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VAC14 (NM_018052) Human Untagged Clone

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SKU
SC314683
VAC14 (untagged)-Human Vac14 homolog (S. cerevisiae) (VAC14)
  $789.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol VAC14
Synonyms ArPIKfyve; TAX1BP2; TRX
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC314683 representing NM_018052.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAACCCCGAGAAGGATTTCGCGCCGCTCACGCCTAACATCGTGCGCGCCCTCAATGACAAGCTGTAC
GAAAAGCGGAAGGTGGCAGCGCTGGAGATCGAGAAGCTGGTCCGGGAGTTCGTGGCCCAGAACAATACC
GTGCAAATCAAGCATGTGATCCAGACCCTGTCCCAGGAGTTTGCCCTGTCTCAGCACCCCCACAGCCGG
AAAGGGGGCCTCATCGGCCTGGCCGCCTGCTCCATCGCACTGGGCAAGGACTCAGGGCTCTACCTGAAG
GAGCTGATCGAGCCAGTGCTGACCTGCTTCAATGATGCAGACAGCAGGCTGCGCTACTATGCCTGCGAG
GCCCTCTACAACATCGTCAAGGTGGCCCGGGGCGCTGTGCTGCCCCACTTCAACGTGCTCTTTGACGGG
CTGAGCAAGCTGGCAGCCGACCCAGACCCCAATGTGAAAAGCGGATCTGAGCTCCTAGACCGCCTTTTA
AAGGACATTGTGACTGAGAGCAACAAGTTTGACCTGGTGAGCTTCATCCCCTTGTTGCGAGAGAGGATT
TACTCCAACAACCAGTATGCCCGGCAGTTCATCATCTCCTGGATCCTGGTTCTGGAGTCGGTGCCAGAC
ATTAACCTGCTGGATTACCTGCCGGAGATCCTGGATGGACTCTTCCAGATCCTGGGTGACAATGGCAAA
GAGATTCGCAAAATGTGTGAGGTTGTTCTTGGAGAATTCTTAAAAGAAATTAAGAAGAACCCCTCCAGT
GTGAAGTTTGCTGAGATGGCCAACATCCTGGTGATCCACTGCCAGACAACAGATGACCTCATCCAGCTG
ACAGCCATGTGCTGGATGCGGGAGTTCATCCAGCTGGCGGGCCGCGTCATGCTGCCTTACTCCTCCGGG
ATCCTGACTGCTGTCTTGCCCTGCTTGGCCTACGATGACCGCAAGAAAAGCATCAAAGAAGTGGCCAAC
GTGTGCAACCAGAGCCTGATGAAGCTGGTCACCCCCGAGGACGACGAGCTGGATGAGCTGAGACCTGGG
CAGAGGCAGGCAGAGCCCACCCCTGACGATGCCCTGCCAAAGCAGGAGGGCACAGCCAGTGGAGGTCCA
GATGGTTCCTGTGACTCCAGCTTCAGTAGCGGCATCAGTGTCTTCACTGCAGCCAGCACTGAAAGAGCC
CCAGTGACCCTTCACCTCGACGGGATCGTGCAGGTCCTAAACTGCCACCTCAGTGACACGGCCATTGGG
ATGATGACCAGGATTGCAGTTCTCAAGTGGCTCTACCACCTCTACATCAAAACTCCTCGGAAGATGTTC
CGGCACACGGACAGCCTCTTTCCCATCCTACTGCAGACGTTATCGGATGAATCGGATGAGGTGATCCTG
AAGGACCTGGAGGTGCTGGCAGAAATCGCTTCCTCCCCCGCAGGCCAGACGGATGACCCAGGCCCCCTC
GATGGCCCTGACCTCCAGGCCAGCCACTCAGAGCTCCAGGTGCCCACCCCTGGCAGAGCCGGCCTACTG
AACACCTCTGGTACCAAAGGCTTAGAATGTTCTCCTTCAACTCCCACCATGAATTCTTACTTTTATAAG
TTCATGATCAACCTTCTCAAGAGATTCAGCAGCGAACGGAAGCTCCTGGAGGTCAGAGGCCCTTTCATC
ATCAGGCAGCTGTGCCTCCTGCTGAATGCGGAGAACATCTTCCACTCAATGGCAGACATCCTGCTGCGG
GAGGAGGACCTCAAGTTCGCCTCGACCATGGTCCACGCCCTCAACACCATCCTGCTGACCTCCACAGAG
CTCTTCCAGCTAAGGAACCAGCTGAAGGACCTGAAGACCCTGGAGAGCCAGAACCTGTTCTGCTGCCTG
TACCGCTCCTGGTGCCACAACCCAGTCACCACGGTGTCCCTCTGCTTCCTCACCCAGAACTACCGGCAC
GCCTATGACCTCATCCAGAAGTTTGGGGACCTGGAGGTCACCGTGGACTTCCTCGCAGAGGTGGACAAG
CTGGTGCAGCTGATTGAGTGCCCCATCTTCACATATCTGCGCCTGCAGCTGCTGGACGTGAAGAACAAC
CCCTACCTGATCAAGGCCCTCTACGGCCTGCTCATGCTCCTGCCGCAGAGCAGCGCCTTCCAGCTGCTC
TCGCACCGGCTCCAGTGCGTGCCCAACCCTGAGCTGCTGCAGACCGAAGACAGTCTAAAGGCAGCCCCC
AAGTCCCAGAAAGCTGACTCCCCTAGCATCGACTACGCAGAGCTGCTGCAGCACTTTGAGAAGGTCCAG
AACAAGCACCTGGAAGTGCGGCACCAGCGGAGCGGGCGTGGGGACCACCTGGACCGGAGGGTTGTCCTC
TGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_018052
Insert Size 2349 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018052.3
RefSeq Size 3107 bp
RefSeq ORF 2349 bp
Locus ID 55697
UniProt ID Q08AM6
Cytogenetics 16q22.1-q22.2
MW 88 kDa
Summary This gene encodes a scaffold protein that is a component of the PIKfyve protein kinase complex. This complex is responsible for the synthesis of phosphatidylinositol 3,5-bisphosphate, an important component of cellular membranes, from phosphatidylinositol 3-phosphate. Mice lacking a functional copy of this gene exhibit severe neurodegeneration. Mutations in the human gene have been identified in patients with a childhood onset progressive neurological disorder characterized by impaired movement, dystonia, and striatal abnormalities. provided by RefSeq, May 2017
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_018052" in other vectors (6)
SKU Description Size Price
RC214195 VAC14 (Myc-DDK-tagged)-Human Vac14 homolog (S. cerevisiae) (VAC14) 10 ug
$716.00
RC214195L1 Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), Myc-DDK-tagged 10 ug
$1,016.00
RC214195L2 Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), mGFP tagged 10 ug
$1,016.00
RC214195L3 Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), Myc-DDK-tagged 10 ug
$1,016.00
RC214195L4 Lenti ORF clone of Human Vac14 homolog (S. cerevisiae) (VAC14), mGFP tagged 10 ug
$1,016.00
RG214195 VAC14 (tGFP-tagged) - Human Vac14 homolog (S. cerevisiae) (VAC14) 10 ug
$916.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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