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SAMD4A (NM_015589) Human Tagged ORF Clone

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SKU
RC215243
SAMD4A (Myc-DDK-tagged)-Human sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $657.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SAMD4A
Synonyms SAMD4; SMAUG; SMAUG1; SMG; SMGA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215243 representing NM_015589
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCGCGACCAGGTCGGGGTGCTGGCGGGCTGGTTTAAGGGCTGGAACGAGTGCGAGCAGACTGTTG
CGCTGCTGTCGCTGCTCAAGCGCGTGAGCCAGACCCAGGCCCGCTTCCTCCAGCTCTGCCTGGAGCACTC
GCTGGCCGACTGCGCCGAGCTGCACGTCCTCGAACGCGAGGCCAACAGCCCCGGAATCATTAACCAATGG
CAACAGGAATCCAAGGATAAAGTGATTTCCCTCCTGTTAACTCATCTGCCTTTGCTGAAGCCAGGAAACC
TCGACGCGAAAGTAGAATATATGAAACTGCTGCCCAAAATCCTGGCTCACTCTATTGAACACAACCAGCA
CATTGAGGAGAGCAGGCAGCTGCTGTCCTATGCTTTGATACATCCAGCCACTTCGTTAGAAGACCGTAGT
GCTTTAGCCATGTGGCTGAATCACTTGGAGGACCGCACGTCGACCAGCTTTGGTGGCCAGAACCGAGGCC
GCTCAGACTCTGTGGATTATGGACAGACACACTACTATCACCAAAGACAGAACTCTGATGACAAGCTCAA
TGGGTGGCAGAACTCTCGGGATTCTGGGATTTGCATCAATGCCTCCAACTGGCAGGACAAAAGCATGGGG
TGTGAGAATGGCCATGTGCCCCTCTACTCCTCCTCATCTGTCCCCACCACAATCAATACGATTGGAACCA
GCACAAGTACAATTCTCTCAGGCCAGGCACACCACAGCCCTTTGAAACGATCTGTGTCCCTTACCCCACC
CATGAATGTGCCAAACCAGCCTCTAGGACATGGATGGATGTCTCATGAGGACTTACGAGCTAGAGGACCC
CAGTGCCTCCCATCCGATCATGCCCCCCTGTCTCCACAAAGCAGTGTAGCCTCTTCAGGAAGTGGTGGGA
GTGAACACTTAGAAGATCAGACCACTGCTCGTAACACATTCCAAGAAGAAGGTAGTGGGATGAAAGATGT
TCCAGCCTGGCTGAAAAGCCTCCGCCTGCACAAATATGCCGCGCTTTTCTCCCAGATGACCTATGAGGAG
ATGATGGCCCTCACCGAGTGCCAGCTGGAGGCGCAGAATGTTACCAAAGGTGCAAGACACAAAATTGTCA
TCAGTATTCAGAAGCTCAAAGAAAGACAAAATCTCCTGAAGTCTTTGGAAAGGGACATCATCGAGGGGGG
CAGCCTGCGCATCCCGCTCCAGGAACTGCACCAGATGATCCTGACTCCGATCAAGGCCTACAGCTCCCCG
AGCACCACCCCCGAGGCTCGCCGCCGGGAGCCCCAGGCCCCGCGTCAGCCCTCACTGATGGGCCCCGAGA
GCCAGAGCCCCGACTGCAAAGATGGGGCCGCAGCCACTGGCGCCACGGCCACCCCCTCGGCCGGGGCCAG
CGGGGGGCTCCAGCCGCACCAGCTGAGCAGCTGCGATGGGGAGCTGGCCGTCGCCCCCCTGCCAGAGGGG
GACCTCCCCGGGCAGTTCACACGCGTCATGGGGAAAGTGTGCACACAGCTCTTGGTCTCCAGACCTGATG
AGGAAAATATAAGTTCCTATTTACAGCTCATAGACAAGTGTCTAATTCATGAGGCATTTACAGAGACACA
GAAAAAAAGATTGTTGTCATGGAAACAGCAGGTGCAGAAGCTCTTTCGGTCTTTCCCTCGGAAAACCCTT
CTAGACATATCAGGATATCGACAGCAAAGAAATCGAGGCTTTGGGCAATCCAACTCCCTCCCGACGGCTG
GCTCTGTGGGCGGTGGCATGGGCAGACGGAACCCGCGCCAGTACCAGATCCCCTCTCGGAACGTCCCTTC
CGCCCGCCTGGGCCTCTTGGGCACCAGTGGATTCGTCAGCTCCAACCAGCGCAACACCACAGCTACCCCC
ACCATCATGAAACAAGGAAGACAGAACCTGTGGTTTGCCAACCCCGGGGGCAGCAATAGCATGCCAAGCC
GCACCCACAGCTCAGTCCAGAGGACCCGCTCGCTGCCCGTGCACACTTCCCCACAGAACATGCTGATGTT
CCAGCAGCCAGAATTCCAGCTTCCCGTGACCGAACCTGACATCAACAACAGGCTGGAGTCGTTGTGCCTC
AGTATGACCGAACACGCCCTGGGAGACGGGGTTGACCGGACCTCCACCATC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215243 representing NM_015589
Red=Cloning site Green=Tags(s)

MFRDQVGVLAGWFKGWNECEQTVALLSLLKRVSQTQARFLQLCLEHSLADCAELHVLEREANSPGIINQW
QQESKDKVISLLLTHLPLLKPGNLDAKVEYMKLLPKILAHSIEHNQHIEESRQLLSYALIHPATSLEDRS
ALAMWLNHLEDRTSTSFGGQNRGRSDSVDYGQTHYYHQRQNSDDKLNGWQNSRDSGICINASNWQDKSMG
CENGHVPLYSSSSVPTTINTIGTSTSTILSGQAHHSPLKRSVSLTPPMNVPNQPLGHGWMSHEDLRARGP
QCLPSDHAPLSPQSSVASSGSGGSEHLEDQTTARNTFQEEGSGMKDVPAWLKSLRLHKYAALFSQMTYEE
MMALTECQLEAQNVTKGARHKIVISIQKLKERQNLLKSLERDIIEGGSLRIPLQELHQMILTPIKAYSSP
STTPEARRREPQAPRQPSLMGPESQSPDCKDGAAATGATATPSAGASGGLQPHQLSSCDGELAVAPLPEG
DLPGQFTRVMGKVCTQLLVSRPDEENISSYLQLIDKCLIHEAFTETQKKRLLSWKQQVQKLFRSFPRKTL
LDISGYRQQRNRGFGQSNSLPTAGSVGGGMGRRNPRQYQIPSRNVPSARLGLLGTSGFVSSNQRNTTATP
TIMKQGRQNLWFANPGGSNSMPSRTHSSVQRTRSLPVHTSPQNMLMFQQPEFQLPVTEPDINNRLESLCL
SMTEHALGDGVDRTSTI

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_015589
ORF Size 2151 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015589.4
RefSeq Size 2356 bp
RefSeq ORF 2157 bp
Locus ID 23034
UniProt ID Q9UPU9
Cytogenetics 14q22.2
MW 79.1 kDa
Summary Sterile alpha motifs (SAMs) in proteins such as SAMD4A are part of an RNA-binding domain that functions as a posttranscriptional regulator by binding to an RNA sequence motif known as the Smaug recognition element, which was named after the Drosophila Smaug protein (Baez and Boccaccio, 2005 PubMed 16221671).supplied by OMIM, Mar 2008
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Documents
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"NM_015589" in other vectors (4)
SKU Description Size Price
RC215243L3 Lenti ORF clone of Human sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1, Myc-DDK-tagged 10 ug
$957.00
RC215243L4 Lenti ORF clone of Human sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1, mGFP tagged 10 ug
$957.00
RG215243 SAMD4A (tGFP-tagged) - Human sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1 10 ug
$857.00
SC304332 SAMD4A (untagged)-Human sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1 10 ug
$724.00

Plasmid Map for RC215243

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215243
Circular map for RC215243

file-alt Citations

A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis
Amadei, Zander, Yang et al
J. Neurosci. (2015) 35 (47), 15666-81 DOI: 10.1523/JNEUROSCI.2172-15.2015
AI Summary: OriGene products contributed to this study by providing Flag-tagged expression constructs for mouse and human Smaug2 and Smaug1, as well as mouse Nanos1, Nanos2, Nanos3, and human Nanos1, which were used to investigate the roles of these proteins in neurogenesis.
Snippet: different mother. Plasmids and shRNAs. The Flag-tagged expression constructs for mouse and human Smaug2 and Smaug1 and mouse Nanos1, Nanos 2, and Nanos3 and human Nanos1 were obtained from OriGene. shRNA ... Cite SaveSaving
Showing 1-1 of 1 papers.
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