OR7C2 (NM_012377) Human Tagged ORF Clone

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SKU
RC214145
OR7C2 (Myc-DDK-tagged)-Human olfactory receptor, family 7, subfamily C, member 2 (OR7C2)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OR7C2
Synonyms CIT-HSP-87M17; OR7C3; OR19-18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214145 representing NM_012377
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAGAGGAAACCAAACAGAAGTTGGAAACTTTCTCCTCCTGGGATTCGCAGAGGACTCTGACATGC
AGCTTCTCCTCCATGGGCTGTTCCTCTCCATGTACCTGGTTACCATCATCGGAAACCTGCTCATCATCCT
GACCATCAGTTCAGACTCCCACCTCCACACCCCCATGTACTTCTTCCTCTCCAACCTGTCCTTTGCTGAC
ATCTGTTTCACATCCACGACTGTCCCAAAGATGCTGGTGAATATCCAAACACAAAGCAAAATGATCACTT
TTGCAGGCTGCCTCACTCAGATATTTTTTTTCATTGCATTTGGATGCCTGGACAATTTGCTCCTGACCAT
GACGGCCTATGACCGCTTCGTGGCCATCTGTTACCCCCTGCACTACACGGTCATCATGAACCCCCGGCTC
TGTGGACTGCTGGTTCTGGGGTCCTGGTGCATCAGTGTCATGGGTTCCTTGCTTGAGACCTTGACCATTT
TGAGGCTGTCCTTCTGCACAAATATGGAAATTCCGCACTTTTTTTGTGATCCTTCCGAAGTCCTGAAGCT
GGCCTGTTCTGACACCTTCATCAATAACATCGTGATGTATTTTGTGACCATTGTCCTGGGTGTTTTTCCT
CTCTGTGGAATCCTATTCTCTTATTCTCAGATTTTCTCCTCCGTCCTAAGAGTATCTGCCAGAGGCCAGC
ACAAAGCCTTTTCCACCTGTGGTTCCCACCTCTCAGTGGTCAGCTTGTTCTATGGCACTGGCCTTGGGGT
CTATCTCAGTTCTGCAGTTACACCACCTTCTAGGACAAGTCTGGCAGCCTCGGTGATGTACACCATGGTC
ACCCCCATGCTGAACCCCTTCATCTACAGCCTGAGGAACAAGGACATGAAGGGGTCACTGGGGAGACTCC
TCCTCAGGGCAACGTCTCTCAAAGAGGGGACCATTGCTAAGCTCTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214145 representing NM_012377
Red=Cloning site Green=Tags(s)

MERGNQTEVGNFLLLGFAEDSDMQLLLHGLFLSMYLVTIIGNLLIILTISSDSHLHTPMYFFLSNLSFAD
ICFTSTTVPKMLVNIQTQSKMITFAGCLTQIFFFIAFGCLDNLLLTMTAYDRFVAICYPLHYTVIMNPRL
CGLLVLGSWCISVMGSLLETLTILRLSFCTNMEIPHFFCDPSEVLKLACSDTFINNIVMYFVTIVLGVFP
LCGILFSYSQIFSSVLRVSARGQHKAFSTCGSHLSVVSLFYGTGLGVYLSSAVTPPSRTSLAASVMYTMV
TPMLNPFIYSLRNKDMKGSLGRLLLRATSLKEGTIAKLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012377
ORF Size 957 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012377.1, NP_036509.1
RefSeq Size 960 bp
RefSeq ORF 960 bp
Locus ID 26658
UniProt ID O60412
Cytogenetics 19p13.12
Protein Families Druggable Genome, Transmembrane
Protein Pathways Olfactory transduction
MW 35.1 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_012377" in other vectors (4)
SKU Description Size Price
RC214145L3 Lenti ORF clone of Human olfactory receptor, family 7, subfamily C, member 2 (OR7C2), Myc-DDK-tagged 10 ug
$600.00
RC214145L4 Lenti ORF clone of Human olfactory receptor, family 7, subfamily C, member 2 (OR7C2), mGFP tagged 10 ug
$600.00
RG214145 OR7C2 (tGFP-tagged) - Human olfactory receptor, family 7, subfamily C, member 2 (OR7C2) 10 ug
$500.00
SC303972 OR7C2 (untagged)-Human olfactory receptor, family 7, subfamily C, member 2 (OR7C2) 10 ug
$330.00

Plasmid Map for RC214145

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Cloning scheme for RC214145

Circular map for RC214145

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