DBNDD2 (NM_001048223) Human Tagged ORF Clone

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SKU
RC214187
DBNDD2 (Myc-DDK-tagged)-Human dysbindin (dystrobrevin binding protein 1) domain containing 2 (DBNDD2), transcript variant 3
  $150.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DBNDD2
Synonyms C20orf35; CK1BP; HSMNP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214187 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCAAATCCTCGGGCCGCCCTGGAGCGCCAGCAGCTCCGCCTTCGGGAGCGGCAAAAATTCTTCG
AGGACATTTTACAGCCAGAGACAGAGTTTGTCTTTCCTCTGTCCCATCTGCATCTCGAGTCGCAGAGACC
CCCCATAGGTAGTATCTCATCCATGGAAGTGAATGTGGACACACTGGAGCAAGTAGAACTTATTGACCTT
GGGGACCCGGATGCAGCAGATGTGTTCTTGCCTTGCGAAGATCCTCCACCAACCCCCCAGTCGTCTGGGG
TGGACAACCATTTGGAGGAGCTGAGCCTGCCGGTGCCTACATCAGACAGGACCACATCTAGGACCTCCTC
CTCCTCCTCCTCCGACTCCTCCACCAACCTGCATAGCCCAAATCCAAGTGATGATGGAGCAGATACGCCC
TTGGCACAGTCGGATGAAGAGGAGGAAAGGGGTGATGGAGGGGCAGAGCCTGGAGCCTGCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214187 protein sequence
Red=Cloning site Green=Tags(s)

MDPNPRAALERQQLRLRERQKFFEDILQPETEFVFPLSHLHLESQRPPIGSISSMEVNVDTLEQVELIDL
GDPDAADVFLPCEDPPPTPQSSGVDNHLEELSLPVPTSDRTTSRTSSSSSSDSSTNLHSPNPSDDGADTP
LAQSDEEEERGDGGAEPGACS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001048223
ORF Size 483 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001048223.3
RefSeq Size 1114 bp
RefSeq ORF 486 bp
Locus ID 55861
UniProt ID Q9BQY9, Q9BQY9-2
Cytogenetics 20q13.12
MW 17.5 kDa
Summary May modulate the activity of casein kinase-1. Inhibits CSNK1D autophosphorylation (in vitro).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001048223" in other vectors (4)
SKU Description Size Price
RC214187L3 Lenti ORF clone of Human dysbindin (dystrobrevin binding protein 1) domain containing 2 (DBNDD2), transcript variant 3, Myc-DDK-tagged 10 ug
$450.00
RC214187L4 Lenti ORF clone of Human dysbindin (dystrobrevin binding protein 1) domain containing 2 (DBNDD2), transcript variant 3, mGFP tagged 10 ug
$450.00
RG214187 DBNDD2 (tGFP-tagged) - Human dysbindin (dystrobrevin binding protein 1) domain containing 2 (DBNDD2), transcript variant 3 10 ug
$350.00
SC315404 DBNDD2 (untagged)-Human dysbindin (dystrobrevin binding protein 1) domain containing 2 (DBNDD2), transcript variant 3 10 ug
$165.00

Plasmid Map for RC214187

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Cloning scheme for RC214187

Circular map for RC214187

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