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PRSS45 (NM_199183) Human Tagged ORF Clone

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SKU
RC214567
PRSS45 (Myc-DDK-tagged)-Human protease, serine, 45 (PRSS45)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PRSS45
Synonyms TESSP5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214567 representing NM_199183
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCGCCATTGGCCCTGGGAGGTGAGCCTCCGGATGGAAAATGAGCACGTGTGTGGAGGGGCCCTCA
TTGACCCCAGCTGGGTGGTGACTGCGGCCCACTGCATCCAAGGCACCAAAGAGTACTCAGTGGTGCTTGG
CACCTCCAAGCTGCAGCCCATGAACTTCAGCAGGGCCCTCTGGGTCCCTGTGAGGGACATCATTATGCAC
CCCAAGTACTGGGGCCGGGCCTTCATCATGGGTGACGTTGCCCTTGTCCACCTTCAAACACCTGTCACCT
TCAGCTCCACAGCCAACTCCATGCTGACCCCAGAGCTGCAGGAGGCTGAGGTGTTTATCATGGACAACAA
GAGGTGTGACCGGCATTACAAGAAGTCCTTCTTCCCCCCAGTTGTCCCCCTTGTCCTGGGGGACATGATC
TGTGCCACCAATTATGGGGAAAACTTGTGCTATGGGGATTCTGGAGGGCCATTGGCTTGTGAAGTTGAGG
GCAGATGGATTCTGGCTGGGGTGTTGTCCTGGGAAAAGGCCTGCGTGAAGGCACAGAATCCAGGTGTGTA
CACCCGCATCACCAAATACACCAAATGGATCAAGAAGCAAATGAGCAATGGAGCCTTCTCAGGTCCCTGT
GCCTCTGCCTGCCTCCTGTTCCTGTGCTGGCTGCTGCAGCCCCAGATGGGCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214567 protein sequence
Red=Cloning site Green=Tags(s)

MTRHWPWEVSLRMENEHVCGGALIDPSWVVTAAHCIQGTKEYSVVLGTSKLQPMNFSRALWVPVRDIIMH
PKYWGRAFIMGDVALVHLQTPVTFSSTANSMLTPELQEAEVFIMDNKRCDRHYKKSFFPPVVPLVLGDMI
CATNYGENLCYGDSGGPLACEVEGRWILAGVLSWEKACVKAQNPGVYTRITKYTKWIKKQMSNGAFSGPC
ASACLLFLCWLLQPQMGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_199183
ORF Size 684 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199183.2, NP_954652.2
RefSeq Size 962 bp
RefSeq ORF 687 bp
Locus ID 377047
Cytogenetics 3p21.31
MW 29.3 kDa
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"NM_199183" in other vectors (4)
SKU Description Size Price
RC214567L3 Lenti ORF clone of Human protease, serine, 45 (PRSS45), Myc-DDK-tagged 10 ug
$600.00
RC214567L4 Lenti ORF clone of Human protease, serine, 45 (PRSS45), mGFP tagged 10 ug
$600.00
RG214567 PRSS45 (tGFP-tagged) - Human protease, serine, 45 (PRSS45) 10 ug
$500.00
SC307890 PRSS45 (untagged)-Human protease, serine, 45 (PRSS45) 10 ug
$330.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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