BMP8A (NM_181809) Human Tagged ORF Clone

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SKU
RC215047
BMP8A (Myc-DDK-tagged)-Human bone morphogenetic protein 8a (BMP8A)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BMP8A
Synonyms OP-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215047 representing NM_181809
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCGCGCCCCGGACCGCTCTGGCTTCTGGGCCTGACGTTGTGCGCGCTGGGCGGGGGCGGCCCCG
GCCTGCGACCCCCGCCCGGCTGTCCCCAGCGACGTCTGGGCGCGCGCGAGCGCCGGGACGTGCAGCGCGA
GATCCTGGCGGTGCTCGGGCTACCCGGGCGGCCCCGGCCCCGCGCGCCACCCGCCGCCTCCCGGCTGCCC
GCGTCCGCGCCGCTCTTCATGCTGGACCTGTACCACGCCATGGCTGGCGACGACGACGAGGACGGCGCGC
CCGCGGAGCAGCGCCTGGGCCGCGCCGACCTGGTCATGAGCTTCGTCAACATGGTGGAGCGAGACCGTGC
CCTGGGCCACCAGGAGCCCCATTGGAAGGAGTTCCGCTTTGACCTGACCCAGATCCCGGCTGGGGAGGCG
GTCACAGCTGCGGAGTTCCGGATTTACAAGGTGCCCAGCATCCACCTGCTCAACAGGACCCTCCACGTCA
GCATGTTCCAGGTGGTCCAGGAGCAGTCCAACAGGGAGTCTGACTTGTTCTTTTTGGATCTTCAGACGCT
CCGAGCTGGAGACGAGGGCTGGCTGGTGCTGGATGTCACAGCAGCCAGTGACTGCTGGTTGCTGAAGCGT
CACAAGGACCTGGGACTCCGCCTCTATGTGGAGACTGAGGATGGGCACAGCGTGGATCCTGGCCTGGCCG
GCCTGCTGGGTCAACGGGCCCCACGCTCCCAACAGCCTTTCGTGGTCACTTTCTTCAGGGCCAGTCCGAG
TCCCATCCGCACCCCTCGGGCAGTGAGGCCACTGAGGAGGAGGCAGCCGAAGAAAACCAACGAGCTGCCG
CAGGCCAACCGACTCCCAGGGATCTTTGATGACGTCCACGGCTCCCACGGCCGGCAGGTCTGCCGTCGGC
ACGAGCTCTACGTCAGCTTCCAGGACCTTGGCTGGCTGGACTGGGTCATCGCCCCCCAAGGCTACTCAGC
CTATTACTGTGAGGGGGAGTGCTCCTTCCCGCTGGACTCCTGCATGAACGCCACCAACCACGCCATCCTG
CAGTCCCTGGTGCACCTGATGAAGCCAAACGCAGTCCCCAAGGCGTGCTGTGCACCCACCAAGCTGAGCG
CCACCTCTGTGCTCTACTATGACAGCAGCAACAACGTCATCCTGCGCAAGCACCGCAACATGGTGGTCAA
GGCCTGCGGCTGCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215047 representing NM_181809
Red=Cloning site Green=Tags(s)

MAARPGPLWLLGLTLCALGGGGPGLRPPPGCPQRRLGARERRDVQREILAVLGLPGRPRPRAPPAASRLP
ASAPLFMLDLYHAMAGDDDEDGAPAEQRLGRADLVMSFVNMVERDRALGHQEPHWKEFRFDLTQIPAGEA
VTAAEFRIYKVPSIHLLNRTLHVSMFQVVQEQSNRESDLFFLDLQTLRAGDEGWLVLDVTAASDCWLLKR
HKDLGLRLYVETEDGHSVDPGLAGLLGQRAPRSQQPFVVTFFRASPSPIRTPRAVRPLRRRQPKKTNELP
QANRLPGIFDDVHGSHGRQVCRRHELYVSFQDLGWLDWVIAPQGYSAYYCEGECSFPLDSCMNATNHAIL
QSLVHLMKPNAVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181809
ORF Size 1206 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181809.4
RefSeq Size 1692 bp
RefSeq ORF 1209 bp
Locus ID 353500
UniProt ID Q7Z5Y6
Cytogenetics 1p34.3
Protein Families Secreted Protein
Protein Pathways Hedgehog signaling pathway, TGF-beta signaling pathway
MW 44.6 kDa
Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in development of the reproductive system. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 1. provided by RefSeq, Jul 2016
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Documents
Resources
"NM_181809" in other vectors (4)
SKU Description Size Price
RC215047L3 Lenti ORF clone of Human bone morphogenetic protein 8a (BMP8A), Myc-DDK-tagged 10 ug
$757.00
RC215047L4 Lenti ORF clone of Human bone morphogenetic protein 8a (BMP8A), mGFP tagged 10 ug
$757.00
RG215047 BMP8A (tGFP-tagged) - Human bone morphogenetic protein 8a (BMP8A) 10 ug
$657.00
SC307379 BMP8A (untagged)-Human bone morphogenetic protein 8a (BMP8A) 10 ug
$457.00

Plasmid Map for RC215047

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Cloning scheme for RC215047

Circular map for RC215047

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