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GPRASP2 (NM_138437) Human Tagged ORF Clone

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SKU
RC215168
GPRASP2 (Myc-DDK-tagged)-Human G protein-coupled receptor associated sorting protein 2 (GPRASP2), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $767.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPRASP2
Synonyms DFNX7; GASP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215168 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGGGGCAGAGATTGAGCCTAGTGCCCAGGCCAAGCCTGAAAAGAAGGCTGGGGAAGAGGTTATCG
CTGGGCCTGAGAGAGAGAATGATGTCCCTCTGGTGGTCAGACCCAAGGTTAGGACCCAGGCAACTACTGG
GGCAAGGCCCAAAACTGAGACCAAGTCTGTGCCTGCGGCAAGGCCCAAAACTGAGGCCCAAGCAATGTCT
GGGGCAAGGCCCAAAACTGAGGTCCAAGTAATGGGTGGTGCAAGACCCAAAACGGAGGCTCAAGGAATCA
CAGGGGCCAGGCCCAAAACCGATGCCAGGGCAGTAGGTGGCGCTCGTTCTAAAACTGATGCCAAGGCAAT
CCCTGGAGCAAGGCCCAAGGATGAGGCCCAGGCATGGGCCCAGAGTGAATTTGGGACTGAAGCAGTGTCA
CAGGCAGAAGGAGTGTCCCAGACTAATGCCGTTGCTTGGCCACTGGCCACTGCTGAGTCTGGATCAGTTA
CTAAATCTAAGGGCCTGTCTATGGATAGAGAACTAGTCAATGTGGATGCTGAAACCTTTCCTGGCACCCA
GGGTCAGAAAGGAATCCAGCCCTGGTTTGGACCAGGGGAGGAGACTAATATGGGGTCTTGGTGCTATTCC
AGGCCCAGGGCCAGAGAGGAGGCCTCTAATGAGTCTGGGTTCTGGTCAGCAGATGAGACCTCTACAGCGT
CTTCTTTCTGGACTGGAGAAGAGACAAGTGTCAGATCATGGCCCAGGGAAGAGTCCAATACCAGGTCCAG
GCACAGGGCTAAACATCAGACTAATCCCAGGTCCAGGCCCAGATCCAAGCAAGAAGCCTATGTTGATTCC
TGGTCTGGATCTGAGGATGAGGCCAGCAACCCATTCTCCTTCTGGGTTGGAGAAAATACCAATAACTTGT
TCAGGCCCAGAGTCAGGGAGGAGGCAAATATCAGGTCCAAGCTCAGGACAAATAGAGAAGATTGTTTTGA
ATCTGAGTCTGAAGATGAGTTCTATAAGCAGTCCTGGGTTTTGCCTGGAGAAGAGGCCAATAGTAGATTC
AGGCACAGAGACAAAGAAGATCCTAATACTGCCTTGAAACTCAGGGCCCAGAAAGATGTTGACAGTGATA
GGGTCAAACAAGAACCCAGGTTTGAGGAGGAAGTCATTATTGGGTCCTGGTTCTGGGCAGAAAAAGAGGC
CAGTTTGGAGGGTGGAGCTTCAGCAATCTGTGAATCTGAGCCAGGAACTGAGGAGGGGGCCATTGGCGGA
TCCGCGTACTGGGCTGAGGAAAAGTCCAGTTTGGGGGCTGTGGCCAGAGAAGAGGCCAAGCCGGAGTCTG
AAGAAGAGGCCATATTTGGGTCCTGGTTCTGGGACAGAGATGAGGCCTGCTTTGACCTAAATCCCTGTCC
TGTGTACAAGGTCAGTGATAGGTTCAGAGATGCAGCTGAGGAGCTTAATGCATCCTCCAGGCCCCAAACC
TGGGACGAGGTCACTGTTGAATTCAAACCTGGTCTTTTTCATGGGGTTGGCTTCCGATCCACAAGCCCCT
TTGGAATTCCCGAAGAGGCTTCTGAAATGCTTGAGGCAAAGCCCAAGAACCTGGAACTTAGCCCAGAAGG
AGAAGAGCAGGAATCTTTGCTTCAGCCTGATCAGCCTAGTCCTGAGTTCACATTTCAGTATGATCCTTCC
TACCGGTCAGTCCGGGAAATTCGAGAGCATCTTAGGGCCAGGGAGAGTGCAGAGTCTGAGAGTTGGTCAT
GCAGCTGCATACAATGTGAGCTGAAAATTGGTTCTGAAGAGTTTGAAGAATTCCTTTTATTAATGGACAA
AATTCGGGATCCTTTTATTCATGAAATATCTAAAATTGCAATGGGTATGAGAAGTGCTTCTCAATTTACC
CGAGATTTCATTCGAGATTCAGGTGTTGTCTCACTTATTGAAACCTTGCTTAATTATCCATCCTCTAGAG
TTAGGACAAGTTTTTTGGAAAATATGATTCACATGGCTCCACCTTATCCAAATCTAAACATGATTGAGAC
ATTCATATGTCAAGTGTGTGAGGAAACCCTTGCACATAGTGTGGATTCCCTTGAGCAGCTGACTGGAATA
AGGATGCTTAGACACCTCACTATGACTATTGACTATCACACACTGATTGCCAACTATATGTCCGGGTTTC
TCTCCTTATTAACCACAGCCAATGCGAGAACGAAGTTTCACGTTCTGAAAATGCTATTGAATTTGTCTGA
AAATCCTGCTGTGGCAAAAAAACTATTCAGTGCCAAAGCTCTTTCAATATTTGTGGGTCTCTTTAACATA
GAAGAGACAAATGATAATATTCAAATTGTTATTAAAATGTTTCAGAATATCAGTAACATTATAAAAAGTG
GAAAGATGTCCTTAATTGATGATGATTTCAGTCTTGAGCCGCTTATTTCTGCATTTCGTGAATTTGAGGA
GTTAGCTAAGCAACTACAAGCCCAAATAGACAACCAAAATGATCCTGAGGTGGGACAACAAAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215168 protein sequence
Red=Cloning site Green=Tags(s)

MTGAEIEPSAQAKPEKKAGEEVIAGPERENDVPLVVRPKVRTQATTGARPKTETKSVPAARPKTEAQAMS
GARPKTEVQVMGGARPKTEAQGITGARPKTDARAVGGARSKTDAKAIPGARPKDEAQAWAQSEFGTEAVS
QAEGVSQTNAVAWPLATAESGSVTKSKGLSMDRELVNVDAETFPGTQGQKGIQPWFGPGEETNMGSWCYS
RPRAREEASNESGFWSADETSTASSFWTGEETSVRSWPREESNTRSRHRAKHQTNPRSRPRSKQEAYVDS
WSGSEDEASNPFSFWVGENTNNLFRPRVREEANIRSKLRTNREDCFESESEDEFYKQSWVLPGEEANSRF
RHRDKEDPNTALKLRAQKDVDSDRVKQEPRFEEEVIIGSWFWAEKEASLEGGASAICESEPGTEEGAIGG
SAYWAEEKSSLGAVAREEAKPESEEEAIFGSWFWDRDEACFDLNPCPVYKVSDRFRDAAEELNASSRPQT
WDEVTVEFKPGLFHGVGFRSTSPFGIPEEASEMLEAKPKNLELSPEGEEQESLLQPDQPSPEFTFQYDPS
YRSVREIREHLRARESAESESWSCSCIQCELKIGSEEFEEFLLLMDKIRDPFIHEISKIAMGMRSASQFT
RDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIHMAPPYPNLNMIETFICQVCEETLAHSVDSLEQLTGI
RMLRHLTMTIDYHTLIANYMSGFLSLLTTANARTKFHVLKMLLNLSENPAVAKKLFSAKALSIFVGLFNI
EETNDNIQIVIKMFQNISNIIKSGKMSLIDDDFSLEPLISAFREFEELAKQLQAQIDNQNDPEVGQQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138437
ORF Size 2514 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138437.6
RefSeq Size 3730 bp
RefSeq ORF 2517 bp
Locus ID 114928
UniProt ID Q96D09, B3KW05
Cytogenetics Xq22.1
Domains DUF634
Protein Families Druggable Genome
MW 93.8 kDa
Summary The protein encoded by this gene is a member of a family that regulates the activity of G protein-coupled receptors (GPCRs). The encoded protein has been shown to be capable of interacting with several GPCRs, including the M1 muscarinic acetylcholine receptor and the calcitonin receptor. Several transcript variants encoding the same protein have been found for this gene. provided by RefSeq, May 2010
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Documents
Resources
"NM_138437" in other vectors (4)
SKU Description Size Price
RC215168L3 Lenti ORF clone of Human G protein-coupled receptor associated sorting protein 2 (GPRASP2), transcript variant 2, Myc-DDK-tagged 10 ug
$1,067.00
RC215168L4 Lenti ORF clone of Human G protein-coupled receptor associated sorting protein 2 (GPRASP2), transcript variant 2, mGFP tagged 10 ug
$1,067.00
RG215168 GPRASP2 (tGFP-tagged) - Human G protein-coupled receptor associated sorting protein 2 (GPRASP2), transcript variant 2 10 ug
$789.00 $967.00
SC120460 GPRASP2 (untagged)-Human G protein-coupled receptor associated sorting protein 2 (GPRASP2), transcript variant 2 10 ug
$768.00

Plasmid Map for RC215168

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215168
Circular map for RC215168

file-alt Citations

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