SMARCD2 (NM_001098426) Human Tagged ORF Clone

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SKU
RC214286
SMARCD2 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (SMARCD2)
  $494.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SMARCD2
Synonyms BAF60B; CRACD2; PRO2451; Rsc6p; SGD2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214286 representing NM_001098426
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGGCCGAGGCGCGGGCGGGTTCCCGCTGCCCCCGCTAAGCCCTGGCGGCGGCGCCGTGGCTGCGG
CCCTGGGAGCGCCGCCTCCCCCCGCGGGACCCGGCATGCTGCCCGGACCGGCGCTCCGGGGACCGGGTCC
GGCAGGAGGCGTGGGGGGCCCCGGGGCCGCCGCCTTCCGCCCCATGGGCCCCGCGGGCCCCGCGGCGCAG
TACCAGCGACCTGGCATGTCACCAGGGAACCGGATGCCCATGGCTGGCTTGCAGGTGGGACCCCCTGCTG
GCTCCCCATTTGGTGCAGCAGCTCCGCTTCGACCTGGCATGCCACCCACCATGATGGATCCATTCCGAAA
ACGCCTGCTTGTGCCCCAGGCGCAGCCTCCCATGCCTGCCCAGCGCCGGGGGTTAAAGAGGAGGAAGATG
GCAGATAAGGTTCTACCTCAGCGAATCCGGGAGCTTGTTCCAGAGTCTCAGGCGTACATGGATCTCTTGG
CTTTTGAGCGGAAGCTGGACCAGACCATTGCTCGCAAGCGGATGGAGATCCAGGAGGCCATCAAAAAGCC
TCTGACACAAAAGCGAAAGCTTCGGATCTACATTTCCAATACGTTCAGTCCCAGCAAGGCGGAAGGCGAT
AGTGCAGGAACTGCAGGGACCCCTGGGGGAACCCCAGCAGGGGACAAGGTGGCTTCCTGGGAACTCCGAG
TGGAAGGAAAACTGCTGGATGATCCTAGCAAACAGAAGAGGAAGTTTTCTTCATTCTTTAAGAGCCTCGT
CATTGAGCTGGACAAGGAGCTGTACGGGCCTGACAATCACCTGGTGGAGTGGCACCGGATGCCCACCACC
CAGGAGACAGATGGCTTCCAAGTAAAACGGCCTGGAGACCTCAACGTCAAGTGCACCCTCCTGCTCATGC
TGGATCATCAGCCTCCCCAGTACAAATTGGACCCCCGATTGGCAAGGCTGCTGGGAGTGCACACGCAGAC
GAGGGCCGCCATCATGCAGGCCCTGTGGCTTTACATCAAGCACAACCAGCTGCAGGATGGGCACGAGCGG
GAGTACATCAACTGCAACCGTTACTTCCGCCAGATCTTCAGTTGTGGCCGACTCCGTTTCTCCGAGATTC
CCATGAAGCTGGCAGGGTTGCTGCAGCATCCAGACCCCATTGTCATCAACCATGTCATTAGTGTCGACCC
TAACGACCAGAAGAAGACAGCCTGTTACGACATCGATGTGGAGGTGGACGACCCACTGAAGGCCCAAATG
AGCAATTTTCTGGCCTCTACCACCAATCAGCAGGAGATCGCCTCCCTTGATGTCAAGATCCATGAGACCA
TTGAGTCCATCAACCAGCTGAAGACCCAGAGAGATTTCATGCTCAGTTTTAGCACCGACCCCCAGGACTT
CATCCAGGAATGGCTCCGTTCCCAGCGCCGAGACCTCAAGATCATCACTGATGTGATTGGAAATCCTGAG
GAGGAGAGACGAGCTGCTTTCTACCACCAGCCCTGGGCCCAGGAAGCAGTAGGCAGGCACATCTTTGCCA
AGGTGCAGCAGCGAAGGCAGGAACTGGAACAGGTGCTGGGAATTCGCCTGACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214286 representing NM_001098426
Red=Cloning site Green=Tags(s)

MSGRGAGGFPLPPLSPGGGAVAAALGAPPPPAGPGMLPGPALRGPGPAGGVGGPGAAAFRPMGPAGPAAQ
YQRPGMSPGNRMPMAGLQVGPPAGSPFGAAAPLRPGMPPTMMDPFRKRLLVPQAQPPMPAQRRGLKRRKM
ADKVLPQRIRELVPESQAYMDLLAFERKLDQTIARKRMEIQEAIKKPLTQKRKLRIYISNTFSPSKAEGD
SAGTAGTPGGTPAGDKVASWELRVEGKLLDDPSKQKRKFSSFFKSLVIELDKELYGPDNHLVEWHRMPTT
QETDGFQVKRPGDLNVKCTLLLMLDHQPPQYKLDPRLARLLGVHTQTRAAIMQALWLYIKHNQLQDGHER
EYINCNRYFRQIFSCGRLRFSEIPMKLAGLLQHPDPIVINHVISVDPNDQKKTACYDIDVEVDDPLKAQM
SNFLASTTNQQEIASLDVKIHETIESINQLKTQRDFMLSFSTDPQDFIQEWLRSQRRDLKIITDVIGNPE
EERRAAFYHQPWAQEAVGRHIFAKVQQRRQELEQVLGIRLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001098426
ORF Size 1593 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001098426.2
RefSeq Size 2770 bp
RefSeq ORF 1596 bp
Locus ID 6603
UniProt ID Q92925
Cytogenetics 17q23.3
Protein Families Transcription Factors
MW 58.7 kDa
Summary The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001098426" in other vectors (4)
SKU Description Size Price
RC214286L3 Lenti-ORF clone of SMARCD2 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (SMARCD2) 10 ug
$794.00
RC214286L4 Lenti-ORF clone of SMARCD2 (mGFP-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (SMARCD2) 10 ug
$794.00
RG214286 SMARCD2 (tGFP-tagged) - Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (SMARCD2) 10 ug
$694.00
SC316240 SMARCD2 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2 (SMARCD2) 10 ug
$545.00

Plasmid Map for RC214286

Download sequence for

Cloning scheme for RC214286

Circular map for RC214286

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