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SMYD2 (NM_020197) Human Tagged ORF Clone

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SKU
RC214307
SMYD2 (Myc-DDK-tagged)-Human SET and MYND domain containing 2 (SMYD2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SMYD2
Synonyms HSKM-B; KMT3C; ZMYND14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214307 representing NM_020197
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGCCGAGGGCCTCGGCGGCCTGGAGCGCTTCTGCAGCCCGGGCAAAGGCCGGGGGCTGCGGGCTC
TGCAGCCCTTCCAGGTGGGGGACTTGCTGTTCTCCTGCCCGGCCTATGCCTACGTGCTCACGGTCAACGA
GCGGGGCAACCACTGCGAGTACTGCTTCACCAGGAAAGAAGGATTGTCCAAATGTGGAAGATGCAAGCAG
GCATTTTACTGCAATGTGGAGTGTCAGAAAGAAGATTGGCCCATGCACAAGCTGGAATGTTCTCCCATGG
TTGTTTTTGGGGAAAACTGGAATCCCTCGGAGACTGTAAGACTAACAGCAAGGATTCTGGCCAAACAGAA
AATCCACCCAGAGAGAACACCTTCGGAAAAATTGTTAGCTGTGAAGGAGTTTGAATCACATCTGGATAAG
TTAGACAATGAGAAGAAGGATTTGATTCAGAGTGACATAGCTGCTCTCCATCACTTTTACTCCAAGCATC
TCGAATTCCCTGACAATGATAGCCTCGTAGTACTCTTTGCACAGGTTAACTGTAATGGCTTCACAATTGA
AGATGAAGAACTTTCTCATTTGGGATCAGCGATATTTCCTGATGTTGCATTGATGAATCATAGCTGTTGC
CCCAATGTCATTGTGACCTACAAAGGGACCCTGGCAGAAGTCAGAGCTGTACAGGAAATCAAGCCGGGAG
AGGAGGTTTTTACCAGCTATATTGATCTCCTGTACCCAACGGAAGATAGAAATGACCGGTTAAGAGATTC
TTATTTCTTTACCTGTGAGTGCCAGGAGTGTACCACCAAGGACAAGGATAAGGCCAAGGTGGAAATCCGG
AAGCTCAGCGATCCCCCAAAGGCAGAAGCCATCCGAGACATGGTCAGATATGCACGCAACGTCATTGAAG
AGTTCCGGAGGGCCAAGCACTATAAATCCCCTAGTGAGCTGCTGGAGATCTGCGAGCTCAGCCAGGAGAA
GATGAGCTCTGTGTTTGAGGACAGTAACGTGTACATGTTGCACATGATGTACCAGGCCATGGGTGTCTGC
TTGTACATGCAGGACTGGGAAGGAGCCCTGCAATATGGACAGAAAATCATTAAGCCCTACAGTAAGCACT
ATCCTTTGTACTCCCTCAACGTGGCCTCCATGTGGTTGAAGCTAGGGAGACTCTACATGGGCCTGGAACA
CAAAGCCGCAGGGGAGAAAGCCCTGAAGAAGGCCATTGCAATCATGGAAGTAGCTCACGGCAAAGATCAT
CCATATATTTCTGAGATCAAACAGGAAATTGAAAGCCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214307 representing NM_020197
Red=Cloning site Green=Tags(s)

MRAEGLGGLERFCSPGKGRGLRALQPFQVGDLLFSCPAYAYVLTVNERGNHCEYCFTRKEGLSKCGRCKQ
AFYCNVECQKEDWPMHKLECSPMVVFGENWNPSETVRLTARILAKQKIHPERTPSEKLLAVKEFESHLDK
LDNEKKDLIQSDIAALHHFYSKHLEFPDNDSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCC
PNVIVTYKGTLAEVRAVQEIKPGEEVFTSYIDLLYPTEDRNDRLRDSYFFTCECQECTTKDKDKAKVEIR
KLSDPPKAEAIRDMVRYARNVIEEFRRAKHYKSPSELLEICELSQEKMSSVFEDSNVYMLHMMYQAMGVC
LYMQDWEGALQYGQKIIKPYSKHYPLYSLNVASMWLKLGRLYMGLEHKAAGEKALKKAIAIMEVAHGKDH
PYISEIKQEIESH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020197
ORF Size 1299 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020197.1, NP_064582.1
RefSeq Size 1694 bp
RefSeq ORF 1302 bp
Locus ID 56950
UniProt ID Q9NRG4
Cytogenetics 1q32.3
Domains SET, zf-MYND
Protein Families Druggable Genome
MW 49.5 kDa
Summary SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 PubMed 16805913).supplied by OMIM, Nov 2008
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"NM_020197" in other vectors (6)
SKU Description Size Price
RC214307L1 Lenti ORF clone of Human SET and MYND domain containing 2 (SMYD2), Myc-DDK-tagged 10 ug
$757.00
RC214307L2 Lenti ORF clone of Human SET and MYND domain containing 2 (SMYD2), mGFP tagged 10 ug
$757.00
RC214307L3 Lenti ORF clone of Human SET and MYND domain containing 2 (SMYD2), Myc-DDK-tagged 10 ug
$757.00
RC214307L4 Lenti ORF clone of Human SET and MYND domain containing 2 (SMYD2), mGFP tagged 10 ug
$757.00
RG214307 SMYD2 (tGFP-tagged) - Human SET and MYND domain containing 2 (SMYD2) 10 ug
$489.00 $657.00
SC113170 SMYD2 (untagged)-Human SET and MYND domain containing 2 (SMYD2) 10 ug
$457.00

Plasmid Map for RC214307

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214307
Circular map for RC214307

file-alt Citations

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