CRMP3 (DPYSL4) (NM_006426) Human Tagged ORF Clone
$532.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CRMP3 |
| Synonyms | CRMP3; DRP-4; ULIP4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006426 |
| ORF Size | 1716 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006426.3 |
| RefSeq Size | 2699 bp |
| RefSeq ORF | 1719 bp |
| Locus ID | 10570 |
| UniProt ID | O14531 |
| Cytogenetics | 10q26.3 |
| Domains | Amidohydro_1 |
| MW | 61.9 kDa |
| Summary | Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006426" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214821L3 | Lenti ORF clone of Human dihydropyrimidinase-like 4 (DPYSL4), Myc-DDK-tagged | 10 ug |
$832.00
|
|
| RC214821L4 | Lenti ORF clone of Human dihydropyrimidinase-like 4 (DPYSL4), mGFP tagged | 10 ug |
$832.00
|
|
| RG214821 | DPYSL4 (tGFP-tagged) - Human dihydropyrimidinase-like 4 (DPYSL4) | 10 ug |
$489.00
$732.00
|
|
| SC116112 | DPYSL4 (untagged)-Human dihydropyrimidinase-like 4 (DPYSL4) | 10 ug |
$533.00
|
|
Citations
Cdk5 and GSK3β inhibit Fast Endophilin-Mediated Endocytosis
Ferreira, Casamento, Roas et al
bioRxiv (2020)
Ferreira, Casamento, Roas et al
bioRxiv (2020)
Cited by 3
Snippet: Page 1. 1 Cdk5 and GSK3β inhibit Fast Endophilin-Mediated Endocytosis 1 2 3 Antonio PA Ferreira1,2,* , Alessandra Casamento1,* , Sara Carrillo Roas1, James Panambalana1, Shaan 4 Subramaniam1,8, Kira Schutzenhofer1;Full length and truncated genes (all human, unless specified) were amplified and cloned into pDONR201 (Invitrogen) and 500 transferred into pEGFP, pTagRFP-T (called ‘RFP’ elsewhere), pMyc or pGEX-6P2 vectors converted into the Gateway 501 system (pDEST vectors made from a pCI backbone), as appropriate: Endophilin-A2 (SH3GL1, IMAGE 3458016) full 502 length and SH3 domain (aa 311-end);Endophilin-A1 (SH3GL2 iso1, FLJ 92732) full length and SH3 domain (aa 295- 503 end);Endophilin-A3 (SH3GL3 iso 1, IMAGE 5197246) full length and SH3 domain (aa 291-end);Bin1, also known as 504 Amphiphysin-II (BIN1 iso9, cloned from human brain cDNA library) full length;full length CRMP2 (DPYSL2, DNASU 505 HsCD00513405), full length CRMP3 (DPYSL4, NM_006426 Origene), full length mouse CRMP4 (DPYSL3, Origene 506 1197294), full length CRMP5 (DPYSL5, amplified from human brain cDNA library, Novagen), Ephrin receptor A1 507 cytoplasmic tail (aa 568-976) (EPHA1, DNASU HsCD00516390), Ephrin receptor A6 cytoplasmic tail (aa 572-1036) 508 (EPHA6, DNASU HsCD00350501), Ephrin receptor B1 cytoplasmic tail (aa 259-346) (EPHB1, DNASU HsCD00038738), 509 Ephrin receptor B4 cytoplasmic tail (aa 561-987) (EFNB4, DNASU HsCD00021508), Ephrin receptor B6 cytoplasmic tail 510 (aa 616-1021) (EPHB6, DNASU HsCD00505529), Semaphorin 4F cytoplasmic tail (aa 681-770) (SEMA4F, DNASU
Showing 1-1 of 1 papers.
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