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H2BC7 (NM_003522) Human Tagged ORF Clone

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SKU
RC214442
HIST1H2BF (Myc-DDK-tagged)-Human histone cluster 1, H2bf (HIST1H2BF)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol H2BC7
Synonyms H2B/g; H2BC4; H2BC6; H2BC8; H2BC10; H2BFG; HIST1H2BF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214442 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGAACCTGCTAAGTCCGCTCCTGCTCCAAAAAAGGGCTCCAAAAAGGCGGTGACCAAGGCGCAGA
AGAAGGATGGTAAGAAGCGCAAGCGTAGCCGCAAGGAGAGCTATTCCGTGTACGTGTACAAGGTGCTAAA
GCAGGTCCACCCCGACACCGGCATCTCATCCAAGGCCATGGGCATCATGAACTCCTTCGTCAACGATATC
TTCGAGCGCATCGCTGGCGAGGCTTCCCGCCTGGCGCATTACAACAAGCGCTCCACCATCACCTCCAGGG
AGATCCAGACGGCCGTACGCCTGCTGCTGCCCGGGGAGCTGGCTAAGCACGCCGTGTCAGAGGGCACCAA
GGCCGTCACCAAGTACACCAGCTCTAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214442 protein sequence
Red=Cloning site Green=Tags(s)

MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDI
FERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003522
ORF Size 378 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003522.4
RefSeq Size 430 bp
RefSeq ORF 381 bp
Locus ID 8343
UniProt ID P62807, B2R4S9
Cytogenetics 6p22.2
Domains H2B, histone
Protein Pathways Systemic lupus erythematosus
MW 13.9 kDa
Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. The protein has antibacterial and antifungal antimicrobial activity. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. provided by RefSeq, Aug 2015
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"NM_003522" in other vectors (4)
SKU Description Size Price
RC214442L3 Lenti ORF clone of Human histone cluster 1, H2bf (HIST1H2BF), Myc-DDK-tagged 10 ug
$450.00
RC214442L4 Lenti ORF clone of Human histone cluster 1, H2bf (HIST1H2BF), mGFP tagged 10 ug
$450.00
RG214442 HIST1H2BF (tGFP-tagged) - Human histone cluster 1, H2bf (HIST1H2BF) 10 ug
$350.00
SC309006 HIST1H2BF (untagged)-Human histone cluster 1, H2bf (HIST1H2BF) 10 ug
$165.00

Plasmid Map for RC214442

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214442
Circular map for RC214442

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