HOMER2 (NM_199331) Human Tagged ORF Clone

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SKU
RC214311
HOMER2 (Myc-DDK-tagged)-Human homer homolog 2 (Drosophila) (HOMER2), transcript variant 3
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HOMER2
Synonyms ACPD; CPD; HOMER-2; HOMER2A; HOMER2B; Vesl-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214311 representing NM_199331
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGAACAGCCCATCTTCACCACCCGAGCGCATGTCTTCCAGATTGACCCCAACACCAAGAAGAACT
GGATGCCTGCGAGCAAGCAGGCGGTCACCGTTTCCTACTTCTATGATGTCACAAGGAACAGCTATCGGAT
CATCAGTGTGGACGGAGCCAAGGTGATCATAAACAGCACAATCACACCGAATATGACCTTCACCAAAACG
TCACAGAAGTTTGGGCAGTGGGCCGACAGCAGAGCCAACACAGTGTTTGGTTTGGGGTTTTCCTCTGAGC
AGCAGCTGACAAAGTTTGCAGAGAAATTCCAGGAGGTGAAAGAAGCTGCCAAGATAGCCAAAGACAAGAC
GCAGGAGAAAATCGAGACCTCAAGTAATCATTCCCAAGCATCCAGTGTCAACGGGACGGACGATGAAAAG
GCCTCTCACGCCGGTCCAGCCAACACACACCTGAAGTCTGAGAATGACAAGCTGAAGATTGCCTTGACGC
AGAGCGCAGCCAACGTGAAGAATGAGATCAACAGAGAGAAGGAGAAGAACACGCAGCTGAAGAGGAGGAT
CGAGGAGCTGGAGGCAGAGCTCCGAGAAAAGGAGACAGAGCTGAAAGATCTCCGAAAACAAAGTGAAATC
ATACCTCAGCTCATGTCAGAGTGCGAATATGTCTCTGAGAAGCTAGAGGCGGCAGAGAGAGACAATCAAA
ACCTGGAAGACAAAGTGCGTTCCTTAAAGACAGACATTGAGGAGAGCAAATACCGACAGCGCCACCTGAA
GGTGGAGTTGAAGAGCTTCCTGGAGGTGCTGGACGGGAAGATTGACGACCTGCATGACTTCCGCCGAGGG
CTCTCCAAGCTGGGCACCGATAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214311 representing NM_199331
Red=Cloning site Green=Tags(s)

MGEQPIFTTRAHVFQIDPNTKKNWMPASKQAVTVSYFYDVTRNSYRIISVDGAKVIINSTITPNMTFTKT
SQKFGQWADSRANTVFGLGFSSEQQLTKFAEKFQEVKEAAKIAKDKTQEKIETSSNHSQASSVNGTDDEK
ASHAGPANTHLKSENDKLKIALTQSAANVKNEINREKEKNTQLKRRIEELEAELREKETELKDLRKQSEI
IPQLMSECEYVSEKLEAAERDNQNLEDKVRSLKTDIEESKYRQRHLKVELKSFLEVLDGKIDDLHDFRRG
LSKLGTDN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199331
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199331.2, NP_955363.2
RefSeq Size 1807 bp
RefSeq ORF 866 bp
Locus ID 9455
Cytogenetics 15q25.2
Protein Families Druggable Genome
MW 32.7 kDa
Summary This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. The encoded protein is a postsynaptic density scaffolding protein. Alternative splicing results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 14. provided by RefSeq, Jun 2011
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Documents
Resources
"NM_199331" in other vectors (4)
SKU Description Size Price
RC214311L3 Lenti ORF clone of Human homer homolog 2 (Drosophila) (HOMER2), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC214311L4 Lenti ORF clone of Human homer homolog 2 (Drosophila) (HOMER2), transcript variant 3, mGFP tagged 10 ug
$600.00
RG214311 HOMER2 (tGFP-tagged) - Human homer homolog 2 (Drosophila) (HOMER2), transcript variant 3 10 ug
$500.00
SC317418 HOMER2 (untagged)-Human homer homolog 2 (Drosophila) (HOMER2), transcript variant 3 10 ug
$330.00

Plasmid Map for RC214311

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Cloning scheme for RC214311

Circular map for RC214311

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