MMP28 (NM_024302) Human Tagged ORF Clone
- TrueORF®
SKU
RC215325
MMP28 (Myc-DDK-tagged)-Human matrix metallopeptidase 28 (MMP28), transcript variant 1
$774.00
2 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MMP28 |
| Synonyms | EPILYSIN; MM28; MMP-25; MMP-28; MMP25 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_024302 |
| ORF Size | 1560 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_024302.5 |
| RefSeq Size | 2480 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 79148 |
| UniProt ID | Q9H239 |
| Cytogenetics | 17q12 |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| MW | 56.4 kDa |
| Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction and tissue remodeling, and disease processes, such as asthma and metastasis. This gene encodes a secreted enzyme that degrades casein. Its expression pattern suggests that it plays a role in tissue homeostasis and in wound repair. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Apr 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_024302" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215325L1 | Lenti-ORF clone of MMP28 (Myc-DDK-tagged)-Human matrix metallopeptidase 28 (MMP28), transcript variant 1 | 10 ug |
$1,074.00
|
|
| RC215325L2 | Lenti-ORF clone of MMP28 (mGFP-tagged)-Human matrix metallopeptidase 28 (MMP28), transcript variant 1 | 10 ug |
$1,074.00
|
|
| RC215325L3 | Lenti-ORF clone of MMP28 (Myc-DDK-tagged)-Human matrix metallopeptidase 28 (MMP28), transcript variant 1 | 10 ug |
$1,074.00
|
|
| RC215325L4 | Lenti-ORF clone of MMP28 (mGFP-tagged)-Human matrix metallopeptidase 28 (MMP28), transcript variant 1 | 10 ug |
$1,074.00
|
|
| RG215325 | MMP28 (tGFP-tagged) - Human matrix metallopeptidase 28 (MMP28), transcript variant 1 | 10 ug |
$974.00
|
|
| SC125588 | MMP28 (untagged)-Human matrix metallopeptidase 28 (MMP28), transcript variant 1 | 10 ug |
$727.00
|
|
Citations
Upregulation and Nuclear Location of MMP28 in Alveolar Epithelium of Idiopathic Pulmonary Fibrosis
Maldonado, Salgado-Aguayo, Herrera et al
Am. J. Respir. Cell Mol. Biol. (2018)
Maldonado, Salgado-Aguayo, Herrera et al
Am. J. Respir. Cell Mol. Biol. (2018)
AI Summary: OriGene products, including the pCMV6-Entry vector (PS100001) and MMP28-DDK expression plasmid (RC215325), were used to overexpress wild-type and mutant forms of MMP28 in alveolar epithelial cells, enabling the study of MMP28’s role in cell proliferation, migration, and epithelial-mesenchymal transition in idiopathic pulmonary fibrosis.
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
