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PIGG (NM_017733) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC214539
PIGG (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class G (PIGG), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $982.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PIGG
Synonyms GPI7; LAS21; MRT53; PRO4405; RLGS1930
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214539 representing NM_017733
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCTGGGCTCCGGGACTTTCGCTACCTGTTGCGTAGCGATCGAGGTGCTAGGGATCGCGGTCTTCC
TTCGGGGATTCTTCCCGGCTCCCGTTCGTTCCTCTGCCAGAGCGGAACACGGAGCGGAGCCCCCAGCGCC
CGAACCCTCGGCTGGAGCCAGTTCTAACTGGACCACGCTGCCACCACCTCTCTTCAGTAAAGTTGTTATT
GTTCTGATAGATGCCTTGAGAGATGATTTTGTGTTTGGGTCAAAGGGTGTGAAATTTATGCCCTACACAA
CTTACCTTGTGGAAAAAGGAGCATCTCACAGTTTTGTGGCTGAAGCAAAGCCACCTACAGTTACTATGCC
TCGAATCAAGGCATTGATGACGGGGAGCCTTCCTGGCTTTGTCGACGTCATCAGGAACCTCAATTCTCCT
GCACTGCTGGAAGACAGTGTGATAAGACAAGCAAAAGCAGCTGGAAAAAGAATAGTCTTTTATGGAGATG
AAACCTGGGTTAAATTATTCCCAAAGCATTTTGTGGAATATGATGGAACAACCTCATTTTTCGTGTCAGA
TTACACAGAGGTGGATAATAATGTCACGAGGCATTTGGATAAAGTATTAAAAAGAGGAGATTGGGACATA
TTAATCCTCCACTACCTGGGGCTGGACCACATTGGCCACATTTCAGGGCCCAACAGCCCCCTGATTGGGC
AGAAGCTGAGCGAGATGGACAGCGTGCTGATGAAGATCCACACCTCACTGCAGTCGAAGGAGAGAGAGAC
GCCTTTACCCAATTTGCTGGTTCTTTGTGGTGACCATGGCATGTCTGAAACAGGAAGTCACGGGGCCTCC
TCCACCGAGGAGGTGAATACACCTCTGATTTTAATCAGTTCTGCGTTTGAAAGGAAACCCGGTGATATCC
GACATCCAAAGCACGTCCAACAGACGGATGTGGCTGCGACACTGGCGATAGCACTTGGCTTACCGATTCC
AAAAGACAGTGTAGGGAGCCTCCTATTCCCAGTTGTGGAAGGAAGACCAATGAGAGAGCAGTTGAGATTT
TTACATTTGAATACAGTGCAGCTTAGTAAACTGTTGCAAGAGAATGTGCCGTCATATGAAAAAGATCCTG
GGTTTGAGCAGTTTAAAATGTCAGAAAGATTGCATGGGAACTGGATCAGACTGTACTTGGAGGAAAAGCA
TTCAGAAGTCCTATTCAACCTGGGCTCCAAGGTTCTCAGGCAGTACCTGGATGCTCTGAAGACGCTGAGC
TTGTCCCTGAGTGCACAAGTGGCCCAGTACGACATCTATTCGATGATGGTTCTCACCCTGCTCCTGCTCA
GCGTCCCACAGGCACTGCGCAGAAAGGCTGAGCTGGAAGTCCCACTGTCATCTCCTGGGTTTTCTCTGCT
CTTTTATTTGGTGATCCTGGTTCTTTCGGCCGTTCACGTCATTGTGTGCACCTCAGCTGAAAGTTCGTGC
TACTTCTGTGGCCTCTCGTGGCTGGCGGCAGGTGGGGTGATGGTGCTGGCCTCGGCGCTGCTGTGTGTGA
TTGTGTCTGTTCTGACCAACGTGCTCGTGGGTGGAAACACCCCAAGGAAGAACCCCATGCATCCCAGCTC
AAGGTGGTCAGAGCTAGACCTTCTTATTCTGTTGGGGACGGCGGGCCACGTCTTGAGCCTGGGCGCCAGC
AGCTTCGTGGAGGAGGAGCACCAGACCTGGTACTTCCTTGTGAACACCCTGTGTCTAGCTCTGAGCCAAG
AAACCTACAGAAACTACTTTCTGGGAGATGACGGTGAGCCTCCGTGTGGCCTCTGTGTGGAACAAGGGCA
TGACGGGGCCACAGCAGCGTGGCAGGACGGGCCTGGCTGTGATGTCCTGGAGCGAGACAAAGGCCACGGA
AGCCCCTCTACCTCCGAAGTGCTCAGAGGCCGCGAGAAGTGGATGGTGCTGGCCAGTCCGTGGCTAATAC
TGGCCTGCTGCCGGCTGCTGCGCTCCCTAAACCAGACAGGTGTGCAGTGGGCTCACCGGCCTGACCTCGG
CCACTGGCTCACCAGCTCTGACCACAAAGCCGAGCTCTCTGTCCTGGCTGCCCTCTCCCTCCTCGTAGTT
TTTGTGCTGGTGCAGAGGGGGTGCTCCCCTGTGTCCAAGGCTGCCCTGGCGCTGGGGCTGCTGGGCGTCT
ACTGCTACCGGGCGGCCATCGGGAGTGTCCGGTTCCCGTGGCGGCCGGACAGCAAGGACATTTCCAAGGG
TATTATTGAAGCTCGTTTTGTTTATGTCTTTGTCCTTGGCATTCTGTTCACGGGCACCAAAGACTTACTT
AAATCTCAAGTCATTGCTGCAGACTTCAAACTCAAGACTGTAGGTTTATGGGAGATATATAGTGGATTAG
TTCTTCTGGCAGCCTTGCTCTTTAGACCACATAATCTTCCGGTCTTAGCATTTAGCCTCTTGATTCAGAC
TCTAATGACTAAATTCATCTGGAAGCCCCTGAGACACGATGCAGCTGAGATTACTGTGATGCATTATTGG
TTTGGTCAAGCATTCTTCTATTTTCAGGGCAACTCCAACAACATTGCCACCGTGGACATCTCCGCAGGCT
TCGTGGGCTTAGACACCTACGTGGAAATCCCAGCCGTGCTCCTGACAGCGTTTGGGACGTACGCAGGGCC
TGTGCTGTGGGCCAGCCACTTAGTGCACTTCCTGAGCTCAGAAACACGCAGTGGTTCAGCACTGAGTCAT
GCTTGCTTCTGCTACGCACTGATTTGTTCTATTCCAGTTTTCACGTACATCGTTTTGGTGACATCTCTGC
GTTATCATTTATTTATATGGAGTGTATTTTCTCCAAAACTTCTCTACGAGGGAATGCACCTGCTCATTAC
AGCTGCTGTCTGTGTATTCTTCACGGCAATGGATCAAACCAGACTCACACAGTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214539 representing NM_017733
Red=Cloning site Green=Tags(s)

MRLGSGTFATCCVAIEVLGIAVFLRGFFPAPVRSSARAEHGAEPPAPEPSAGASSNWTTLPPPLFSKVVI
VLIDALRDDFVFGSKGVKFMPYTTYLVEKGASHSFVAEAKPPTVTMPRIKALMTGSLPGFVDVIRNLNSP
ALLEDSVIRQAKAAGKRIVFYGDETWVKLFPKHFVEYDGTTSFFVSDYTEVDNNVTRHLDKVLKRGDWDI
LILHYLGLDHIGHISGPNSPLIGQKLSEMDSVLMKIHTSLQSKERETPLPNLLVLCGDHGMSETGSHGAS
STEEVNTPLILISSAFERKPGDIRHPKHVQQTDVAATLAIALGLPIPKDSVGSLLFPVVEGRPMREQLRF
LHLNTVQLSKLLQENVPSYEKDPGFEQFKMSERLHGNWIRLYLEEKHSEVLFNLGSKVLRQYLDALKTLS
LSLSAQVAQYDIYSMMVLTLLLLSVPQALRRKAELEVPLSSPGFSLLFYLVILVLSAVHVIVCTSAESSC
YFCGLSWLAAGGVMVLASALLCVIVSVLTNVLVGGNTPRKNPMHPSSRWSELDLLILLGTAGHVLSLGAS
SFVEEEHQTWYFLVNTLCLALSQETYRNYFLGDDGEPPCGLCVEQGHDGATAAWQDGPGCDVLERDKGHG
SPSTSEVLRGREKWMVLASPWLILACCRLLRSLNQTGVQWAHRPDLGHWLTSSDHKAELSVLAALSLLVV
FVLVQRGCSPVSKAALALGLLGVYCYRAAIGSVRFPWRPDSKDISKGIIEARFVYVFVLGILFTGTKDLL
KSQVIAADFKLKTVGLWEIYSGLVLLAALLFRPHNLPVLAFSLLIQTLMTKFIWKPLRHDAAEITVMHYW
FGQAFFYFQGNSNNIATVDISAGFVGLDTYVEIPAVLLTAFGTYAGPVLWASHLVHFLSSETRSGSALSH
ACFCYALICSIPVFTYIVLVTSLRYHLFIWSVFSPKLLYEGMHLLITAAVCVFFTAMDQTRLTQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017733
ORF Size 2925 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017733.4
RefSeq Size 3242 bp
RefSeq ORF 2928 bp
Locus ID 54872
UniProt ID Q5H8A4
Cytogenetics 4p16.3
Protein Families Transmembrane
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
MW 107.4 kDa
Summary This gene encodes an enzyme involved in glycosylphosphatidylinositol-anchor biosynthesis. The encoded protein, which is localized to the endoplasmic reticulum, is involved in transferring ethanoloamine phosphate to mannose 2 of glycosylphosphatidylinositol species H7 to form species H8. Allelic variants of this gene have been associated with intellectual disability, hypotonia, and early-onset seizures. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2016
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Documents
Resources
"NM_017733" in other vectors (5)
SKU Description Size Price
RC214539L3 Lenti-ORF clone of PIGG (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class G (PIGG), transcript variant 2 10 ug
$1,282.00
RC214539L4 Lenti-ORF clone of PIGG (mGFP-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class G (PIGG), transcript variant 2 10 ug
$1,282.00
RG214539 PIGG (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class G (PIGG), transcript variant 2 10 ug
$1,182.00
SC114010 PIGG (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class G (PIGG), transcript variant 2 10 ug
$563.00
SC318423 PIGG (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class G (PIGG), transcript variant 2 10 ug
$983.00

Plasmid Map for RC214539

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214539
Circular map for RC214539

file-alt Citations

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