APLP1 (NM_005166) Human Tagged ORF Clone
SKU
RC215282
APLP1 (Myc-DDK-tagged)-Human amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant 2
$605.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | APLP1 |
| Synonyms | APLP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005166 |
| ORF Size | 1950 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005166.4 |
| RefSeq Size | 2447 bp |
| RefSeq ORF | 1953 bp |
| Locus ID | 333 |
| UniProt ID | P51693 |
| Cytogenetics | 19q13.12 |
| Domains | A4_EXTRA |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 72.18 kDa |
| Summary | This gene encodes a member of the highly conserved amyloid precursor protein gene family. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005166" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215282L1 | Lenti ORF clone of Human amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$905.00
|
|
| RC215282L2 | Lenti ORF clone of Human amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant 2, mGFP tagged | 10 ug |
$905.00
|
|
| RC215282L3 | Lenti ORF clone of Human amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$905.00
|
|
| RC215282L4 | Lenti ORF clone of Human amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant 2, mGFP tagged | 10 ug |
$905.00
|
|
| RG215282 | APLP1 (tGFP-tagged) - Human amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant 2 | 10 ug |
$489.00
$805.00
|
|
| SC116888 | APLP1 (untagged)-Human amyloid beta (A4) precursor-like protein 1 (APLP1), transcript variant 2 | 10 ug |
$606.00
|
|
Citations
Functional Divergence and Origin of the Vertebrate Praja Family
Onodera, Kawasaki, Oishi et al
J Mol Evol (2023)
Onodera, Kawasaki, Oishi et al
J Mol Evol (2023)
Cited by 4
AI Summary: OriGene’s pCMV6-Entry plasmid containing APLP1 cDNA (OriGene #RC215282) was used as a vector backbone for constructing expression plasmids in the study investigating the functional divergence and evolutionary origin of the vertebrate Praja family.
Snippet: Plasmid vectors were assembled using Gibson assembly (NEB #E5520S). Initially, APLP1 was truncated from pCMV6-Entry (ORIGENE #RC215282) containing APLP1 cDNA, followed by subcloning of praja1 (Promega #FXC13949E, identical to NP_001369704.1) or praja2 (Promega # FXC01596, with Arg replacing Q297 of NP_055634.3)
APLP1 and APLP2, members of the app family of proteins, behave similarly to app in that they associate with nmda receptors and enhance nmda receptor surface expression
Cousins, Dai, Stephenson
J. Neurochem. (2015)
Cousins, Dai, Stephenson
J. Neurochem. (2015)
Cited by 31
Showing 1-2 of 2 papers.
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