DNAJC12 (NM_201262) Human Tagged ORF Clone

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SKU
RC215035
DNAJC12 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 2
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DNAJC12
Synonyms HPANBH4; JDP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215035 representing NM_201262
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCAATACTGAATTACAGGTCAGAAGATACTGAAGATTACTACACATTACTGGGATGTGATGAAC
TATCTTCGGTTGAACAAATCCTGGCAGAATTTAAAGTCAGAGCTCTGGAATGTCACCCAGACAAGCATCC
TGAAAACCCCAAAGCTGTGGAGACTTTTCAGAAACTGCAGAAGGCAAAGGAGATTCTGACCAATGAAGAG
AGTCGAGCCCGCTATGACCACTGGCGAAGGAGCCAGATGTCGATGCCATTCCAGCAGTGGGAAGCTTTGA
ATGACTCAGTGAAGACGGTGGGTTTCTCGCTGGGTGCGACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215035 representing NM_201262
Red=Cloning site Green=Tags(s)

MDAILNYRSEDTEDYYTLLGCDELSSVEQILAEFKVRALECHPDKHPENPKAVETFQKLQKAKEILTNEE
SRARYDHWRRSQMSMPFQQWEALNDSVKTVGFSLGAT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_201262
ORF Size 321 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201262.1, NP_957714.1
RefSeq Size 786 bp
RefSeq ORF 324 bp
Locus ID 56521
UniProt ID Q9UKB3
Cytogenetics 10q21.3
MW 12.3 kDa
Summary This gene encodes a member of a subclass of the HSP40/DnaJ protein family. Members of this family of proteins are associated with complex assembly, protein folding, and export. Two transcript variants encoding distinct isoforms have been identified for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_201262" in other vectors (4)
SKU Description Size Price
RC215035L3 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC215035L4 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 2, mGFP tagged 10 ug
$450.00
RG215035 DNAJC12 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 2 10 ug
$350.00
SC308000 DNAJC12 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 2 10 ug
$165.00

Plasmid Map for RC215035

Download sequence for

Cloning scheme for RC215035

Circular map for RC215035

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