KRT33A (NM_004138) Human Tagged ORF Clone

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SKU
RC214177
KRT33A (Myc-DDK-tagged)-Human keratin 33A (KRT33A)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRT33A
Synonyms Ha-3I; HA3I; hHa3-I; K33A; Krt1-3; KRTHA3A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214177 representing NM_004138
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTACAGTTGTGGCCTGCCCAGCCTGAGCTGCCGCACCAGCTGCTCCTCCCGGCCCTGTGTGCCCC
CCAGCTGCCACGGCTGCACCCTGCCCGGGGCCTGCAACATCCCCGCCAATGTGAGCAACTGCAACTGGTT
CTGTGAGGGCTCCTTCAATGGCAGTGAGAAGGAGACCATGCAGTTCCTGAACGACCGCCTGGCCAGCTAC
CTGGAGAAGGTGCGTCAGCTGGAGCGGGACAACGCGGAGCTGGAGAACCTCATCCGGGAGCGGTCACAGC
AGCAGGAGCCCTTGGTGTGTGCCAGCTACCAGTCCTACTTCAAGACCATTGAGGAGCTCCAGCAGAAGAT
CCTGTGCAGCAAGTCTGAGAATGCCAGGCTTGTGGTGCAGATCGACAATGCCAAGCTGGCCTCAGATGAC
TTCAGGACCAAATATGAGACCGAGCTGTCCCTGCGGCAGCTGGTGGAGTCGGACATCAATGGCCTGCGCA
GGATCCTGGATGAGCTGACCCTGTGCAGGTCTGACCTGGAGGCCCAGGTGGAGTCCCTGAAGGAGGAGCT
GCTGTGCCTCAAGCAGAACCATGAGCAGGAGGTTAACACCCTGCGCTGCCAGCTTGGAGACCGCCTCAAC
GTGGAGGTGGACGCTGCTCCCACTGTGGACCTGAACCAGGTCCTGAATGAGACCAGGAGTCAGTATGAGG
CCCTGGTGGAAACCAACCGCAGGGAAGTGGAGCAATGGTTCGCCACGCAGACCGAGGAGCTGAACAAGCA
GGTGGTATCCAGCTCGGAGCAGCTGCAGTCCTACCAGGCGGAGATCATCGAGCTGAGACGCACGGTCAAT
GCCCTGGAGATCGAGCTGCAGGCCCAGCACAACCTGCGAGACTCTCTGGAAAACACGCTGACAGAGAGCG
AGGCCCGCTACAGCTCCCAGCTGTCCCAGGTGCAGAGACTGATCACCAACGTGGAGTCCCAGCTGGCGGA
GATCCGCAGTGACCTGGAGCGGCAGAACCAGGAGTATCAGGTGCTGCTGGACGTGCGGGCGCGGCTGGAG
TGTGAGATCAACACGTACCGGAGCCTGCTGGAGAGCGAGGACTGCAAGCTCCCCTCCAACCCCTGCGCCA
CAACCAATGCATGTGACAAGTCCACTGGGCCCTGTATCTCTAATCCCTGTGGCCTACGTGCTCGGTGTGG
GCCTTGCAACACATTTGGGTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214177 representing NM_004138
Red=Cloning site Green=Tags(s)

MSYSCGLPSLSCRTSCSSRPCVPPSCHGCTLPGACNIPANVSNCNWFCEGSFNGSEKETMQFLNDRLASY
LEKVRQLERDNAELENLIRERSQQQEPLVCASYQSYFKTIEELQQKILCSKSENARLVVQIDNAKLASDD
FRTKYETELSLRQLVESDINGLRRILDELTLCRSDLEAQVESLKEELLCLKQNHEQEVNTLRCQLGDRLN
VEVDAAPTVDLNQVLNETRSQYEALVETNRREVEQWFATQTEELNKQVVSSSEQLQSYQAEIIELRRTVN
ALEIELQAQHNLRDSLENTLTESEARYSSQLSQVQRLITNVESQLAEIRSDLERQNQEYQVLLDVRARLE
CEINTYRSLLESEDCKLPSNPCATTNACDKSTGPCISNPCGLRARCGPCNTFGY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004138
ORF Size 1212 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004138.4
RefSeq Size 1252 bp
RefSeq ORF 1215 bp
Locus ID 3883
UniProt ID O76009
Cytogenetics 17q21.2
MW 45.8 kDa
Summary This gene encodes a member of the keratin gene family. This gene is one of multiple type I hair keratin genes that are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, the encoded protein is an acidic protein which heterodimerizes with type II keratins to form hair and nails. There are two isoforms of this protein, encoded by two separate genes, keratin 33A and keratin 33B. provided by RefSeq, May 2012
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Documents
Resources
"NM_004138" in other vectors (4)
SKU Description Size Price
RC214177L3 Lenti ORF clone of Human keratin 33A (KRT33A), Myc-DDK-tagged 10 ug
$757.00
RC214177L4 Lenti ORF clone of Human keratin 33A (KRT33A), mGFP tagged 10 ug
$757.00
RG214177 KRT33A (tGFP-tagged) - Human keratin 33A (KRT33A) 10 ug
$657.00
SC303439 KRT33A (untagged)-Human keratin 33A (KRT33A) 10 ug
$503.00

Plasmid Map for RC214177

Download sequence for

Cloning scheme for RC214177

Circular map for RC214177

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