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TTLL7 (NM_024686) Human Tagged ORF Clone

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SKU
RC214379
TTLL7 (Myc-DDK-tagged)-Human tubulin tyrosine ligase-like family, member 7 (TTLL7)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $812.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TTLL7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214379 representing NM_024686
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATCTCTGCCTCAAGAAGGAGTTATTCAGGGACCCTCTCCCCTGGATTTGAATACAGAATTACCTT
ATCAAAGCACAATGAAAAGGAAAGTCAGAAAGAAGAAAAAGAAGGGAACCATTACAGCAAATGTTGCCGG
GACAAAGTTTGAAATTGTTCGTTTAGTAATAGATGAAATGGGATTTATGAAAACTCCAGATGAGGATGAA
ACAAGTAATCTTATATGGTGTGATTCTGCTGTTCAGCAGGAGAAAATTTCAGAGCTGCAAAATTATCAGA
GGATCAACCATTTTCCAGGAATGGGGGAGATCTGTAGGAAGGATTTCTTAGCAAGAAATATGACCAAAAT
GATCAAGTCTCGGCCTCTGGATTATACCTTTGTTCCTCGAACTTGGATCTTTCCTGCTGAATATACTCAA
TTCCAAAATTATGTGAAAGAATTGAAGAAAAAACGGAAGCAGAAAACTTTTATAGTGAAACCAGCTAATG
GTGCAATGGGTCATGGGATTTCTTTGATAAGAAATGGTGACAAACTTCCATCTCAGGATCATTTGATTGT
TCAAGAATACATTGAAAAGCCTTTCCTAATGGAAGGTTACAAGTTTGACTTACGAATTTATATTCTGGTT
ACATCGTGTGATCCACTAAAAATATTTCTCTACCATGATGGGCTTGTGCGAATGGGTACAGAGAAGTACA
TTCCACCTAATGAGTCCAATTTGACCCAGTTATACATGCATCTGACAAACTACTCCGTGAACAAGCATAA
TGAGCATTTTGAACGGGATGAAACTGAGAACAAAGGCAGCAAACGTTCCATCAAATGGTTTACAGAATTC
CTTCAAGCAAATCAACATGATGTTGCTAAGTTTTGGAGTGATATTTCAGAATTGGTGGTAAAGACCCTGA
TTGTAGCAGAACCTCATGTCCTGCATGCCTATCGAATGTGTAGACCTGGTCAACCTCCAGGAAGCGAAAG
TGTCTGCTTTGAAGTCCTGGGATTTGATATTTTGTTGGATAGAAAACTAAAGCCATGGCTTCTGGAGATT
AACCGAGCCCCAAGCTTTGGAACTGATCAGAAAATAGACTATGATGTAAAAAGGGGAGTGCTGCTAAATG
CGTTGAAGCTACTAAACATAAGGACCAGTGACAAAAGAAGAAACTTGGCCAAACAAAAAGCTGAGGCTCA
AAGGAGGCTCTATGGTCAAAATTCAATTAAAAGGCTCTTACCAGGCTCCTCAGACTGGGAACAGCAGAGA
CACCAGTTGGAGAGGCGGAAAGAAGAGTTGAAAGAGAGACTCGCTCAAGTACGAAAGCAGATCTCACGAG
AAGAACATGAAAATCGACATATGGGGAATTATAGACGAATTTATCCTCCTGAAGATAAAGCATTACTTGA
AAAGTATGAAAATTTGTTAGCTGTTGCCTTTCAGACCTTCCTTTCAGGAAGAGCAGCTTCATTCCAGCGA
GAGTTGAATAATCCTTTGAAAAGGATGAAGGAAGAAGATATTTTGGATCTTCTGGAGCAATGTGAAATTG
ATGATGAAAAGTTGATGGGAAAAACTACCAAGACTCGAGGACCAAAGCCTCTGTGTTCTATGCCTGAGAG
TACTGAGATAATGAAAAGACCAAAGTACTGCAGCAGTGACAGCAGTTATGATAGTAGCAGCAGCTCTTCA
GAATCTGACGAAAATGAAAAAGAAGAGTACCAAAATAAGAAAAGAGAAAAGCAAGTTACATATAATCTTA
AACCCTCCAACCACTACAAATTAATTCAACAACCCAGCTCCATAAGACGTTCAGTCAGCTGCCCTCGGTC
CATCTCTGCTCAATCACCTTCCAGTGGGGACACCCGCCCATTTTCTGCTCAACAAATGATATCTGTGTCA
CGGCCAACTTCTGCATCTCGGTCACATTCCTTAAACCGTGCTTCCTCCTACATGAGGCATCTGCCTCACA
GTAATGATGCCTGCTCTACCAACTCTCAAGTGAGTGAGTCTTTGCGGCAACTGAAAACAAAAGAACAAGA
AGATGATCTAACAAGTCAGACCTTATTTGTTCTCAAAGACATGAAGATCCGGTTTCCAGGAAAGTCAGAT
GCAGAATCAGAACTTCTGATAGAAGATATCATTGATAACTGGAAGTATCATAAAACCAAAGTGGCTTCAT
ATTGGCTCATAAAATTGGACTCTGTAAAACAACGAAAAGTTTTGGACATAGTGAAAACAAGTATTCGTAC
AGTTCTTCCACGCATCTGGAAGGTGCCTGATGTTGAAGAAGTAAATTTATATCGGATTTTCAACCGGGTT
TTTAATCGCTTACTCTGGAGTCGTGGCCAAGGGCTGTGGAACTGTTTCTGTGATTCAGGATCCTCTTGGG
AGAGTATATTCAATAAAAGCCCGGAGGTGGTGACTCCTTTGCAGCTCCAGTGTTGCCAGCGCCTAGTGGA
GCTTTGTAAACAGTGCCTGCTAGTGGTTTACAAATATGCAACTGACAAAAGAGGATCACTTTCAGGCATT
GGTCCTGACTGGGGTAATTCCAGGTATTTACTACCAGGGAGCACCCAATTCTTCTTGAGAACACCAACCT
ACAACTTGAAGTACAATTCACCTGGAATGACTCGCTCCAATGTTTTGTTTACATCCAGATATGGCCATCT
G

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214379 representing NM_024686
Red=Cloning site Green=Tags(s)

MPSLPQEGVIQGPSPLDLNTELPYQSTMKRKVRKKKKKGTITANVAGTKFEIVRLVIDEMGFMKTPDEDE
TSNLIWCDSAVQQEKISELQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPLDYTFVPRTWIFPAEYTQ
FQNYVKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKLPSQDHLIVQEYIEKPFLMEGYKFDLRIYILV
TSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNEHFERDETENKGSKRSIKWFTEF
LQANQHDVAKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEI
NRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRRNLAKQKAEAQRRLYGQNSIKRLLPGSSDWEQQR
HQLERRKEELKERLAQVRKQISREEHENRHMGNYRRIYPPEDKALLEKYENLLAVAFQTFLSGRAASFQR
ELNNPLKRMKEEDILDLLEQCEIDDEKLMGKTTKTRGPKPLCSMPESTEIMKRPKYCSSDSSYDSSSSSS
ESDENEKEEYQNKKREKQVTYNLKPSNHYKLIQQPSSIRRSVSCPRSISAQSPSSGDTRPFSAQQMISVS
RPTSASRSHSLNRASSYMRHLPHSNDACSTNSQVSESLRQLKTKEQEDDLTSQTLFVLKDMKIRFPGKSD
AESELLIEDIIDNWKYHKTKVASYWLIKLDSVKQRKVLDIVKTSIRTVLPRIWKVPDVEEVNLYRIFNRV
FNRLLWSRGQGLWNCFCDSGSSWESIFNKSPEVVTPLQLQCCQRLVELCKQCLLVVYKYATDKRGSLSGI
GPDWGNSRYLLPGSTQFFLRTPTYNLKYNSPGMTRSNVLFTSRYGHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024686
ORF Size 2661 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024686.6
RefSeq Size 3648 bp
RefSeq ORF 2664 bp
Locus ID 79739
UniProt ID Q6ZT98
Cytogenetics 1p31.1
MW 102.8 kDa
Summary Polyglutamylase which preferentially modifies beta-tubulin (PubMed:25959773). Mediates both ATP-dependent initiation and elongation of polyglutamylation of microtubules (PubMed:25959773). Required for neurite growth; responsible for the strong increase in tubulin polyglutamylation during postnatal neuronal maturation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024686" in other vectors (4)
SKU Description Size Price
RC214379L3 Lenti ORF clone of Human tubulin tyrosine ligase-like family, member 7 (TTLL7), Myc-DDK-tagged 10 ug
$1,112.00
RC214379L4 Lenti ORF clone of Human tubulin tyrosine ligase-like family, member 7 (TTLL7), mGFP tagged 10 ug
$1,112.00
RG214379 TTLL7 (tGFP-tagged) - Human tubulin tyrosine ligase-like family, member 7 (TTLL7) 10 ug
$1,012.00
SC318361 TTLL7 (untagged)-Human tubulin tyrosine ligase-like family, member 7 (TTLL7) 10 ug
$894.00

Plasmid Map for RC214379

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214379
Circular map for RC214379

file-alt Citations

Structural and Molecular Basis for Katanin-Mediated Severing of Glutamylated Microtubules
Shin, Im, Jang et al
Cell Rep (2019) 26 (5), 1357-1367.e5 DOI: 10.1016/j.celrep.2019.01.020
AI Summary: OriGene products Myc-DDK-TTLL5 (RC211990), Myc-DDK-TTLL7 (RC214379), and Myc-DDK-Human spastin (SPAST, RC214100) were used as plasmids in this study to investigate the structural and functional mechanisms of katanin-mediated microtubule severing.
Snippet: Plasmid: Myc-Kp60_R283E This paper N/A Plasmid: Myc-Kp60_R283K This paper N/A Plasmid: Myc-DDK-TTLL5 Origene RC211990 Plasmid: Myc-DDK-TTLL7 Origene RC214379 Plasmid: V5-TTLL7 This paper N/A Plasmid: Myc-DDK-Human spastin (SPAST) Origene RC214100 Plasmid: pET32a-Kp60AAA (183-491 a.a.) This paper N/A Plasmid: pET32a-Kp60AAA_E309Q This paper N/A
Showing 1-1 of 1 papers.
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