NKD2 (NM_033120) Human Tagged ORF Clone

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SKU
RC214631
NKD2 (Myc-DDK-tagged)-Human naked cuticle homolog 2 (Drosophila) (NKD2)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NKD2
Synonyms Naked2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214631 representing NM_033120
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAACTGCAGTCGAAGCACGCCGCCGCCGCCCGCAAGCGGAGAGAGAGCCCGGAAGGGGACAGCT
TCGTGGCGTCCGCGTACGCGAGCGGCCGCAAAGGCGCGGAGGAAGCGGAGCGGCGCGCGCGGGACAAGCA
GGAGCTGCCCAATGGGGACCCCAAGGAGGGGCCTTTCCGGGAGGACCAGTGTCCCCTACAGGTGGCACTC
CCCGCTGAGAAAGCTGAGGGCCGCGAGCACCCGGGACAACTCCTCAGCGCAGATGACGGAGAGAGGGCAG
CAAACCGCGAGGGCCCGCGAGGACCGGGCGGGCAGCGCCTCAACATTGACGCACTCCAGTGCGATGTCTC
GGTGGAGGAGGACGACCGCCAGGAGTGGACGTTCACGCTCTATGACTTTGACAACTGCGGGAAGGTCACC
AGGGAGGACATGTCCAGCCTCATGCACACCATCTATGAGGTCGTGGATGCCTCGGTCAACCACTCCTCGG
GCAGCAGCAAGACCCTCCGTGTGAAGCTAACCGTCAGCCCTGAGCCCTCCAGCAAGAGGAAGGAGGGTCC
TCCTGCTGGCCAGGACCGGGAGCCCACCCGTTGCAGGATGGAGGGTGAACTGGCAGAGGAGCCAAGGGTG
GCTGACAGGAGGTTGTCTGCACACGTCAGGAGGCCCAGTACTGACCCCCAGCCCTGCTCGGAGCGGGGGC
CCTACTGCGTGGACGAGAACACGGAGCGCAGAAACCACTACCTGGACCTCGCCGGGATTGAGAACTACAC
GTCCAGATTCGGCCCTGGGTCCCCTCCTGTGCAAGCAAAGCAGGAGCCCCAGGGCAGGGCCTCGCACCTC
CAGGCCCGGTCCCGCTCCCAGGAGCCAGATACACATGCCGTACACCACCGCAGGTCACAGGTGCTGGTGG
AACACGTCGTGCCAGCCTCGGAGCCTGCTGCCCGGGCCCTGGACACGCAGCCCCGGCCGAAGGGGCCGGA
GAAGCAGTTCCTCAAGTCCCCCAAGGGCTCCGGGAAGCCGCCTGGGGTGCCAGCCAGCAGCAAGTCCGGG
AAAGCCTTCAGCTACTACCTGCCGGCCGTCCTGCCGCCCCAGGCCCCTCAGGACGGCCACCACCTCCCGC
AGCCCCCACCGCCACCCTACGGCCACAAGCGGTACCGCCAAAAGGGCAGGGAGGGCCACTCGCCACTCAA
GGCCCCACACGCTCAGCCTGCCACAGTGGAGCACGAGGTGGTGCGGGACCTGCCGCCCACGCCAGCAGGA
GAGGGCTACGCGGTGCCAGTGATCCAGCGGCACGAGCACCACCACCACCACGAGCACCACCACCACCACC
ACCACCACCACTTCCACCCGTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214631 representing NM_033120
Red=Cloning site Green=Tags(s)

MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVAL
PAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVT
REDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRV
ADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSPPVQAKQEPQGRASHL
QARSRSQEPDTHAVHHRRSQVLVEHVVPASEPAARALDTQPRPKGPEKQFLKSPKGSGKPPGVPASSKSG
KAFSYYLPAVLPPQAPQDGHHLPQPPPPPYGHKRYRQKGREGHSPLKAPHAQPATVEHEVVRDLPPTPAG
EGYAVPVIQRHEHHHHHEHHHHHHHHHFHPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_033120
ORF Size 1353 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033120.4
RefSeq Size 1798 bp
RefSeq ORF 1356 bp
Locus ID 85409
UniProt ID Q969F2
Cytogenetics 5p15.33
Protein Families Druggable Genome
Protein Pathways Wnt signaling pathway
MW 49.9 kDa
Summary This gene encodes a member of a family of proteins that function as negative regulators of Wnt receptor signaling through interaction with Dishevelled family members. The encoded protein participates in the delivery of transforming growth factor alpha-containing vesicles to the cell membrane. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Aug 2012
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Documents
Resources
"NM_033120" in other vectors (6)
SKU Description Size Price
RC214631L1 Lenti ORF clone of Human naked cuticle homolog 2 (Drosophila) (NKD2), Myc-DDK-tagged 10 ug
$757.00
RC214631L2 Lenti ORF clone of Human naked cuticle homolog 2 (Drosophila) (NKD2), mGFP tagged 10 ug
$757.00
RC214631L3 Lenti ORF clone of Human naked cuticle homolog 2 (Drosophila) (NKD2), Myc-DDK-tagged 10 ug
$757.00
RC214631L4 Lenti ORF clone of Human naked cuticle homolog 2 (Drosophila) (NKD2), mGFP tagged 10 ug
$757.00
RG214631 NKD2 (tGFP-tagged) - Human naked cuticle homolog 2 (Drosophila) (NKD2) 10 ug
$657.00
SC313535 NKD2 (untagged)-Human naked cuticle homolog 2 (Drosophila) (NKD2) 10 ug
$457.00

Plasmid Map for RC214631

Download sequence for

Cloning scheme for RC214631

Circular map for RC214631

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