BTNL2 (NM_019602) Human Tagged ORF Clone

  • TrueORF®
SKU
RC214359
BTNL2 (Myc-DDK-tagged)-Human butyrophilin-like 2 (MHC class II associated) (BTNL2)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BTNL2
Synonyms BTL-II; BTN7; HSBLMHC1; SS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214359 representing NM_019602
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGATTTTCCAGGCTACAATCTGTCTGGTGCAGTCGCCTCCTTCCTATTCATCCTGCTGACAATGA
AGCAGTCAGAAGACTTTAGAGTCATTGGCCCTGCTCATCCTATCCTGGCCGGGGTTGGGGAAGATGCCCT
GTTAACCTGCCAGCTACTCCCCAAGAGGACCACAATGCACGTGGAGGTGAGGTGGTACCGCTCAGAGCCC
AGCACACCTGTGTTTGTGCACAGGGATGGAGTGGAGGTGACTGAGATGCAGATGGAGGAGTACAGAGGCT
GGGTAGAGTGGATAGAGAATGGCATTGCAAAGGGAAATGTGGCACTGAAGATACACAACATCCAGCCCTC
CGACAATGGACAATACTGGTGCCATTTCCAGGATGGGAACTACTGTGGAGAAACAAGCTTGCTGCTCAAA
GTAGCAGGTCTGGGGTCTGCCCCTAGCATCCACATGGAGGGACCTGGGGAGAGTGGAGTCCAGCTTGTGT
GCACTGCAAGGGGCTGGTTCCCAGAGCCCCAGGTGTATTGGGAAGACATCCGGGGAGAGAAGCTGCTGGC
CGTGTCTGAGCATCGCATCCAAGATAAAGATGGCCTGTTCTATGCGGAAGCCACCCTGGTGGTCAGGAAC
GCCTCTGCAGAGTCTGTGTCCTGCTTGGTCCACAACCCCGTCCTCACTGAGGAGAAGGGGTCGGTCATCA
GCCTCCCAGAGAAACTCCAGACTGAGCTGGCTTCTTTAAAAGTGAATGGACCTTCCCAGCCCATCCTCGT
CAGAGTGGGAGAAGATATACAGCTAACCTGTTACCTGTCCCCCAAGGCGAATGCACAGAGCATGGAGGTG
AGGTGGGACCGATCCCACCGTTACCCTGCTGTGCATGTGTATATGGATGGGGACCATGTGGCTGGAGAGC
AGATGGCAGAGTACAGAGGGAGGACTGTACTGGTGAGTGACGCCATTGACGAGGGCAGACTGACCCTGCA
GATACTCAGTGCCAGACCTTCGGACGACGGGCAGTACCGCTGCCTTTTTGAAAAAGATGATGTCTACCAG
GAGGCCAGTTTGGATCTGAAGGTGGTAAGTCTGGGTTCTTCCCCACTGATCACTGTGGAGGGGCAAGAAG
ATGGAGAAATGCAGCCGATGTGCTCTTCAGATGGGTGGTTCCCACAGCCCCACGTGCCATGGAGGGACAT
GGAAGGAAAGACGATACCATCATCTTCCCAGGCCCTGACTCAAGGCAGCCACGGGCTGTTCCACGTGCAG
ACATTGCTAAGGGTCACAAACATCTCCGCTGTGGACGTCACTTGTTCCATCAGCATCCCCTTTTTGGGCG
AGGAGAAAATCGCAACTTTTTCTCTCTCAGGTTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214359 representing NM_019602
Red=Cloning site Green=Tags(s)

MVDFPGYNLSGAVASFLFILLTMKQSEDFRVIGPAHPILAGVGEDALLTCQLLPKRTTMHVEVRWYRSEP
STPVFVHRDGVEVTEMQMEEYRGWVEWIENGIAKGNVALKIHNIQPSDNGQYWCHFQDGNYCGETSLLLK
VAGLGSAPSIHMEGPGESGVQLVCTARGWFPEPQVYWEDIRGEKLLAVSEHRIQDKDGLFYAEATLVVRN
ASAESVSCLVHNPVLTEEKGSVISLPEKLQTELASLKVNGPSQPILVRVGEDIQLTCYLSPKANAQSMEV
RWDRSHRYPAVHVYMDGDHVAGEQMAEYRGRTVLVSDAIDEGRLTLQILSARPSDDGQYRCLFEKDDVYQ
EASLDLKVVSLGSSPLITVEGQEDGEMQPMCSSDGWFPQPHVPWRDMEGKTIPSSSQALTQGSHGLFHVQ
TLLRVTNISAVDVTCSISIPFLGEEKIATFSLSGW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019602
ORF Size 1365 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019602.1, NP_062548.1
RefSeq Size 1368 bp
RefSeq ORF 1368 bp
Locus ID 56244
Cytogenetics 6p21.32
Protein Families Druggable Genome, Secreted Protein
MW 50.3 kDa
Summary This gene encodes a major histocompatibility complex, class II associated, type I transmembrane protein which belongs to the butyrophilin-like B7 family of immunoregulators. It is thought to be involved in immune surveillance, serving as a negative T-cell regulator by decreasing T-cell proliferation and cytokine release. The encoded protein contains an N-terminal signal peptide, two pairs of immunoglobulin-like domains, separated by a heptad peptide sequence, and a C-terminal transmembrane domain. Naturally occurring mutations in this gene are associated with sarcoidosis, rheumatoid arthritis, ulcerative colitis, inflammatory bowel disease, myositis, type 1 diabetes, systemic lupus erythematosus, acute coronary syndrome, and prostate cancer. provided by RefSeq, May 2017
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Documents
Resources
"NM_019602" in other vectors (6)
SKU Description Size Price
RC214359L1 Lenti-ORF clone of BTNL2 (Myc-DDK-tagged)-Human butyrophilin-like 2 (MHC class II associated) (BTNL2) 10 ug
$986.00
RC214359L2 Lenti-ORF clone of BTNL2 (mGFP-tagged)-Human butyrophilin-like 2 (MHC class II associated) (BTNL2) 10 ug
$986.00
RC214359L3 Lenti-ORF clone of BTNL2 (Myc-DDK-tagged)-Human butyrophilin-like 2 (MHC class II associated) (BTNL2) 10 ug
$986.00
RC214359L4 Lenti-ORF clone of BTNL2 (mGFP-tagged)-Human butyrophilin-like 2 (MHC class II associated) (BTNL2) 10 ug
$986.00
RG214359 BTNL2 (tGFP-tagged) - Human butyrophilin-like 2 (MHC class II associated) (BTNL2) 10 ug
$886.00
SC304682 BTNL2 (untagged)-Human butyrophilin-like 2 (MHC class II associated) (BTNL2) 10 ug
$457.00

Plasmid Map for RC214359

Download sequence for

Cloning scheme for RC214359

Circular map for RC214359

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