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PAK5 (NM_177990) Human Tagged ORF Clone

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SKU
RC214173
PAK7 (Myc-DDK-tagged)-Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $658.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PAK5 or NM_177990"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PAK5
Synonyms PAK7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214173 representing NM_177990
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGGGAAGAAAAAGAAAAAGATTGAAATATCTGGCCCGTCCAACTTTGAACACAGGGTTCATACTG
GGTTTGATCCACAAGAGCAGAAGTTTACCGGCCTTCCCCAGCAGTGGCACAGCCTGTTAGCAGATACGGC
CAACAGGCCAAAGCCTATGGTGGACCCTTCATGCATCACACCCATCCAGCTGGCTCCTATGAAGACAATC
GTTAGAGGAAACAAACCCTGCAAGGAAACCTCCATCAACGGCCTGCTAGAGGATTTTGACAACATCTCGG
TGACTCGCTCCAACTCCCTAAGGAAAGAAAGCCCACCCACCCCAGATCAGGGAGCCTCCAGCCACGGTCC
AGGCCACGCGGAAGAAAATGGCTTCATCACCTTCTCCCAGTATTCCAGCGAATCCGATACTACTGCTGAC
TACACGACCGAAAAGTACAGGGAGAAGAGTCTCTATGGAGATGATCTGGATCCGTATTATAGAGGCAGCC
ACGCAGCCAAGCAAAATGGGCACGTAATGAAAATGAAGCACGGGGAGGCCTACTATTCTGAGGTGAAGCC
TTTGAAATCCGATTTTGCCAGATTTTCTGCCGATTATCACTCACATTTGGACTCACTGAGCAAACCAAGT
GAATACAGTGACCTCAAGTGGGAGTATCAGAGAGCCTCGAGTAGCTCCCCTCTGGATTATTCATTCCAAT
TCACACCTTCTAGAACTGCAGGGACCAGCGGGTGCTCCAAGGAGAGCCTGGCGTACAGTGAAAGTGAATG
GGGACCCAGCCTGGATGACTATGACAGGAGGCCAAAGTCTTCGTACCTGAATCAGACAAGCCCTCAGCCC
ACCATGCGGCAGAGGTCCAGGTCAGGCTCGGGACTCCAGGAACCGATGATGCCATTTGGAGCAAGTGCAT
TTAAAACCCATCCCCAAGGACACTCCTACAACTCCTACACCTACCCTCGCTTGTCCGAGCCCACAATGTG
CATTCCAAAGGTGGATTACGATCGAGCACAGATGGTCCTCAGCCCTCCACTGTCAGGGTCTGACACCTAC
CCCAGGGGCCCTGCCAAACTACCTCAAAGTCAAAGCAAATCGGGCTATTCCTCAAGCAGTCACCAGTACC
CGTCTGGGTACCACAAAGCCACCTTGTACCATCACCCCTCCCTGCAGAGCAGTTCGCAGTACATCTCCAC
GGCTTCCTACCTGAGCTCCCTCAGCCTCTCATCCAGCACCTACCCGCCGCCCAGCTGGGGCTCCTCCTCC
GACCAGCAGCCCTCCAGGGTGTCCCATGAACAGTTTCGGGCGGCCCTGCAGCTGGTGGTCAGCCCAGGAG
ACCCCAGGGAATACTTGGCCAACTTTATCAAAATCGGGGAAGGCTCAACCGGCATCGTATGCATCGCCAC
CGAGAAACACACAGGGAAACAAGTTGCAGTGAAGAAAATGGACCTCCGGAAGCAACAGAGACGAGAATTG
CTTTTCAATGAGGTCGTGATCATGCGGGATTACCACCATGACAATGTGGTTGACATGTACAACAGCTACC
TTGTCGGCGATGAGCTCTGGGTGGTCATGGAGTTTCTAGAAGGTGGTGCCTTGACAGACATTGTGACTCA
CACCAGAATGAATGAAGAACAGATAGCTACTGTCTGCCTGTCAGTTCTGAGAGCTCTCTCCTACCTTCAT
AACCAAGGAGTGATTCACAGGGACATAAAAAGTGACTCCATCCTCCTGACAAGCGATGGCCGGATAAAGT
TGTCTGATTTTGGTTTCTGTGCTCAAGTTTCCAAAGAGGTGCCGAAGAGGAAATCATTGGTTGGCACTCC
CTACTGGATGGCCCCTGAGGTGATTTCTAGGCTACCTTATGGGACAGAGGTGGACATCTGGTCCCTCGGG
ATCATGGTGATAGAAATGATTGATGGCGAGCCCCCCTACTTCAATGAGCCTCCCCTCCAGGCGATGCGGA
GGATCCGGGACAGTTTACCTCCAAGAGTGAAGGACCTACACAAGGTTTCTTCAGTGCTCCGGGGATTCCT
AGACTTGATGTTGGTGAGGGAGCCCTCTCAGAGAGCAACAGCCCAGGAACTCCTCGGACATCCATTCTTA
AAACTAGCAGGTCCACCGTCTTGCATCGTCCCCCTCATGAGACAATACAGGCATCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214173 representing NM_177990
Red=Cloning site Green=Tags(s)

MFGKKKKKIEISGPSNFEHRVHTGFDPQEQKFTGLPQQWHSLLADTANRPKPMVDPSCITPIQLAPMKTI
VRGNKPCKETSINGLLEDFDNISVTRSNSLRKESPPTPDQGASSHGPGHAEENGFITFSQYSSESDTTAD
YTTEKYREKSLYGDDLDPYYRGSHAAKQNGHVMKMKHGEAYYSEVKPLKSDFARFSADYHSHLDSLSKPS
EYSDLKWEYQRASSSSPLDYSFQFTPSRTAGTSGCSKESLAYSESEWGPSLDDYDRRPKSSYLNQTSPQP
TMRQRSRSGSGLQEPMMPFGASAFKTHPQGHSYNSYTYPRLSEPTMCIPKVDYDRAQMVLSPPLSGSDTY
PRGPAKLPQSQSKSGYSSSSHQYPSGYHKATLYHHPSLQSSSQYISTASYLSSLSLSSSTYPPPSWGSSS
DQQPSRVSHEQFRAALQLVVSPGDPREYLANFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRREL
LFNEVVIMRDYHHDNVVDMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATVCLSVLRALSYLH
NQGVIHRDIKSDSILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSLG
IMVIEMIDGEPPYFNEPPLQAMRRIRDSLPPRVKDLHKVSSVLRGFLDLMLVREPSQRATAQELLGHPFL
KLAGPPSCIVPLMRQYRHH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177990
ORF Size 2157 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177990.4
RefSeq Size 4506 bp
RefSeq ORF 2160 bp
Locus ID 57144
UniProt ID Q9P286, B0AZM9
Cytogenetics 20p12.2
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
MW 80.6 kDa
Summary The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_177990" in other vectors (7)
SKU Description Size Price
RC214173L1 Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2, Myc-DDK-tagged 10 ug
$958.00
RC214173L2 Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2, mGFP tagged 10 ug
$958.00
RC214173L3 Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2, Myc-DDK-tagged 10 ug
$958.00
RC214173L4 Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2, mGFP tagged 10 ug
$958.00
RG214173 PAK7 (tGFP-tagged) - Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2 10 ug
$789.00 $858.00
SC107077 PAK7 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2 10 ug
$659.00
SC323605 PAK7 (untagged)-Kinase deficient mutant (K478M) of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2 10 ug
$659.00

Plasmid Map for RC214173

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214173
Circular map for RC214173

file-alt Citations

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