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTTG
ACAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTCCTGAGTACAGAAGGCAGGC
GGCGCAGCCTCCCCACTGCCCAGCACCCGAGGGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCACCC
TCCACGTCCGTCAGCCTGACGACAGCAGTCCAGGATTACGTGTGCCCTCTGCAAGGAAGCCACGCCCTGT
GCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGCGGAGCGCGAGCAGGACCCGCGTGTCGCCCCTCA
GCAGTGTGCGGTCTGCCTGCAGCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGC
TGCCTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGCGTGCTGAACAACAACAGCT
ACGAGTCAGACATCCTGAAGAATTACCTGGCAACCAGAGGTTTGACATGGAAAAACATGTTGACCGAGAG
CCTCGTGGCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACCGTTCTGTGT
TACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCAGTATCGGCAGAACATTCCTGCTTCCGAGT
TGCCAGTGGCCGTAACATCCCGTCCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCA
CCACGCCATGAAATTCAATCATATCTGTGAACAGACAAGGTTCAAAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215377 representing NM_018223
Red=Cloning site Green=Tags(s)

MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSG
QVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFEMVPCC
VAQAGLKLLGSSDPPTLASQSIVITGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQE
DLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCM
HTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQ
PKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAP
STSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYG
CLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLC
YCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018223
ORF Size 1869 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018223.2, NP_060693.2
RefSeq Size 3138 bp
RefSeq ORF 1872 bp
Locus ID 55743
UniProt ID Q96EP1
Cytogenetics 12q24.33
Domains FHA, RING
Protein Families Druggable Genome
MW 69 kDa
Summary This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. provided by RefSeq, Mar 2014
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"NM_018223" in other vectors (4)
SKU Description Size Price
RC215377L3 Lenti-ORF clone of CHFR (Myc-DDK-tagged)-Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4 10 ug
$880.00
RC215377L4 Lenti-ORF clone of CHFR (mGFP-tagged)-Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4 10 ug
$880.00
RG215377 CHFR (tGFP-tagged) - Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4 10 ug
$780.00
SC314239 CHFR (untagged)-Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4 10 ug
$639.00

Plasmid Map for RC215377

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215377
Circular map for RC215377

file-alt Citations

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