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SPINK4 (NM_014471) Human Tagged ORF Clone

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SKU
RC214818
SPINK4 (Myc-DDK-tagged)-Human serine peptidase inhibitor, Kazal type 4 (SPINK4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPINK4
Synonyms HEL136; PEC-60; PEC60
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214818 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGTCCGCCAGTGGGTAATCGCCCTGGCCTTGGCTGCCCTCCTTGTTGTGGACAGGGAAGTGCCAG
TGGCAGCAGGAAAGCTCCCTTTCTCAAGAATGCCCATCTGTGAACACATGGTAGAGTCTCCAACCTGTTC
CCAGATGTCCAACCTGGTCTGCGGCACTGATGGGCTCACATATACGAATGAATGCCAGCTCTGCTTGGCC
CGGATAAAAACCAAACAGGACATCCAGATCATGAAAGATGGCAAATGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214818 protein sequence
Red=Cloning site Green=Tags(s)

MAVRQWVIALALAALLVVDREVPVAAGKLPFSRMPICEHMVESPTCSQMSNLVCGTDGLTYTNECQLCLA
RIKTKQDIQIMKDGKC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014471
ORF Size 258 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014471.3
RefSeq Size 386 bp
RefSeq ORF 261 bp
Locus ID 27290
UniProt ID O60575, V9HWG8
Cytogenetics 9p13.3
Protein Families Secreted Protein
MW 9.5 kDa
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"NM_014471" in other vectors (4)
SKU Description Size Price
RC214818L3 Lenti ORF clone of Human serine peptidase inhibitor, Kazal type 4 (SPINK4), Myc-DDK-tagged 10 ug
$450.00
RC214818L4 Lenti ORF clone of Human serine peptidase inhibitor, Kazal type 4 (SPINK4), mGFP tagged 10 ug
$450.00
RG214818 SPINK4 (tGFP-tagged) - Human serine peptidase inhibitor, Kazal type 4 (SPINK4) 10 ug
$489.00
SC304114 SPINK4 (untagged)-Human serine peptidase inhibitor, Kazal type 4 (SPINK4) 10 ug
$165.00

Plasmid Map for RC214818

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214818
Circular map for RC214818

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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