THAP7 (NM_030573) Human Tagged ORF Clone

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SKU
RC214245
THAP7 (Myc-DDK-tagged)-Human THAP domain containing 7 (THAP7), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol THAP7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214245 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCGTCACTGCTCCGCCGCCGGCTGCTGCACACGGGACACGCGCGAGACGCGCAACCGCGGCATCT
CCTTCCACAGACTTCCCAAGAAGGACAACCCGAGGCGAGGCTTGTGGCTGGCCAACTGCCAGCGGCTGGA
CCCCAGCGGCCAGGGCCTGTGGGACCCGGCATCCGAGTACATCTACTTCTGCTCCAAACACTTTGAGGAG
GACTGCTTTGAGCTGGTGGGAATCAGTGGATATCACAGGCTAAAGGAGGGGGCAGTCCCCACCATATTTG
AGTCTTTCTCCAAGTTGCGCCGGACAACCAAGACCAAAGGACACAGTTACCCACCTGGCCCCCCTGAAGT
CAGCCGGCTCAGACGATGCAGGAAGCGCTGCTCCGAGGGCCGAGGGCCCACAACTCCATTTTCTCCACCT
CCACCTGCTGATGTCACCTGCTTTCCTGTGGAAGAGGCCTCAGCACCTGCCACTTTGCCGGCCTCCCCAG
CTGGGAGGCTGGAGCCTGGCCTTAGCAGCCCCTTTTCAGACCTACTGGGCCCCTTGGGTGCCCAGGCAGA
TGAAGCAGGCTGCAGCGCCCAGCCTTCACCAGAGCGGCAGCCCTCCCCTCTCGAACCACGGCCAGTCTCC
CCCTCAGCGTATATGCTGCGCCTGCCCCCACCCGCCGGAGCCTACATCCAGAATGAACACAGCTACCAGG
TGGGCAGCGCCTTACTCTGGAAGCGGCGAGCCGAGGCAGCCCTTGATGCCCTTGACAAGGCCCAGCGCCA
GCTGCAGGCCTGCAAGCGGCGGGAGCAGCGGCTGCGGTTGAGACTGACCAAGCTGCAGCAGGAGCGGGCA
CGGGAGAAGCGGGCACAGGCAGATGCCCGCCAGACTCTGAAGGAGCATGTGCAGGACTTTGCCATGCAGC
TGAGCAGCAGCATGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214245 protein sequence
Red=Cloning site Green=Tags(s)

MPRHCSAAGCCTRDTRETRNRGISFHRLPKKDNPRRGLWLANCQRLDPSGQGLWDPASEYIYFCSKHFEE
DCFELVGISGYHRLKEGAVPTIFESFSKLRRTTKTKGHSYPPGPPEVSRLRRCRKRCSEGRGPTTPFSPP
PPADVTCFPVEEASAPATLPASPAGRLEPGLSSPFSDLLGPLGAQADEAGCSAQPSPERQPSPLEPRPVS
PSAYMLRLPPPAGAYIQNEHSYQVGSALLWKRRAEAALDALDKAQRQLQACKRREQRLRLRLTKLQQERA
REKRAQADARQTLKEHVQDFAMQLSSSMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030573
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030573.1
RefSeq Size 1271 bp
RefSeq ORF 930 bp
Locus ID 80764
UniProt ID Q9BT49
Cytogenetics 22q11.21
Domains DM3
Protein Families Transcription Factors
MW 34.4 kDa
Summary Chromatin-associated, histone tail-binding protein that represses transcription via recruitment of HDAC3 and nuclear hormone receptor corepressors.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_030573" in other vectors (4)
SKU Description Size Price
RC214245L3 Lenti ORF clone of Human THAP domain containing 7 (THAP7), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC214245L4 Lenti ORF clone of Human THAP domain containing 7 (THAP7), transcript variant 1, mGFP tagged 10 ug
$600.00
RG214245 THAP7 (tGFP-tagged) - Human THAP domain containing 7 (THAP7), transcript variant 1 10 ug
$489.00 $500.00
SC109891 THAP7 (untagged)-Human THAP domain containing 7 (THAP7), transcript variant 1 10 ug
$300.00

Plasmid Map for RC214245

Download sequence for

Cloning scheme for RC214245

Circular map for RC214245

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