DMAC2 (NM_018035) Human Tagged ORF Clone

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SKU
RC214916
ATP5SL (Myc-DDK-tagged)-Human ATP5S-like (ATP5SL), transcript variant 4
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DMAC2
Synonyms ATP5SL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214916 representing NM_018035
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTCCCTGGGCGTCCCTGCGCCTGGTCGCCCCCATGTGGAATGGGCGTATCAGGGGCATCCATC
GCCTGGGTGCGGCAGTGGCCCCAGAGGGCAGTCAGAAGAAGAAAAGGACAATACTCCAGTTCCTGACCAA
CTATTTCTACGATGTGGAGGCTCTGAGGGATTACTTGCTCCAAAGGGAGATGTACAAGGTGCATGAGAAA
AATCGATCTTACACCTGGCTGGAGAAGCAACATGGTCCATACGGCGCAGGTGCCTTTTTCATCCTGAAGC
AGGGAGGCGCAGTCAAGTTTCGAGACAAGGAGTGGATCAGGCCAGATAAGTATGGCCATTTCTCTCAGGA
GTTCTGGAATTTCTGTGAAGTGCCTGTCGAAGCTGTGGATGCCGGTGACTGTGACATCAACTACGAGGGC
CTGGATAACCTCCTCCGCCTGAAGGAGCTCCAGTCCTTGTCGCTGCAGCGCTGCTCCCACGTGGACGACT
GGTGTCTCAGCCGCCTCTACCCACTGGCCGACTCGTTGCAGGAGCTCTCGCTGGCCGGTTGCCCCCGCAT
CTCCGAACGGGGCCTCGCCTGCCTCCACCACCTCCAGAACCTCCGCAGGCTGGACATCTCGGACCTCCCT
GCCGTGTCCAACCCTGGCCTCACTCAGATATTGGTGGAGGAGATGCTGCCCAATTGCGAGGTTGTGGGAG
TCGACTGGGCTGAGGGCCTGAAGTCAGGGCCGGAGGAGCAGCCTCGGGACACAGCCAGCCCTGTCCCTGC
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214916 representing NM_018035
Red=Cloning site Green=Tags(s)

MAAPWASLRLVAPMWNGRIRGIHRLGAAVAPEGSQKKKRTILQFLTNYFYDVEALRDYLLQREMYKVHEK
NRSYTWLEKQHGPYGAGAFFILKQGGAVKFRDKEWIRPDKYGHFSQEFWNFCEVPVEAVDAGDCDINYEG
LDNLLRLKELQSLSLQRCSHVDDWCLSRLYPLADSLQELSLAGCPRISERGLACLHHLQNLRRLDISDLP
AVSNPGLTQILVEEMLPNCEVVGVDWAEGLKSGPEEQPRDTASPVPA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018035
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018035.1, NP_060505.1
RefSeq Size 1692 bp
RefSeq ORF 774 bp
Locus ID 55101
UniProt ID Q9NW81, A0A024R0K4
Cytogenetics 19q13.2
MW 29.1 kDa
Summary Required for the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Involved in the assembly of the distal region of complex I.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_018035" in other vectors (4)
SKU Description Size Price
RC214916L3 Lenti ORF clone of Human ATP5S-like (ATP5SL), transcript variant 4, Myc-DDK-tagged 10 ug
$600.00
RC214916L4 Lenti ORF clone of Human ATP5S-like (ATP5SL), transcript variant 4, mGFP tagged 10 ug
$600.00
RG214916 ATP5SL (tGFP-tagged) - Human ATP5S-like (ATP5SL), transcript variant 4 10 ug
$489.00 $500.00
SC113819 ATP5SL (untagged)-Human ATP5S-like (ATP5SL), transcript variant 4 10 ug
$300.00

Plasmid Map for RC214916

Download sequence for

Cloning scheme for RC214916

Circular map for RC214916

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