CRISPLD2 (NM_031476) Human Tagged ORF Clone
SKU
RC214912
CRISPLD2 (Myc-DDK-tagged)-Human cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2)
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CRISPLD2 |
| Synonyms | CRISP11; LCRISP2; LGL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_031476 |
| ORF Size | 1491 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_031476.4 |
| RefSeq Size | 4574 bp |
| RefSeq ORF | 1494 bp |
| Locus ID | 83716 |
| UniProt ID | Q9H0B8, A0A140VK80 |
| Cytogenetics | 16q24.1 |
| Domains | LCCL, SCP |
| Protein Families | Secreted Protein, Transmembrane |
| MW | 55.7 kDa |
| Summary | Promotes matrix assembly.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_031476" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214912L1 | Lenti ORF clone of Human cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC214912L2 | Lenti ORF clone of Human cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2), mGFP tagged | 10 ug |
$986.00
|
|
| RC214912L3 | Lenti ORF clone of Human cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC214912L4 | Lenti ORF clone of Human cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2), mGFP tagged | 10 ug |
$986.00
|
|
| RG214912 | CRISPLD2 (tGFP-tagged) - Human cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2) | 10 ug |
$886.00
|
|
| SC108001 | CRISPLD2 (untagged)-Human cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2) | 10 ug |
$686.00
|
|
Citations
Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells
Wosczyna, Perez Carbajal, Wagner et al
Cell Stem Cell (2021) 28 (7), 1323-1334.e8 DOI: 10.1016/j.stem.2021.04.008
Wosczyna, Perez Carbajal, Wagner et al
Cell Stem Cell (2021) 28 (7), 1323-1334.e8 DOI: 10.1016/j.stem.2021.04.008
Cited by 88
AI Summary: In this study, OriGene’s Runx1 expression plasmid (RC206307) and Runx1 shRNA plasmid (TL513525) were used to manipulate Runx1 levels in mesenchymal stromal cells, enabling the authors to investigate the role of Runx1 in adipogenic differentiation regulated by miR-206.
Crispld2 is required for neural crest cell migration and cell viability during zebrafish craniofacial development
Swindell, Yuan, Maili et al
Genesis (2015)
Swindell, Yuan, Maili et al
Genesis (2015)
Cited by 23
Showing 1-2 of 2 papers.
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