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VAC14 (NM_018052) Human Tagged ORF Clone

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SKU
RC214195
VAC14 (Myc-DDK-tagged)-Human Vac14 homolog (S. cerevisiae) (VAC14)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $716.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol VAC14
Synonyms ArPIKfyve; TAX1BP2; TRX
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214195 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCCCGAGAAGGATTTCGCGCCGCTCACGCCTAACATCGTGCGCGCCCTCAATGACAAGCTGTACG
AAAAGCGGAAGGTGGCAGCGCTGGAGATCGAGAAGCTGGTCCGGGAGTTCGTGGCCCAGAACAATACCGT
GCAAATCAAGCATGTGATCCAGACCCTGTCCCAGGAGTTTGCCCTGTCTCAGCACCCCCACAGCCGGAAA
GGGGGCCTCATCGGCCTGGCCGCCTGCTCCATCGCACTGGGCAAGGACTCAGGGCTCTACCTGAAGGAGC
TGATCGAGCCAGTGCTGACCTGCTTCAATGATGCAGACAGCAGGCTGCGCTACTATGCCTGCGAGGCCCT
CTACAACATCGTCAAGGTGGCCCGGGGCGCTGTGCTGCCCCACTTCAACGTGCTCTTTGACGGGCTGAGC
AAGCTGGCAGCCGACCCAGACCCCAATGTGAAAAGCGGATCTGAGCTCCTAGACCGCCTTTTAAAGGACA
TTGTGACTGAGAGCAACAAGTTTGACCTGGTGAGCTTCATCCCCTTGTTGCGAGAGAGGATTTACTCCAA
CAACCAGTATGCCCGGCAGTTCATCATCTCCTGGATCCTGGTTCTGGAGTCGGTGCCAGACATTAACCTG
CTGGATTACCTGCCGGAGATCCTGGATGGACTCTTCCAGATCCTGGGTGACAATGGCAAAGAGATTCGCA
AAATGTGTGAGGTTGTTCTTGGAGAATTCTTAAAAGAAATTAAGAAGAACCCCTCCAGTGTGAAGTTTGC
TGAGATGGCCAACATCCTGGTGATCCACTGCCAGACAACAGATGACCTCATCCAGCTGACAGCCATGTGC
TGGATGCGGGAGTTCATCCAGCTGGCGGGCCGCGTCATGCTGCCTTACTCCTCCGGGATCCTGACTGCTG
TCTTGCCCTGCTTGGCCTACGATGACCGCAAGAAAAGCATCAAAGAAGTGGCCAACGTGTGCAACCAGAG
CCTGATGAAGCTGGTCACCCCCGAGGACGACGAGCTGGATGAGCTGAGACCTGGGCAGAGGCAGGCAGAG
CCCACCCCTGACGATGCCCTGCCAAAGCAGGAGGGCACAGCCAGTGGAGGTCCAGATGGTTCCTGTGACT
CCAGCTTCAGTAGCGGCATCAGTGTCTTCACTGCAGCCAGCACTGAAAGAGCCCCAGTGACCCTTCACCT
CGACGGGATCGTGCAGGTCCTAAACTGCCACCTCAGTGACACGGCCATTGGGATGATGACCAGGATTGCA
GTTCTCAAGTGGCTCTACCACCTCTACATCAAAACTCCTCGGAAGATGTTCCGGCACACGGACAGCCTCT
TTCCCATCCTACTGCAGACGTTATCGGATGAATCGGATGAGGTGATCCTGAAGGACCTGGAGGTGCTGGC
AGAAATCGCTTCCTCCCCCGCAGGCCAGACGGATGACCCAGGCCCCCTCGATGGCCCTGACCTCCAGGCC
AGCCACTCAGAGCTCCAGGTGCCCACCCCTGGCAGAGCCGGCCTACTGAACACCTCTGGTACCAAAGGCT
TAGAATGTTCTCCTTCAACTCCCACCATGAATTCTTACTTTTATAAGTTCATGATCAACCTTCTCAAGAG
ATTCAGCAGCGAACGGAAGCTCCTGGAGGTCAGAGGCCCTTTCATCATCAGGCAGCTGTGCCTCCTGCTG
AATGCGGAGAACATCTTCCACTCAATGGCAGACATCCTGCTGCGGGAGGAGGACCTCAAGTTCGCCTCGA
CCATGGTCCACGCCCTCAACACCATCCTGCTGACCTCCACAGAGCTCTTCCAGCTAAGGAACCAGCTGAA
GGACCTGAAGACCCTGGAGAGCCAGAACCTGTTCTGCTGCCTGTACCGCTCCTGGTGCCACAACCCAGTC
ACCACGGTGTCCCTCTGCTTCCTCACCCAGAACTACCGGCACGCCTATGACCTCATCCAGAAGTTTGGGG
ACCTGGAGGTCACCGTGGACTTCCTCGCAGAGGTGGACAAGCTGGTGCAGCTGATTGAGTGCCCCATCTT
CACATATCTGCGCCTGCAGCTGCTGGACGTGAAGAACAACCCCTACCTGATCAAGGCCCTCTACGGCCTG
CTCATGCTCCTGCCGCAGAGCAGCGCCTTCCAGCTGCTCTCGCACCGGCTCCAGTGCGTGCCCAACCCTG
AGCTGCTGCAGACCGAAGACAGTCTAAAGGCAGCCCCCAAGTCCCAGAAAGCTGACTCCCCTAGCATCGA
CTACGCAGAGCTGCTGCAGCACTTTGAGAAGGTCCAGAACAAGCACCTGGAAGTGCGGCACCAGCGGAGC
GGGCGTGGGGACCACCTGGACCGGAGGGTTGTCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214195 protein sequence
Red=Cloning site Green=Tags(s)

MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVREFVAQNNTVQIKHVIQTLSQEFALSQHPHSRK
GGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLS
KLAADPDPNVKSGSELLDRLLKDIVTESNKFDLVSFIPLLRERIYSNNQYARQFIISWILVLESVPDINL
LDYLPEILDGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQLTAMC
WMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTPEDDELDELRPGQRQAE
PTPDDALPKQEGTASGGPDGSCDSSFSSGISVFTAASTERAPVTLHLDGIVQVLNCHLSDTAIGMMTRIA
VLKWLYHLYIKTPRKMFRHTDSLFPILLQTLSDESDEVILKDLEVLAEIASSPAGQTDDPGPLDGPDLQA
SHSELQVPTPGRAGLLNTSGTKGLECSPSTPTMNSYFYKFMINLLKRFSSERKLLEVRGPFIIRQLCLLL
NAENIFHSMADILLREEDLKFASTMVHALNTILLTSTELFQLRNQLKDLKTLESQNLFCCLYRSWCHNPV
TTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLAEVDKLVQLIECPIFTYLRLQLLDVKNNPYLIKALYGL
LMLLPQSSAFQLLSHRLQCVPNPELLQTEDSLKAAPKSQKADSPSIDYAELLQHFEKVQNKHLEVRHQRS
GRGDHLDRRVVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018052
ORF Size 2346 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018052.5
RefSeq Size 3107 bp
RefSeq ORF 2349 bp
Locus ID 55697
UniProt ID Q08AM6
Cytogenetics 16q22.1-q22.2
MW 88 kDa
Summary This gene encodes a scaffold protein that is a component of the PIKfyve protein kinase complex. This complex is responsible for the synthesis of phosphatidylinositol 3,5-bisphosphate, an important component of cellular membranes, from phosphatidylinositol 3-phosphate. Mice lacking a functional copy of this gene exhibit severe neurodegeneration. Mutations in the human gene have been identified in patients with a childhood onset progressive neurological disorder characterized by impaired movement, dystonia, and striatal abnormalities. provided by RefSeq, May 2017
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Documents
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"NM_018052" in other vectors (6)
SKU Description Size Price
RC214195L1 Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), Myc-DDK-tagged 10 ug
$1,016.00
RC214195L2 Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), mGFP tagged 10 ug
$1,016.00
RC214195L3 Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), Myc-DDK-tagged 10 ug
$1,016.00
RC214195L4 Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), mGFP tagged 10 ug
$1,016.00
RG214195 VAC14 (tGFP-tagged) - Human Vac14 homolog (S. cerevisiae) (VAC14) 10 ug
$916.00
SC314683 VAC14 (untagged)-Human Vac14 homolog (S. cerevisiae) (VAC14) 10 ug
$789.00

Plasmid Map for RC214195

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214195
Circular map for RC214195

file-alt Citations

Human genetic variation in VAC14 regulates Salmonella invasion and typhoid fever through modulation of cholesterol
Alvarez, Glover, Luo et al
PNAS (2017)
AI Summary: OriGene’s human VAC14mycDDK plasmid (OriGene clone #RC214195) was used in this study to experimentally assess the role of VAC14 expression in regulating cellular susceptibility to Salmonella invasion.
Snippet: Plasmids used included human VAC14mycDDK (OriGene clone #RC214195) and VAC14-gfp. BglII and XhoI were used to move VAC14-mycDDK into the BglII and SalI sites of pEGFP-N3 (Clontech)
Showing 1-1 of 1 papers.
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