EIF4H (NM_022170) Human Tagged ORF Clone

  • TrueORF®
SKU
RC214822
EIF4H (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4H (EIF4H), transcript variant 1
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EIF4H
Synonyms eIF-4H; WBSCR1; WSCR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214822 representing NM_022170
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACTTCGACACCTACGACGATCGGGCCTACAGCAGCTTCGGCGGCGGCAGAGGGTCCCGCGGCA
GTGCTGGTGGCCATGGTTCCCGTAGCCAGAAGGAGTTGCCCACAGAGCCCCCCTACACAGCATACGTAGG
AAATCTACCTTTCAATACGGTTCAGGGCGACATAGATGCTATCTTTAAGGATCTCAGCATAAGGAGTGTA
CGGCTAGTCAGAGACAAAGACACAGATAAATTTAAAGGATTCTGCTATGTAGAATTCGATGAAGTGGATT
CCCTTAAGGAAGCCTTGACATACGATGGTGCACTGTTGGGCGATCGGTCACTTCGTGTGGACATTGCAGA
AGGCAGAAAACAAGATAAAGGTGGCTTTGGATTCAGAAAAGGTGGACCAGATGACAGAGGAATGGGTAGC
TCTCGAGAATCTAGAGGTGGATGGGATTCCCGGGATGACTTCAATTCTGGCTTCAGGGATGACTTCTTAG
GGGGCAGGGGAGGTAGTCGCCCAGGCGACCGGCGAACAGGCCCCCCCATGGGCAGCCGCTTCAGAGATGG
CCCTCCCCTCCGTGGATCCAACATGGATTTCAGAGAACCCACAGAAGAGGAAAGAGCACAGAGACCACGA
CTCCAGCTTAAACCTCGAACAGTCGCGACGCCCCTCAATCAAGTAGCCAATCCCAACTCTGCTATCTTCG
GGGGTGCCAGGCCTAGAGAGGAAGTCGTTCAAAAGGAGCAAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214822 representing NM_022170
Red=Cloning site Green=Tags(s)

MADFDTYDDRAYSSFGGGRGSRGSAGGHGSRSQKELPTEPPYTAYVGNLPFNTVQGDIDAIFKDLSIRSV
RLVRDKDTDKFKGFCYVEFDEVDSLKEALTYDGALLGDRSLRVDIAEGRKQDKGGFGFRKGGPDDRGMGS
SRESRGGWDSRDDFNSGFRDDFLGGRGGSRPGDRRTGPPMGSRFRDGPPLRGSNMDFREPTEEERAQRPR
LQLKPRTVATPLNQVANPNSAIFGGARPREEVVQKEQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022170
ORF Size 744 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022170.2
RefSeq Size 2546 bp
RefSeq ORF 747 bp
Locus ID 7458
UniProt ID Q15056
Cytogenetics 7q11.23
Domains RRM
MW 27.2 kDa
Summary This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_022170" in other vectors (4)
SKU Description Size Price
RC214822L3 Lenti-ORF clone of EIF4H (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4H (EIF4H), transcript variant 1 10 ug
$750.00
RC214822L4 Lenti-ORF clone of EIF4H (mGFP-tagged)-Human eukaryotic translation initiation factor 4H (EIF4H), transcript variant 1 10 ug
$750.00
RG214822 EIF4H (tGFP-tagged) - Human eukaryotic translation initiation factor 4H (EIF4H), transcript variant 1 10 ug
$650.00
SC127305 EIF4H (untagged)-Human eukaryotic translation initiation factor 4H (EIF4H), transcript variant 1 10 ug
$300.00

Plasmid Map for RC214822

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Cloning scheme for RC214822

Circular map for RC214822

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