COPA (NM_001098398) Human Tagged ORF Clone
SKU
RC214199
COPA (Myc-DDK-tagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 1
$1,610.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | COPA |
| Synonyms | AILJK; alpha-COP; HEP-COP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001098398 |
| ORF Size | 3699 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001098398.1, NP_001091868.1 |
| RefSeq Size | 5666 bp |
| RefSeq ORF | 3702 bp |
| Locus ID | 1314 |
| UniProt ID | P53621 |
| Cytogenetics | 1q23.2 |
| MW | 139.1 kDa |
| Summary | In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP, encoded by COPA, shares high sequence similarity with RET1P, the alpha subunit of the coatomer complex in yeast. Also, the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide, xenin, which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001098398" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214199L1 | Lenti ORF clone of Human coatomer protein complex, subunit alpha (COPA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,910.00
|
|
| RC214199L2 | Lenti ORF clone of Human coatomer protein complex, subunit alpha (COPA), transcript variant 1, mGFP tagged | 10 ug |
$1,910.00
|
|
| RC214199L3 | Lenti ORF clone of Human coatomer protein complex, subunit alpha (COPA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,910.00
|
|
| RC214199L4 | Lenti ORF clone of Human coatomer protein complex, subunit alpha (COPA), transcript variant 1, mGFP tagged | 10 ug |
$1,910.00
|
|
| RG214199 | COPA (tGFP-tagged) - Human coatomer protein complex, subunit alpha (COPA), transcript variant 1 | 10 ug |
$1,810.00
|
|
| SC316290 | COPA (untagged)-Human coatomer protein complex, subunit alpha (COPA), transcript variant 1 | 10 ug |
$1,611.00
|
|
Citations
Deficiency in coatomer complex I causes aberrant activation of STING signalling
Steiner, Hrovat-Schaale, Prigione et al
Nat Commun (2022) 13 (1), 2321 DOI: 10.1038/s41467-022-29946-6
Steiner, Hrovat-Schaale, Prigione et al
Nat Commun (2022) 13 (1), 2321 DOI: 10.1038/s41467-022-29946-6
Cited by 100
AI Summary: OriGene’s pCMV6Entry-COPA-myc-DDK plasmid (Cat. No. RC214199) was used as a template to generate COPA syndrome patient mutations for functional studies investigating the role of COPI deficiency in aberrant STING signaling.
Snippet: g the COPA syndrome patient mutations c.721 G > A (p.E241K) and c.698 G > A (p.R233H)1 according to the manufacturer’s recommendations. As template, the pCMV6Entry-COPA-myc-DDK plasmid (Origene, Cat. No. RC214199, kind gift from A. Shum, Department of Medicine, University of California San Francisco) was used. Primer sequences are listed in Supplementary Table 2. Cell transfection
Mutations in COPA lead to abnormal trafficking of STING to the Golgi and interferon signaling
Lepelley, Martin-Niclós, Le Bihan et al
J. Exp. Med. (2020) 217 (11) DOI: 10.1084/jem.20200600
Lepelley, Martin-Niclós, Le Bihan et al
J. Exp. Med. (2020) 217 (11) DOI: 10.1084/jem.20200600
Cited by 225
Activation of STING due to COPI-deficiency
Steiner, Schaale, Prigione et al
bioRxiv (2020)
Steiner, Schaale, Prigione et al
bioRxiv (2020)
Cited by 18
COPA mutations impair ER-Golgi transport and cause hereditary autoimmune-mediated lung disease and arthritis
Watkin, Jessen, Wiszniewski et al
Nat. Genet. (2015)
Watkin, Jessen, Wiszniewski et al
Nat. Genet. (2015)
Cited by 408
AI Summary: OriGene’s pCMV6-Entry vectors encoding Myc-DDK–tagged or untagged human COPA were used as parental plasmids to generate wild-type and mutant COPA constructs for functional studies in this paper.
Snippet: … pCMV6-Entry vectors encoding Myc-DDK–tagged or untagged human COPA (Origene) were used as the parental vectors for mutant plasmid generation. …Cell lines stably expressing wild-type or Glu241Lys mutant COPA-Flag fusion protein in the presence of doxycycline were generated with the Flp-In T-REx system (Invitrogen). …
Showing 1-4 of 4 papers.
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