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PPIL2 (NM_148176) Human Tagged ORF Clone

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SKU
RC214404
PPIL2 (Myc-DDK-tagged)-Human peptidylprolyl isomerase (cyclophilin)-like 2 (PPIL2), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $491.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPIL2
Synonyms CYC4; Cyp-60; CYP60; hCyP-60; UBOX7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214404 representing NM_148176
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCACTTTTATGGTG
GCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGACCACTGCAGTCTCTCTCTGCA
GCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTCTTTGACTTACTGAACATTGTTCCATGGCTT
AAGAAGTACGGGACCAACCCCAGCAATGGAGAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTT
CCAAGAACAGTGAGGGGAAGTACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGT
GGCTGTGAGGACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGACCCCACCAATT
TGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAAATAATAGACCCAGATGAAGA
GAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACAAATGCCGAGACCCGAGAGACCCTGCAGGAG
CTCTACAAGGAGTTCAAAGGGGACGAGATTCTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGG
ACAAGCTGAACGCTGCCCACTATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCC
GGAGACCACACATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACACCAAAAACCT
GCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATCTTCCACAGATCCATCCGGAA
CTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACGGGTGGGGAGTCATACTGGGGGAAGCCCTTC
AAAGACGAGTTCCGGCCCAACCTCTCGCACACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCA
ACAGCAACAGGTCTCAATTCTTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTT
TGGACGGGTTGTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAGGCCGATGCCC
AGATTGCGCAGGAGCGGAAGACACAGCTCAAGGTAGCCCCGGAGACCAAAGTGAAGAGCAGCCAGCCCCA
GGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTGGGCAAGTACATCAACCCAGCAGCCACCGAG
CAGCAGAGGAAGAGCCCTCAACCAGTGCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGG
GGACTTCAGCTCCTGGTAGCAGCAGGCTGCCTGATGACCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214404 representing NM_148176
Red=Cloning site Green=Tags(s)

MGKRQHQKDKMYITCAEYTHFYGGKKPDLPQTNFRRLPFDHCSLSLQPFVYPVCTPDGIVFDLLNIVPWL
KKYGTNPSNGEKLDGRSLIKLNFSKNSEGKYHCPVLFTVFTNNTHIVAVRTTGNVYAYEAVEQLNIKAKN
FRDLLTDEPFSRQDIITLQDPTNLDKFNVSNFYHVKNNMKIIDPDEEKAKQDPSYYLKNTNAETRETLQE
LYKEFKGDEILAATMKAPEKKKVDKLNAAHYSTGKVSASFTSTAMVPETTHEAAAIDEDVLRYQFVKKKG
YVRLHTNKGDLNLELHCDLTPKTCENFIRLCKKHYYDGTIFHRSIRNFVIQGGDPTGTGTGGESYWGKPF
KDEFRPNLSHTGRGILSMANSGPNSNRSQFFITFRSCAYLDKKHTIFGRVVGGFDVLTAMENVESDPKTD
RPKEEIRIDATTVFVDPYEEADAQIAQERKTQLKVAPETKVKSSQPQAGSQGPQTFRQGVGKYINPAATE
QQRKSPQPVPLSPCPRRSPVGVLGTSAPGSSRLPDDH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_148176
ORF Size 1581 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_148176.2, NP_680481.1
RefSeq Size 2736 bp
RefSeq ORF 1584 bp
Locus ID 23759
UniProt ID Q13356
Cytogenetics 22q11.21
Domains pro_isomerase
Protein Families Druggable Genome
Protein Pathways Ubiquitin mediated proteolysis
MW 59.5 kDa
Summary This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Dec 2015
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Documents
Resources
"NM_148176" in other vectors (4)
SKU Description Size Price
RC214404L3 Lenti-ORF clone of PPIL2 (Myc-DDK-tagged)-Human peptidylprolyl isomerase (cyclophilin)-like 2 (PPIL2), transcript variant 3 10 ug
$791.00
RC214404L4 Lenti-ORF clone of PPIL2 (mGFP-tagged)-Human peptidylprolyl isomerase (cyclophilin)-like 2 (PPIL2), transcript variant 3 10 ug
$791.00
RG214404 PPIL2 (tGFP-tagged) - Human peptidylprolyl isomerase (cyclophilin)-like 2 (PPIL2), transcript variant 3 10 ug
$691.00
SC101193 PPIL2 (untagged)-Human peptidylprolyl isomerase (cyclophilin)-like 2 (PPIL2), transcript variant 3 10 ug
$835.00

Plasmid Map for RC214404

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214404
Circular map for RC214404

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