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RNF5 (NM_006913) Human Tagged ORF Clone

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SKU
RC214615
RNF5 (Myc-DDK-tagged)-Human ring finger protein 5 (RNF5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "RNF5 or NM_006913"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF5
Synonyms RING5; RMA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214615 representing NM_006913
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCAGCGGAGGAGGAGGACGGGGGCCCCGAAGGGCCAAATCGCGAGCGGGGCGGGGCGGGCGCGA
CCTTCGAATGTAATATATGTTTGGAGACTGCTCGGGAAGCTGTGGTCAGTGTGTGTGGCCACCTGTACTG
TTGGCCATGTCTTCATCAGTGGCTGGAGACACGGCCAGAACGGCAAGAGTGTCCAGTATGTAAAGCTGGG
ATCAGCAGAGAGAAGGTTGTCCCGCTTTATGGGCGAGGGAGCCAGAAGCCCCAGGATCCCAGATTAAAAA
CTCCACCCCGCCCCCAGGGCCAGAGACCAGCTCCGGAGAGCAGAGGGGGATTCCAGCCATTTGGTGATAC
CGGGGGCTTCCACTTCTCATTTGGTGTTGGTGCTTTTCCCTTTGGCTTTTTCACCACCGTCTTCAATGCC
CATGAGCCTTTCCGCCGGGGTACAGGTGTGGATCTGGGACAGGGTCACCCAGCCTCCAGCTGGCAGGATT
CCCTCTTCCTGTTTCTCGCCATCTTCTTCTTTTTTTGGCTGCTCAGTATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214615 representing NM_006913
Red=Cloning site Green=Tags(s)

MAAAEEEDGGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAG
ISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDTGGFHFSFGVGAFPFGFFTTVFNA
HEPFRRGTGVDLGQGHPASSWQDSLFLFLAIFFFFWLLSI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006913
ORF Size 540 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006913.4
RefSeq Size 1179 bp
RefSeq ORF 543 bp
Locus ID 6048
UniProt ID Q99942, A0A024RCQ4
Cytogenetics 6p21.32
Domains RING
Protein Families Transmembrane
MW 19.7 kDa
Summary The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. provided by RefSeq, Jul 2008
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"NM_006913" in other vectors (6)
SKU Description Size Price
RC214615L1 Lenti ORF clone of Human ring finger protein 5 (RNF5), Myc-DDK-tagged 10 ug
$600.00
RC214615L2 Lenti ORF clone of Human ring finger protein 5 (RNF5), mGFP tagged 10 ug
$600.00
RC214615L3 Lenti ORF clone of Human ring finger protein 5 (RNF5), Myc-DDK-tagged 10 ug
$600.00
RC214615L4 Lenti ORF clone of Human ring finger protein 5 (RNF5), mGFP tagged 10 ug
$600.00
RG214615 RNF5 (tGFP-tagged) - Human ring finger protein 5 (RNF5) 10 ug
$489.00 $500.00
SC115779 RNF5 (untagged)-Human ring finger protein 5 (RNF5) 10 ug
$300.00

Plasmid Map for RC214615

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214615
Circular map for RC214615

file-alt Citations

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