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ARS2 (SRRT) (NM_015908) Human Tagged ORF Clone

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SKU
RC214814
SRRT (Myc-DDK-tagged)-Human serrate RNA effector molecule homolog (Arabidopsis) (SRRT), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $802.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ARS2
Synonyms ARS2; ASR2; serrate
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214814 representing NM_015908
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGACAGTGATGACGAGTACGATCGAAGGCGCAGGGACAAGTTCAGAAGAGAGCGCAGCGACTACG
ACCGTTCCCGCGAGAGAGATGAAAGACGTCGAGGGGACGATTGGAATGACAGAGAGTGGGACCGTGGCCG
TGAGCGCCGTAGTCGGGGTGAATATCGGGACTATGACCGGAATCGGCGAGAGCGCTTCTCGCCACCTCGC
CACGAACTCAGCCCGCCACAGAAGCGCATGAGGAGAGACTGGGATGAGCACAGCTCTGACCCATACCACA
GTGGCTATGAGATGCCCTATGCTGGGGGGGGTGGGGGCCCAACTTATGGCCCCCCTCAGCCCTGGGGCCA
CCCTGACGTCCACATCATGCAGCACCATGTCCTGCCTATCCAGGCCAGGCTGGGCAGCATTGCAGAGATT
GACCTGGGTGTGCCGCCGCCCGTGATGAAGACCTTCAAGGAGTTTCTCCTCTCCCTGGATGACTCGGTGG
ATGAGACGGAGGCCGTCAAGCGCTATAATGACTACAAGCTGGATTTCCGGAGGCAACAGATGCAGGATTT
CTTCCTGGCGCACAAAGATGAGGAGTGGTTTCGGTCTAAGTACCACCCAGATGAGGTGGGGAAGCGTCGG
CAGGAGGCCCGGGGGGCCCTGCAAAACCGACTGAGGGTCTTCCTGTCCCTCATGGAGACTGGCTGGTTTG
ATAACCTTCTCCTGGACATAGACAAAGCTGATGCCATTGTCAAGATGCTGGATGCAGCCGTGATTAAGAT
GGAAGGAGGCACGGAGAATGATCTTCGCATCCTGGAGCAGGAGGAGGAGGAGGAGCAGGCAGGAAAGCCT
GGGGAGCCCAGCAAGAAAGAAGAAGGACGGGCTGGAGCAGGCCTAGGGGACGGGGAGCGCAAAACCAACG
ACAAGGATGAGAAGAAGGAAGACGGCAAGCAGGCTGAGAATGACAGTTCTAATGATGACAAAACAAAGAA
GTCGGAGGGTGATGGGGACAAGGAAGAGAAGAAAGAAGACTCCGAGAAGGAAGCCAAAAAGAGTAGCAAG
AAGCGGAACCGGAAGCACAGTGGTGACGACAGCTTTGACGAGGGCAGCGTGTCAGAGTCTGAGTCGGAGT
CAGAGAGCGGCCAGGCTGAGGAGGAGAAGGAGGAGGCCGAAGAAGCGCTCAAGGAGAAGGAGAAGCCCAA
GGAAGAAGAATGGGAGAAGCCCAAGGACGCCGCGGGGCTGGAGTGCAAGCCGCGGCCGCTGCATAAGACC
TGCTCCCTCTTCATGCGCAACATCGCGCCCAACATCTCCCGGGCCGAGATCATCTCCCTTTGTAAAAGGT
ACCCAGGCTTTATGCGGGTGGCGCTCTCAGAGCCCCAGCCAGAGAGGAGGTTTTTCCGTCGTGGCTGGGT
GACCTTCGACCGCAGTGTTAACATTAAAGAGATCTGTTGGAACCTGCAGAACATCCGTCTCCGGGAGTGT
GAGCTGAGCCCTGGTGTGAACAGGGACCTGACCCGGCGCGTTCGCAACATCAACGGCATCACCCAGCACA
AGCAGATTGTGCGCAACGACATCAAGCTGGCGGCCAAGCTGATCCACACGCTGGATGACAGGACACAGCT
TTGGGCCTCAGAACCAGGGACGCCTCCCCTGCCCACGAGCCTGCCCTCGCAAAACCCGATCTTGAAGAAT
ATCACCGACTACCTGATCGAGGAAGTAAGCGCCGAGGAGGAGGAGCTGCTGGGGAGCAGCGGGGGCGCTC
CTCCTGAGGAGCCTCCTAAGGAAGGGAACCCGGCAGAGATCAACGTGGAGCGGGATGAGAAGTTGATTAA
GGTCTTGGACAAGCTCCTCCTTTACCTGCGCATCGTGCATTCCTTGGATTATTACAACACCTGTGAGTAC
CCCAACGAGGACGAGATGCCCAATCGCTGTGGGATCATCCACGTTCGGGGGCCCATGCCACCCAACCGCA
TCAGTCACGGGGAAGTGCTGGAGTGGCAGAAGACTTTTGAGGAGAAGCTCACGCCGTTGCTGAGTGTGCG
GGAGTCACTCTCAGAGGAAGAGGCCCAGAAGATGGGGCGCAAAGACCCAGAGCAGGAAGTGGAGAAGTTC
GTCACCTCCAACACGCAGGAACTGGGCAAGGATAAGTGGCTGTGTCCTCTCAGTGGCAAGAAATTCAAGG
GTCCTGAGTTTGTGCGCAAACATATCTTCAACAAGCATGCAGAGAAAATTGAGGAAGTGAAAAAGGAAGT
CGCGTTTTTTAACAACTTCCTCACTGATGCTAAGCGCCCAGCTCTGCCTGAGATCAAGCCAGCCCAGCCA
CCTGGCCCCGCCCAGATACTCCCCCCAGGTTTGACCCCAGGACTCCCCTACCCACACCAGACTCCCCAGG
GCCTGATGCCCTATGGTCAGCCCCGGCCCCCGATCTTGGGCTATGGAGCTGGTGCTGTCCGCCCTGCAGT
CCCCACAGGAGGCCCTCCATACCCCCATGCCCCGTATGGTGCTGGTCGAGGGAACTATGATGCCTTCCGA
GGCCAGGGAGGTTATCCTGGGAAACCTCGCAACAGGATGGTTCGTGGAGACCCAAGGGCCATTGTGGAAT
ATCGGGACCTGGATGCCCCAGACGATGTTGATTTCTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214814 representing NM_015908
Red=Cloning site Green=Tags(s)

MGDSDDEYDRRRRDKFRRERSDYDRSRERDERRRGDDWNDREWDRGRERRSRGEYRDYDRNRRERFSPPR
HELSPPQKRMRRDWDEHSSDPYHSGYEMPYAGGGGGPTYGPPQPWGHPDVHIMQHHVLPIQARLGSIAEI
DLGVPPPVMKTFKEFLLSLDDSVDETEAVKRYNDYKLDFRRQQMQDFFLAHKDEEWFRSKYHPDEVGKRR
QEARGALQNRLRVFLSLMETGWFDNLLLDIDKADAIVKMLDAAVIKMEGGTENDLRILEQEEEEEQAGKP
GEPSKKEEGRAGAGLGDGERKTNDKDEKKEDGKQAENDSSNDDKTKKSEGDGDKEEKKEDSEKEAKKSSK
KRNRKHSGDDSFDEGSVSESESESESGQAEEEKEEAEEALKEKEKPKEEEWEKPKDAAGLECKPRPLHKT
CSLFMRNIAPNISRAEIISLCKRYPGFMRVALSEPQPERRFFRRGWVTFDRSVNIKEICWNLQNIRLREC
ELSPGVNRDLTRRVRNINGITQHKQIVRNDIKLAAKLIHTLDDRTQLWASEPGTPPLPTSLPSQNPILKN
ITDYLIEEVSAEEEELLGSSGGAPPEEPPKEGNPAEINVERDEKLIKVLDKLLLYLRIVHSLDYYNTCEY
PNEDEMPNRCGIIHVRGPMPPNRISHGEVLEWQKTFEEKLTPLLSVRESLSEEEAQKMGRKDPEQEVEKF
VTSNTQELGKDKWLCPLSGKKFKGPEFVRKHIFNKHAEKIEEVKKEVAFFNNFLTDAKRPALPEIKPAQP
PGPAQILPPGLTPGLPYPHQTPQGLMPYGQPRPPILGYGAGAVRPAVPTGGPPYPHAPYGAGRGNYDAFR
GQGGYPGKPRNRMVRGDPRAIVEYRDLDAPDDVDFF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015908
ORF Size 2628 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015908.6
RefSeq Size 2994 bp
RefSeq ORF 2631 bp
Locus ID 51593
UniProt ID Q9BXP5
Cytogenetics 7q22.1
MW 100.5 kDa
Summary Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Independently of its activity on miRNAs, necessary and sufficient to promote neural stem cell self-renewal. Does so by directly binding SOX2 promoter and positively regulating its transcription (By similarity).UniProtKB/Swiss-Prot Function
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"NM_015908" in other vectors (5)
SKU Description Size Price
RC214814L3 Lenti ORF clone of Human serrate RNA effector molecule homolog (Arabidopsis) (SRRT), transcript variant 1, Myc-DDK-tagged 10 ug
$1,102.00
RC214814L4 Lenti ORF clone of Human serrate RNA effector molecule homolog (Arabidopsis) (SRRT), transcript variant 1, mGFP tagged 10 ug
$1,102.00
RG214814 SRRT (tGFP-tagged) - Human serrate RNA effector molecule homolog (Arabidopsis) (SRRT), transcript variant 1 10 ug
$1,002.00
SC114586 SRRT (untagged)-Human serrate RNA effector molecule homolog (Arabidopsis) (SRRT), transcript variant 1 10 ug
$457.00
SC318351 SRRT (untagged)-Human serrate RNA effector molecule homolog (Arabidopsis) (SRRT), transcript variant 1 10 ug
$883.00

Plasmid Map for RC214814

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214814
Circular map for RC214814

file-alt Citations

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