FOXD3 (NM_012183) Human Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | FOXD3 |
| Synonyms | AIS1; Genesis; HFH2; VAMAS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012183 |
| ORF Size | 1434 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012183.3 |
| RefSeq Size | 1437 bp |
| RefSeq ORF | 1437 bp |
| Locus ID | 27022 |
| UniProt ID | Q9UJU5 |
| Cytogenetics | 1p31.3 |
| MW | 47.4 kDa |
| Summary | This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. Mutations in this gene cause autoimmune susceptibility 1. provided by RefSeq, Nov 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012183" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214719L1 | Lenti-ORF clone of FOXD3 (Myc-DDK-tagged)-Human forkhead box D3 (FOXD3) | 10 ug |
$986.00
|
|
| RC214719L2 | Lenti-ORF clone of FOXD3 (mGFP-tagged)-Human forkhead box D3 (FOXD3) | 10 ug |
$986.00
|
|
| RC214719L3 | Lenti-ORF clone of FOXD3 (Myc-DDK-tagged)-Human forkhead box D3 (FOXD3) | 10 ug |
$986.00
|
|
| RC214719L4 | Lenti-ORF clone of FOXD3 (mGFP-tagged)-Human forkhead box D3 (FOXD3) | 10 ug |
$986.00
|
|
| RG214719 | FOXD3 (tGFP-tagged) - Human forkhead box D3 (FOXD3) | 10 ug |
$886.00
|
|
| SC303938 | FOXD3 (untagged)-Human forkhead box D3 (FOXD3) | 10 ug |
$686.00
|
|
Citations
FOXD3 inhibits cell proliferation, migration and invasion in nasopharyngeal carcinoma through regulation of the PI3K/Akt pathway
Xie, Xiong, Chen et al
Biochem. Cell Biol. (2020)
Xie, Xiong, Chen et al
Biochem. Cell Biol. (2020)
Cited by 16
AI Summary: OriGene’s FOXD3 plasmid was used to overexpress FOXD3 in nasopharyngeal carcinoma cells, enabling the study to demonstrate FOXD3’s inhibitory role on cell proliferation, migration, and invasion via regulation of the PI3K/Akt pathway.
Snippet: CO2 at 37o C. Cell transfection Lipofectamine 2000 (Invitrogen) was applied to transfect 1 μg FOXD3 plasmid (Origene, Rockville, MD, USA) following the manufacturer's instructions, with empty vector as negative control. Cells were cultured to approximately 80
FOXD3, frequently methylated in colorectal cancer, acts as a tumor suppressor and induces tumor cell apoptosis under ER stress via p53
Xu, Zhu, Liu et al
Carcinogenesis (2019)
Xu, Zhu, Liu et al
Carcinogenesis (2019)
AI Summary: OriGene products, specifically the pCMV6 Entry FOXD3-expressing plasmid and MegaTran 1.0 transfection reagent, were used to restore FOXD3 expression in colorectal cancer cell lines, enabling investigation of its tumor-suppressive functions in this study.
Snippet: CRC cell lines DLD-1 and HT29 were transfected with control or FOXD3-expressing plasmid (pCMV6 Entry;Origene, Rockville, MD) using the MegaTran 1.0 transfection reagent (Origene). Stable FOXD3-expressing clones were selected for further study
Showing 1-2 of 2 papers.
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