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CDH20 (NM_031891) Human Tagged ORF Clone

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SKU
RC214769
CDH20 (Myc-DDK-tagged)-Human cadherin 20, type 2 (CDH20)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $733.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDH20
Synonyms Cdh7; CDH7L3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214769 representing NM_031891
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGACTTCTGGTAGAATGAGCAATGCAAAGAACTGGCTTGGACTTGGCATGTCCTTGTACTTCTGGG
GGCTGATGGACCTTACGACCACCGTTCTCTCGGACACCCCAACACCACAAGGTGAATTAGAAGCACTCCT
GTCAGACAAGCCACAGTCACATCAGCGGACCAAGAGGAGCTGGGTTTGGAACCAGTTTTTCGTTCTGGAA
GAGTACACTGGGACCGACCCTTTGTATGTCGGCAAGCTTCATTCAGATATGGACAGGGGAGACGGATCCA
TCAAATACATCCTCTCGGGAGAAGGTGCTGGCATCGTGTTTACCATCGACGACACCACTGGAGACATCCA
CGCCATTCAGAGGCTCGATCGAGAGGAAAGAGCCCAGTATACTCTAAGGGCTCAAGCCCTAGACAGGCGG
ACGGGCAGGCCAATGGAGCCCGAGTCAGAGTTCATCATCAAAATTCAAGACATCAATGACAATGAGCCCA
AGTTCCTGGACGGACCTTATGTGGCCACTGTGCCAGAAATGTCCCCTGTGGGTACCTCCGTCATCCAAGT
GACAGCCACAGATGCAGATGACCCGACCTACGGCAACAGTGCCAGGGTGGTGTACAGCATTCTTCAGGGC
CAGCCATATTTTTCTGTGGACTCTAAAACAGGTGTAATTAGGACAGCGCTCATGAACATGGACAGAGAAG
CCAAAGAATACTACGAAGTGATTATCCAAGCCAAGGACATGGGAGGGCAGCTTGGAGGATTAGCTGGGAC
CACAACAGTGAACATCACCCTCTCAGATGTCAATGATAACCCACCCCGCTTTCCCCAGAAACATTACCAG
ATGAGTGTGTTGGAATCAGCTCCAATTAGCTCCACTGTCGGGAGAGTGTTTGCCAAGGACTTGGATGAAG
GCATCAATGCAGAGATGAAATATACTATTGTGGATGGAGATGGTGCAGATGCCTTTGACATTAGCACAGA
TCCCAATTTCCAAGTTGGTATCATAACTGTGAAGAAGCCCCTGAGTTTTGAAAGCAAGAAAAGCTACACC
TTAAAGGTGGAGGGAGCCAATCCTCACCTAGAGATGCGTTTTCTGAACTTGGGCCCATTTCAGGACACAA
CAACAGTGCACATCAGTGTGGAAGACGTGGACGAGCCCCCTGTGTTTGAACCTGGCTTTTACTTTGTGGA
GGTGCCTGAGGATGTGGCGATTGGAACAACCATACAGATCATTTCTGCCAAGGACCCAGATGTGACCAAC
AACTCAATCAGATACTCCATTGATAGAAGCAGTGACCCTGGAAGATTTTTCTATGTTGACATTACAACAG
GTGCCCTAATGACAGCAAGACCCCTAGACCGGGAAGAATTTTCTTGGCATAATATCACTGTCCTTGCTAT
GGAAATGAACAATCCCTCCCAGGTTGGAAGTGTTCCTGTCACAATCAAAGTCTTAGATGTGAATGACAAT
GCTCCAGAGTTCCCCAGATTCTATGAAGCTTTTGTCTGTGAGAACGCCAAGGCAGGACAGCTGATCCAGA
CAGTGAGTGCGGTGGACCAAGATGACCCACGCAATGGTCAGCATTTCTACTACAGCTTGGCTCCTGAGGC
TGCTAACAACCCCAACTTTACCATAAGGGACAACCAAGATAACACAGCACGGATTCTAACCAGGAGGTCT
GGTTTCCGGCAGCAGGAGCAGAGTGTCTTTCACCTGCCTATCCTGATAGCAGATAGCGGGCAGCCCGTGC
TGAGCAGCACAGGCACACTGACCATCCAAGTGTGCAGCTGTGATGACGACGGCCACGTCATGTCCTGCAG
CCCAGAGGCCTACATGCTCCCAGTCAGTTTGAGCCGGGGCGCCCTCATTGCCATCCTCGCCTGCATCTTT
GTCCTCTTAGTGCTGGTGTTGCTCATTTTGTCCATGAGGCGGCACCGGAAACAACCATACATCATCGACG
ACGAGGAAAACATCCACGAGAACATCGTCCGCTACGACGACGAGGGCGGCGGCGAGGAGGACACCGAGGC
CTTCGACATCGCGGCCATGTGGAACCCCCGGGAGGCGCAGGCGGGGGCCGCCCCCAAGACGCGGCAGGAC
ATGCTGCCCGAGATCGAGAGCCTCTCCCGCTACGTGCCTCAGACGTGCGCAGTGAACAGCACTGTCCACA
GCTACGTGCTGGCCAAGCTCTACGAGGCCGACATGGACCTGTGGGCACCGCCCTTCGACTCCCTCCAGAC
GTATATGTTCGAGGGGGACGGCTCTGTGGCGGGGTCGCTGAGCTCCCTGCAGTCGGCCACGTCGGACTCG
GAACAGAGCTTCGACTTCCTGACGGACTGGGGGCCCCGCTTCCGGAAGCTGGCCGAGCTCTACGGGGCGT
CGGAGGGACCCGCGCCGCTGTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214769 representing NM_031891
Red=Cloning site Green=Tags(s)

MWTSGRMSNAKNWLGLGMSLYFWGLMDLTTTVLSDTPTPQGELEALLSDKPQSHQRTKRSWVWNQFFVLE
EYTGTDPLYVGKLHSDMDRGDGSIKYILSGEGAGIVFTIDDTTGDIHAIQRLDREERAQYTLRAQALDRR
TGRPMEPESEFIIKIQDINDNEPKFLDGPYVATVPEMSPVGTSVIQVTATDADDPTYGNSARVVYSILQG
QPYFSVDSKTGVIRTALMNMDREAKEYYEVIIQAKDMGGQLGGLAGTTTVNITLSDVNDNPPRFPQKHYQ
MSVLESAPISSTVGRVFAKDLDEGINAEMKYTIVDGDGADAFDISTDPNFQVGIITVKKPLSFESKKSYT
LKVEGANPHLEMRFLNLGPFQDTTTVHISVEDVDEPPVFEPGFYFVEVPEDVAIGTTIQIISAKDPDVTN
NSIRYSIDRSSDPGRFFYVDITTGALMTARPLDREEFSWHNITVLAMEMNNPSQVGSVPVTIKVLDVNDN
APEFPRFYEAFVCENAKAGQLIQTVSAVDQDDPRNGQHFYYSLAPEAANNPNFTIRDNQDNTARILTRRS
GFRQQEQSVFHLPILIADSGQPVLSSTGTLTIQVCSCDDDGHVMSCSPEAYMLPVSLSRGALIAILACIF
VLLVLVLLILSMRRHRKQPYIIDDEENIHENIVRYDDEGGGEEDTEAFDIAAMWNPREAQAGAAPKTRQD
MLPEIESLSRYVPQTCAVNSTVHSYVLAKLYEADMDLWAPPFDSLQTYMFEGDGSVAGSLSSLQSATSDS
EQSFDFLTDWGPRFRKLAELYGASEGPAPLW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031891
ORF Size 2403 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031891.4
RefSeq Size 2855 bp
RefSeq ORF 2406 bp
Locus ID 28316
UniProt ID Q9HBT6, A8K2C5, Q8N9J3
Cytogenetics 18q21.33
Domains CA, Cadherin_C_term
Protein Families Druggable Genome
MW 85.1 kDa
Summary This gene is a type II classical cadherin from the cadherin superfamily and one of three cadherin 7-like genes located in a cluster on chromosome 18. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Since disturbance of intracellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes. provided by RefSeq, Jul 2008
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"NM_031891" in other vectors (6)
SKU Description Size Price
RC214769L1 Lenti ORF clone of Human cadherin 20, type 2 (CDH20), Myc-DDK-tagged 10 ug
$1,033.00
RC214769L2 Lenti ORF clone of Human cadherin 20, type 2 (CDH20), mGFP tagged 10 ug
$1,033.00
RC214769L3 Lenti ORF clone of Human cadherin 20, type 2 (CDH20), Myc-DDK-tagged 10 ug
$1,033.00
RC214769L4 Lenti ORF clone of Human cadherin 20, type 2 (CDH20), mGFP tagged 10 ug
$1,033.00
RG214769 CDH20 (tGFP-tagged) - Human cadherin 20, type 2 (CDH20) 10 ug
$789.00 $933.00
SC107114 CDH20 (untagged)-Human cadherin 20, type 2 (CDH20) 10 ug
$734.00

Plasmid Map for RC214769

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214769
Circular map for RC214769

file-alt Citations

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