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CDC25C (NM_022809) Human Tagged ORF Clone

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SKU
RC214496
CDC25C (Myc-DDK-tagged)-Human cell division cycle 25 homolog C (S. pombe) (CDC25C), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDC25C
Synonyms CDC25; PPP1R60
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214496 representing NM_022809
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTACGGAACTCTTCTCATCCACAAGAGAGGAAGGAAGCTCTGGCTCAGGACCCAGTTTTAGGTCTA
ATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGACACTTCCTTTACCGTCTGTCCAGATGTCCCTAG
AACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATTTTGTCTGGGTCACCTGGATTCTTC
AGGACTTCAGGAAGTGCATTTAGCTGGGATGACAATGGAAACTTGGTGGACAGTGAAATGAAATATTTGG
GCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAATCCAAACCTAGGAGAAGACCAGGCAGAAGAGAT
TTCAGATGAATTAATGGAGTTTTCCCTGAAAGATCAAGAAGCAAAGGTGAGCAGAAGTGGCCTATATCGC
TCCCCGTCGATGCCAGAGAACTTGAACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAAGGACAACACAA
TACCAGATAAAGTTAAAAAAAAGTATTTTTCTGGCCAAGGAAAGCTCAGGAAGGGCTTATGTTTAAAGAA
GACAGTCTCTCTGTGTGACATTACTATCACTCAGATGCTGGAGGAAGATTCTAACCAGGGGCACCTGATT
GGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGTCAGGGAAACACCAAGATCTGAAGTATGTCAACC
CAGAAACAGTGGCTGCCTTACTGTCGGGGAAGTTCCAGGGTCTGATTGAGAAGTTTTATGTCATTGATTG
TCGCTATCCATATGAGTATCTGGGAGGACACATCCAGGGAGCCTTAAACTTATATAGTCAGGAAGAACTG
TTTAACTTCTTTCTGAAGAAGCCCATCGTCCCTTTGGACACCCAGAAGAGAATAATCATCGTGTTCCACT
GTGAATTCTCCTCAGAGAGGGGCCCCCGAATGTGCCGCTGTCTGCGTGAAGAGGACAGGTCTCTGAACCA
GTATCCTGCATTGTACTACCCAGAGCTATATATCCTTAAAGGCGGCTACAGAGACTTCTTTCCAGAATAT
ATGGAACTGTGTGAACCACAGAGCTACTGCCCTATGCATCATCAGGACCACAAGACTGAGTTGCTGAGGT
GTCGAAGCCAGAGCAAAGTGCAGGAAGGGGAGCGGCAGCTGCGGGAGCAGATTGCCCTTCTGGTGAAGGA
CATGAGCCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214496 representing NM_022809
Red=Cloning site Green=Tags(s)

MSTELFSSTREEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGSPGFF
RTSGSAFSWDDNGNLVDSEMKYLGSPITTVPKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSGLYR
SPSMPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCDITITQMLEEDSNQGHLI
GDFSKVCALPTVSGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEEL
FNFFLKKPIVPLDTQKRIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILKGGYRDFFPEY
MELCEPQSYCPMHHQDHKTELLRCRSQSKVQEGERQLREQIALLVKDMSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022809
ORF Size 1200 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022809.3, NP_073720.1
RefSeq Size 1896 bp
RefSeq ORF 1203 bp
Locus ID 995
UniProt ID P30307
Cytogenetics 5q31.2
Protein Families Druggable Genome, Phosphatase, Stem cell - Pluripotency
Protein Pathways Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
MW 45.4 kDa
Summary This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. provided by RefSeq, Dec 2015
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Documents
Resources
"NM_022809" in other vectors (4)
SKU Description Size Price
RC214496L3 Lenti ORF clone of Human cell division cycle 25 homolog C (S. pombe) (CDC25C), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC214496L4 Lenti ORF clone of Human cell division cycle 25 homolog C (S. pombe) (CDC25C), transcript variant 2, mGFP tagged 10 ug
$757.00
RG214496 CDC25C (tGFP-tagged) - Human cell division cycle 25 homolog C (S. pombe) (CDC25C), transcript variant 2 10 ug
$657.00
SC305053 CDC25C (untagged)-Human cell division cycle 25 homolog C (S. pombe) (CDC25C), transcript variant 2 10 ug
$503.00

Plasmid Map for RC214496

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214496
Circular map for RC214496

file-alt Citations

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