KRTAP2-4 (NM_033184) Human Tagged ORF Clone

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SKU
RC215356
KRTAP2 (Myc-DDK-tagged)-Human keratin associated protein 2-4 (KRTAP2-4)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRTAP2-4
Synonyms KAP2.1B; KAP2.3; KAP2.4; KRTAP2-3; KRTAP2.3; KRTAP2.4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215356 representing NM_033184
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGGCTCCTGCTGCGGCTCCACCTTGTCCTCCCTGAGCTACGGGGGAGGCTGCTGCCAGCCCTGCT
GCTGCCGCGACCCCTGCTGCTGCCGCCCCGTGACCTGCCAGACCACCGTGTGCCGCCCCGTGACCTGCGT
GCCCCGCTGCACGCGCCCCATCTGCGAGCCCTGCCGCCGCCCGGTGTGCTGCGACCCCTGCTCCCTGCAG
GAAGGCTGCTGCCGCCCCATCACCTGCTGCCCCTCGTCGTGCACGGCTGTGGTGTGCAGGCCCTGCTGCT
GGGCCACCACCTGCTGCCAGCCTGTGTCTGTGCAGTCCCCCTGCTGCCGGCCTCCCTGCGGCCAGCCGAC
CCCTTGCAGCACCACCTGCAGGACCTCCTCCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215356 representing NM_033184
Red=Cloning site Green=Tags(s)

MTGSCCGSTLSSLSYGGGCCQPCCCRDPCCCRPVTCQTTVCRPVTCVPRCTRPICEPCRRPVCCDPCSLQ
EGCCRPITCCPSSCTAVVCRPCCWATTCCQPVSVQSPCCRPPCGQPTPCSTTCRTSSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033184
ORF Size 384 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033184.2, NP_149440.1
RefSeq Size 812 bp
RefSeq ORF 387 bp
Locus ID 85294
UniProt ID P0C7H8, Q9BYR9
Cytogenetics 17q21.2
MW 13.3 kDa
Summary This protein is a member of the keratin-associated protein (KAP) family. The KAP proteins form a matrix of keratin intermediate filaments which contribute to the structure of hair fibers. KAP family members appear to have unique, family-specific amino- and carboxyl-terminal regions and are subdivided into three multi-gene families according to amino acid composition: the high sulfur, the ultrahigh sulfur, and the high tyrosine/glycine KAPs. This protein is a member of the high sulfur KAP family and the gene is localized to a cluster of KAPs at 17q12-q21. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_033184" in other vectors (6)
SKU Description Size Price
RC215356L1 Lenti ORF clone of Human keratin associated protein 2-4 (KRTAP2-4), Myc-DDK-tagged 10 ug
$450.00
RC215356L2 Lenti ORF clone of Human keratin associated protein 2-4 (KRTAP2-4), mGFP tagged 10 ug
$450.00
RC215356L3 Lenti ORF clone of Human keratin associated protein 2-4 (KRTAP2-4), Myc-DDK-tagged 10 ug
$450.00
RC215356L4 Lenti ORF clone of Human keratin associated protein 2-4 (KRTAP2-4), mGFP tagged 10 ug
$450.00
RG215356 KRTAP2 (tGFP-tagged) - Human keratin associated protein 2-4 (KRTAP2-4) 10 ug
$489.00
SC123080 KRTAP2 (untagged)-Human keratin associated protein 2-4 (KRTAP2-4) 10 ug
$150.00

Plasmid Map for RC215356

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Cloning scheme for RC215356

Circular map for RC215356

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