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USP47 (NM_017944) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC214048
USP47 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 47 (USP47)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$589.00 $1,120.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol USP47
Synonyms TRFP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214048 representing NM_017944
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAAGAGGCACCACTGGATCATACCAGTGACAAGTCAC
TTCTCGACGCTAATTTTGAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGTGAACAACC
TCAAATACTGCTGGAGGATTCCAGTGCTGGGGAAGACAGTGTTCATGACAGGTTTATAGGTCCGCTTCCA
AGAGAAGGTTCTGGGGGTTCTACCAGTGATTATGTCAGCCAAAGCTACTCCTACTCATCTATTTTGAATA
AATCAGAAACTGGATATGTGGGACTAGTAAACCAAGCAATGACTTGCTATTTGAATAGCCTTTTGCAAAC
ACTTTTTATGACTCCTGAATTTAGGAATGCATTATATAAGTGGGAATTTGAAGAATCTGAAGAAGATCCA
GTGACAAGTATTCCATACCAACTTCAAAGGCTTTTTGTTTTGTTACAAACCAGCAAAAAGAGAGCAATTG
AAACCACAGATGTTACAAGGAGCTTTGGATGGGATAGTAGTGAGGCTTGGCAGCAGCATGATGTACAAGA
ACTATGCAGAGTCATGTTTGATGCTTTGGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAAT
GAGCTATATCAAGGCAAGCTGAAGGACTACGTGAGATGTCTGGAATGTGGTTATGAGGGCTGGCGAATCG
ACACATATCTTGATATTCCATTGGTCATCCGACCTTATGGGTCCAGCCAAGCATTTGCTAGTGTGGAAGA
AGCATTGCATGCATTTATTCAGCCAGAGATTCTGGATGGCCCAAATCAGTATTTTTGTGAACGTTGTAAG
AAGAAGTGTGATGCACGGAAGGGCCTTCGGTTTTTGCATTTTCCTTATCTGCTGACCTTACAGCTGAAAA
GATTCGATTTTGATTATACAACCATGCATAGGATTAAACTGAATGATCGAATGACATTTCCCGAGGAACT
AGATATGAGTACTTTTATTGATGTTGAAGATGAGAAATCTCCTCAGACTGAAAGTTGCACTGACAGTGGA
GCAGAAAATGAAGGTAGTTGTCACAGTGATCAGATGAGCAACGATTTCTCCAATGATGATGGTGTTGATG
AAGGAATCTGTCTTGAAACCAATAGTGGAACTGAAAAGATCTCAAAATCTGGACTTGAAAAGAATTCCTT
GATCTATGAACTTTTCTCTGTTATGGTTCATTCTGGGAGCGCTGCTGGTGGTCATTATTATGCATGTATA
AAGTCATTCAGTGATGAGCAGTGGTACAGCTTCAATGATCAACATGTCAGCAGGATAACACAAGAGGACA
TTAAGAAAACACATGGTGGATCTTCAGGAAGCAGAGGATATTATTCTAGTGCTTTCGCAAGTTCCACAAA
TGCATATATGCTGATCTATAGACTGAAGGATCCAGCCAGAAATGCAAAATTTCTAGAAGTGGATGAATAC
CCAGAACATATTAAAAACTTGGTGCAGAAAGAGAGAGAGTTGGAAGAACAAGAAAAGAGACAACGAGAAA
TTGAGCGCAATACATGCAAGATAAAATTATTCTGTTTGCATCCTACAAAACAAGTAATGATGGAAAATAA
ATTGGAGGTTCATAAGGATAAGACATTAAAGGAAGCAGTAGAAATGGCTTATAAGATGATGGATTTAGAA
GAGGTAATACCCCTGGATTGCTGTCGCCTTGTTAAATATGATGAGTTTCATGATTATCTAGAACGGTCAT
ATGAAGGAGAAGAAGATACACCAATGGGGCTTCTACTAGGTGGCGTCAAGTCAACATATATGTTTGATCT
GCTGTTGGAGACGAGAAAGCCTGATCAGGTTTTCCAATCTTATAAACCTGGAGAAGTGATGGTGAAAGTT
CATGTTGTTGATCTAAAGGCAGAATCTGTAGCTGCTCCTATAACTGTTCGTGCTTACTTAAATCAGACAG
TTACAGAATTCAAACAACTGATTTCAAAGGCCATCCATTTACCTGCTGAAACAATGAGAATAGTGCTGGA
ACGCTGCTACAATGATTTGCGTCTTCTCAGTGTCTCCAGTAAAACCCTGAAAGCTGAAGGATTTTTTAGA
AGTAACAAGGTGTTTGTTGAAAGCTCCGAGACTTTGGATTACCAGATGGCCTTTGCAGACTCTCATTTAT
GGAAACTCCTGGATCGGCATGCAAATACAATCAGATTATTTGTTTTGCTACCTGAACAATCCCCAGTATC
TTATTCCAAAAGGACAGCATACCAGAAAGCTGGAGGCGATTCTGGTAATGTGGATGATGACTGTGAAAGA
GTCAAAGGACCTGTAGGAAGCCTAAAGTCTGTGGAAGCTATTCTAGAAGAAAGCACTGAAAAACTCAAAA
GCTTGTCACTGCAGCAACAGCAGGATGGAGATAATGGGGACAGCAGCAAAAGTACTGAGACAAGTGACTT
TGAAAACATCGAATCACCTCTCAATGAGAGGGACTCTTCAGCATCAGTGGATAATAGAGAACTTGAACAG
CATATTCAGACTTCTGATCCAGAAAATTTTCAGTCTGAAGAACGATCAGACTCAGATGTGAATAATGACA
GGAGTACAAGTTCAGTGGACAGTGATATTCTTAGCTCCAGTCATAGCAGTGATACTTTGTGCAATGCAGA
CAATGCTCAGATCCCTTTGGCTAATGGACTTGACTCTCACAGTATCACAAGTAGTAGAAGAACGAAAGCA
AATGAAGGGAAAAAAGAAACATGGGATACAGCAGAAGAAGACTCTGGAACTGATAGTGAATATGATGAGA
GTGGCAAGAGTAGGGGAGAAATGCAGTACATGTATTTCAAAGCTGAACCTTATGCTGCAGATGAAGGTTC
TGGGGAAGGACATAAATGGTTGATGGTGCATGTTGATAAAAGAATTACTCTGGCAGCTTTCAAACAACAT
TTAGAGCCCTTTGTTGGAGTTTTGTCCTCTCACTTCAAGGTCTTTCGAGTGTATGCCAGCAATCAAGAGT
TTGAGAGCGTCCGGCTGAATGAGACACTTTCATCATTTTCTGATGACAATAAGATTACAATTAGACTGGG
GAGAGCACTTAAAAAAGGAGAATACAGAGTTAAAGTATACCAGCTTTTGGTCAATGAACAAGAGCCATGC
AAGTTTCTGCTAGATGCTGTGTTTGCTAAAGGAATGACTGTACGGCAATCAAAAGAGGAATTAATTCCTC
AGCTCAGGGAGCAATGTGGTTTAGAGCTCAGTATTGACAGGTTTCGTCTAAGGAAAAAAACATGGAAGAA
TCCTGGCACTGTCTTTTTGGATTATCATATTTATGAAGAAGATATTAATATTTCCAGCAACTGGGAGGTT
TTCCTTGAAGTTCTTGATGGGGTAGAGAAGATGAAGTCCATGTCACAGCTTGCAGTTTTGTCAAGACGGT
GGAAGCCTTCAGAGATGAAGTTGGATCCCTTCCAGGAGGTTGTATTGGAAAGCAGTAGTGTGGACGAATT
GCGAGAGAAGCTTAGTGAAATCAGTGGGATTCCTTTGGATGATATTGAATTTGCTAAGGGTAGAGGAACA
TTTCCCTGTGATATTTCTGTCCTTGATATTCATCAGGATTTAGACTGGAATCCTAAAGTTTCTACCCTGA
ATGTCTGGCCTCTTTATATCTGTGATGATGGTGCGGTCATATTTTATAGGGATAAAACAGAAGAATTAAT
GGAATTGACAGATGAGCAAAGAAATGAACTGATGAAAAAAGAAAGCAGTCGACTCCAGAAGACTGGACAT
CGTGTAACATACTCACCTCGTAAAGAGAAAGCACTAAAAATATATCTGGATGGAGCACCAAATAAAGATC
TGACTCAAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214048 representing NM_017944
Red=Cloning site Green=Tags(s)

MVPGEENQLVPKEAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEQPQILLEDSSAGEDSVHDRFIGPLP
REGSGGSTSDYVSQSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDP
VTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLIN
ELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPNQYFCERCK
KKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSTFIDVEDEKSPQTESCTDSG
AENEGSCHSDQMSNDFSNDDGVDEGICLETNSGTEKISKSGLEKNSLIYELFSVMVHSGSAAGGHYYACI
KSFSDEQWYSFNDQHVSRITQEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPARNAKFLEVDEY
PEHIKNLVQKERELEEQEKRQREIERNTCKIKLFCLHPTKQVMMENKLEVHKDKTLKEAVEMAYKMMDLE
EVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQVFQSYKPGEVMVKV
HVVDLKAESVAAPITVRAYLNQTVTEFKQLISKAIHLPAETMRIVLERCYNDLRLLSVSSKTLKAEGFFR
SNKVFVESSETLDYQMAFADSHLWKLLDRHANTIRLFVLLPEQSPVSYSKRTAYQKAGGDSGNVDDDCER
VKGPVGSLKSVEAILEESTEKLKSLSLQQQQDGDNGDSSKSTETSDFENIESPLNERDSSASVDNRELEQ
HIQTSDPENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADNAQIPLANGLDSHSITSSRRTKA
NEGKKETWDTAEEDSGTDSEYDESGKSRGEMQYMYFKAEPYAADEGSGEGHKWLMVHVDKRITLAAFKQH
LEPFVGVLSSHFKVFRVYASNQEFESVRLNETLSSFSDDNKITIRLGRALKKGEYRVKVYQLLVNEQEPC
KFLLDAVFAKGMTVRQSKEELIPQLREQCGLELSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEV
FLEVLDGVEKMKSMSQLAVLSRRWKPSEMKLDPFQEVVLESSSVDELREKLSEISGIPLDDIEFAKGRGT
FPCDISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELMELTDEQRNELMKKESSRLQKTGH
RVTYSPRKEKALKIYLDGAPNKDLTQD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017944
ORF Size 3861 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017944.4
RefSeq Size 7777 bp
RefSeq ORF 3864 bp
Locus ID 55031
UniProt ID Q96K76
Cytogenetics 11p15.3
Domains UCH
MW 147 kDa
Summary Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.UniProtKB/Swiss-Prot Function
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"NM_017944" in other vectors (6)
SKU Description Size Price
RC214048L1 Lenti-ORF clone of USP47 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 47 (USP47) 10 ug
$1,420.00
RC214048L2 Lenti-ORF clone of USP47 (mGFP-tagged)-Human ubiquitin specific peptidase 47 (USP47) 10 ug
$1,420.00
RC214048L3 Lenti-ORF clone of USP47 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 47 (USP47) 10 ug
$1,420.00
RC214048L4 Lenti-ORF clone of USP47 (mGFP-tagged)-Human ubiquitin specific peptidase 47 (USP47) 10 ug
$1,420.00
RG214048 USP47 (tGFP-tagged) - Human ubiquitin specific peptidase 47 (USP47) 10 ug
$789.00 $1,320.00
SC315333 USP47 (untagged)-Human ubiquitin specific peptidase 47 (USP47) 10 ug
$1,793.00

Plasmid Map for RC214048

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214048
Circular map for RC214048

file-alt Citations

Deubiquitinase USP47-stabilized splicing factor IK regulates the splicing of ATM pre-mRNA
Ka, Lee, Han et al
Cell Death Discov (2020) 6, 34 DOI: 10.1038/s41420-020-0268-1
Cited by 43
AI Summary: OriGene products contributed to this study by providing full-length human IK and USP47 cDNAs, as well as full-length mouse IK cDNA, which were used for plasmid construction and functional assays to investigate the roles of IK and USP47 in ATM pre-mRNA splicing and protein stabilization.
Snippet: … Full-length human IK and USP47 cDNAs were purchased from OriGene (OriGene Technologies, Inc., Rockville, MD) and cloned into pcDNA 3.1, pcDNA 3.0, and pCMV-tag-2B vectors. In the restoration assay, IK cloned into pCMV-tag-2B vector was used;Full-length human IK and USP47 cDNAs were purchased from OriGene (OriGene Technologies, Inc., Rockville, MD) and cloned into pcDNA 3.1, pcDNA 3.0, and pCMV-tag-2B vectors. …
FigureFigureFigure
Showing 1-1 of 1 papers.
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