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PAK5 (NM_177990) Human Untagged Clone

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SKU
SC323605
PAK7 (untagged)-Kinase deficient mutant (K478M) of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2
  $659.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PAK5
Synonyms PAK7
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_177990, the custom clone sequence may differ by one or more nucleotides


ATGTTTGGGAAGAAAAAGAAAAAGATTGAAATATCTGGCCCGTCCAACTTTGAACACAGGGTTCATACTG
GGTTTGATCCACAAGAGCAGAAGTTTACCGGCCTTCCCCAGCAGTGGCACAGCCTGTTAGCAGATACGGC
CAACAGGCCAAAGCCTATGGTGGACCCTTCATGCATCACACCCATCCAGCTGGCTCCTATGAAGACAATC
GTTAGAGGAAACAAACCCTGCAAGGAAACCTCCATCAACGGCCTGCTAGAGGATTTTGACAACATCTCGG
TGACTCGCTCCAACTCCCTAAGGAAAGAAAGCCCACCCACCCCAGATCAGGGAGCCTCCAGCCACGGTCC
AGGCCACGCGGAAGAAAATGGCTTCATCACCTTCTCCCAGTATTCCAGCGAATCCGATACTACTGCTGAC
TACACGACCGAAAAGTACAGGGAGAAGAGTCTCTATGGAGATGATCTGGATCCGTATTATAGAGGCAGCC
ACGCAGCCAAGCAAAATGGGCACGTAATGAAAATGAAGCACGGGGAGGCCTACTATTCTGAGGTGAAGCC
TTTGAAATCCGATTTTGCCAGATTTTCTGCCGATTATCACTCACATTTGGACTCACTGAGCAAACCAAGT
GAATACAGTGACCTCAAGTGGGAGTATCAGAGAGCCTCGAGTAGCTCCCCTCTGGATTATTCATTCCAAT
TCACACCTTCTAGAACTGCAGGGACCAGCGGGTGCTCCAAGGAGAGCCTGGCGTACAGTGAAAGTGAATG
GGGACCCAGCCTGGATGACTATGACAGGAGGCCAAAGTCTTCGTACCTGAATCAGACAAGCCCTCAGCCC
ACCATGCGGCAGAGGTCCAGGTCAGGCTCGGGACTCCAGGAACCGATGATGCCATTTGGAGCAAGTGCAT
TTAAAACCCATCCCCAAGGACACTCCTACAACTCCTACACCTACCCTCGCTTGTCCGAGCCCACAATGTG
CATTCCAAAGGTGGATTACGATCGAGCACAGATGGTCCTCAGCCCTCCACTGTCAGGGTCTGACACCTAC
CCCAGGGGCCCTGCCAAACTACCTCAAAGTCAAAGCAAATCGGGCTATTCCTCAAGCAGTCACCAGTACC
CGTCTGGGTACCACAAAGCCACCTTGTACCATCACCCCTCCCTGCAGAGCAGTTCGCAGTACATCTCCAC
GGCTTCCTACCTGAGCTCCCTCAGCCTCTCATCCAGCACCTACCCGCCGCCCAGCTGGGGCTCCTCCTCC
GACCAGCAGCCCTCCAGGGTGTCCCATGAACAGTTTCGGGCGGCCCTGCAGCTGGTGGTCAGCCCAGGAG
ACCCCAGGGAATACTTGGCCAACTTTATCAAAATCGGGGAAGGCTCAACCGGCATCGTATGCATCGCCAC
CGAGAAACACACAGGGAAACAAGTTGCAGTGAAGAAAATGGACCTCCGGAAGCAACAGAGACGAGAACTG
CTTTTCAATGAGGTCGTGATCATGCGGGATTACCACCATGACAATGTGGTTGACATGTACAGCAGCTACC
TTGTCGGCGATGAGCTCTGGGTGGTCATGGAGTTTCTAGAAGGTGGTGCCTTGACAGACATTGTGACTCA
CACCAGAATGAATGAAGAACAGATAGCTACTGTCTGCCTGTCAGTTCTGAGAGCTCTCTCCTACCTTCAT
AACCAAGGAGTGATTCACAGGGACATAAAAAGTGACTCCATCCTCCTGACAAGCGATGGCCGGATAAAGT
TGTCTGATTTTGGTTTCTGTGCTCAAGTTTCCAAAGAGGTGCCGAAGAGGAAATCATTGGTTGGCACTCC
CTACTGGATGGCCCCTGAGGTGATTTCTAGGCTACCTTATGGGACAGAGGTGGACATCTGGTCCCTCGGG
ATCATGGTGATAGAAATGATTGATGGCGAGCCCCCCTACTTCAATGAGCCTCCCCTCCAGGCGATGCGGA
GGATCCGGGACAGTTTACCTCCAAGAGTGAAGGACCTACACAAGGTTTCTTCAGTGCTCCGGGGATTCCT
AGACTTGATGTTGGTGAGGGAGCCCTCTCAGAGAGCAACAGCCCAGGAACTCCTCGGACATCCATTCTTA
AAACTAGCAGGTCCACCGTCTTGCATCGTCCCCCTCATGAGACAATACAGGCATCACTGA

5' Read Nucleotide Sequence
>OriGene 5' read for mutant NM_177990 unedited
CCGCCTCGTTCCAGCAAAGGGCGGTAGGCGCTGTACGGTTGGGAGGTCTATATAAGCAGAGCTCGTTTAG
TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCCCTTGCC
CCTACCCAGCCAGTAGTAGTTCCCCAGCGTGCGCCCGGGGAGACCGGGAACATGGCGCTGGGAGCGCTGT
AGCAGCTGAGAAGGGGCTGAGGCACCGCCGCTTCGCTGACAGCCGGCCACCAGAAATACACTGTGGTGAA
ATGCTTCCACCTCTTGCTAAAATGAACACTGAAAAAAAATGAAGAAGACTGACAGCCCCCACCGAAGAGT
GTGGGGGAGTAGAAATACCCCCCTCCTTCTGGAGTCTTTATTTCATCCCCCGGCCATCATATAAGGGTTT
TTGCATCATGGTTGGGAAAAAAAAAAAAAAATTGAATATCGGGCGCTCCAAATTGGAAAAGGGTTCACTG
GGTTGTTCCAAAAACAAACTTTACCAGCCCTTACAGAAATGGCCACC
Kinase Domain Sequence
>SC323605 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CTTAWCAATCGGGGAGGCTCACCGGCATCGTATGCATCGCCACCGAGAAACACACAGGGAAACAAGTTGC
AGTGATGAAAATGGACCTCCGGAAGCAACAGAGACGAGAATTGCTTTTCAATGAGGTCGTGATCATGCGG
GATTACCACCATGACAATGTGGTTGACATGTACAACAGCTACCTTGTCGGCGATGAGCTCTGGGTGGTCA
TGGAGTTTCTAGAAGGTGGTGCCTTGACAGACATTGTGACTCACA
Restriction Sites Please inquire |
ACCN NM_177990
Insert Size 4840 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177990.1, NP_817127.1
RefSeq Size 4506 bp
RefSeq ORF 2160 bp
Locus ID 57144
UniProt ID Q9P286
Cytogenetics 20p12.2
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
Summary The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) lacks an internal 5' UTR exon, as compared to variant 1. Both variants encode the same protein.
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"NM_177990" in other vectors (7)
SKU Description Size Price
RC214173 PAK7 (Myc-DDK-tagged)-Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2 10 ug
$658.00
RC214173L1 Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2, Myc-DDK-tagged 10 ug
$958.00
RC214173L2 Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2, mGFP tagged 10 ug
$958.00
RC214173L3 Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2, Myc-DDK-tagged 10 ug
$958.00
RC214173L4 Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2, mGFP tagged 10 ug
$958.00
RG214173 PAK7 (tGFP-tagged) - Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2 10 ug
$789.00 $858.00
SC107077 PAK7 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2 10 ug
$659.00

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