ARID1B (NM_020732) Human Tagged ORF Clone
- TrueORF®
SKU
RC214830
ARID1B (Myc-DDK-tagged)-Human AT rich interactive domain 1B (SWI1-like) (ARID1B), transcript variant 2
$2,784.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ARID1B |
| Synonyms | 6A3-5; BAF250B; BRIGHT; CSS1; DAN15; ELD/OSA1; MRD12; OSA2; P250R |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_020732 |
| ORF Size | 6747 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq Size | 8126 bp |
| RefSeq ORF | 6750 bp |
| Locus ID | 57492 |
| UniProt ID | Q8NFD5 |
| Cytogenetics | 6q25.3 |
| Domains | ARID |
| MW | 237.7 kDa |
| Summary | This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_020732" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214830L1 | Lenti ORF clone of Human AT rich interactive domain 1B (SWI1-like) (ARID1B), transcript variant 2, Myc-DDK-tagged | 10 ug |
$3,084.00
|
|
| RG214830 | ARID1B (tGFP-tagged) - Human AT rich interactive domain 1B (SWI1-like) (ARID1B), transcript variant 2 | 10 ug |
$2,984.00
|
|
| SC309795 | ARID1B (untagged)-Human AT rich interactive domain 1B (SWI1-like) (ARID1B), transcript variant 2 | 10 ug |
$1,990.00
|
|
Citations
De Novo ARID1B Mutations Cause Growth Delay Associated with Aberrant Wnt/β-Catenin Signaling
Liu, Hu, Ye et al
Hum. Mutat. (2020)
Liu, Hu, Ye et al
Hum. Mutat. (2020)
Cited by 29
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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